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BRD3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9602068

Function: Chromatin reader that recognizes and binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling and interaction with transcription factors (PubMed:18406326, PubMed:27105114). Regulates… More details

$325 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
BRD3, Polyclonal Antibody
Full Product Name
BRD3 Antibody
Product Gene Name
Product Synonym Names
BRD 3; Brd3; BRD3_HUMAN; Bromodomain containing 3; Bromodomain containing protein 3; Bromodomain-containing protein 3; KIAA0043; Open reading frame X; ORFX; RING3 like gene; RING3-like protein; RING3L
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Concentration
1mg/ml (lot specific)
Specificity
BRD3 antibody detects endogenous levels of total BRD3
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
BRD3 peptide (MBS9619523) is used for blocking the activity of BRD3 antibody (MBS9602068)
Molecular Weight
Observed: 90 kDa
Predicted: 80 kDa
3D Structure
ModBase 3D Structure for Q15059
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human BRD3
Subcellular Location
Nucleus.
Tissue Specificity
Ubiquitous.
Predicted Cross Reactivity
Pig, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (91%), Bovine (91%), Horse (82%), Rabbit (91%), Dog (91%), Chicken (100%), Xenopus (91%)
Conjugation
Unconjugated
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-BRD3 antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-BRD3 antibody
WB: 1:1000
IHC: 1:200
IF/ICC: 1:100-1:500

Immunohistochemistry (IHC) of anti-BRD3 antibody

MBS9602068 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Rabbit Anti-BRD3 Polyclonal Antibody – Cat# MBS9602068

Immunofluorescene (IF) of anti-BRD3 antibody

MBS9602068 staining A549 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
Rabbit Anti-BRD3 Polyclonal Antibody, Immunofluorescene (IF) – Cat# MBS9602068

Western Blot (WB) of anti-BRD3 antibody

Western blot analysis of BRD3 using A549 whole cell lysates
Rabbit Anti-BRD3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS9602068

Western Blot (WB) of anti-BRD3 antibody

Western blot analysis of extracts from 293, using BRD3 Antibody. Lane 1 was treated with the blocking peptide.
Rabbit Anti-BRD3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS9602068

Other Notes

Small volumes of anti-BRD3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BRD3 purchase

MBS9602068 is an antibody targeting BRD3. Also known as BRD3 Antibody. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for BRD3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
bromodomain-containing protein 3
NCBI Official Synonym Full Names
bromodomain containing 3
NCBI Official Symbol
BRD3
NCBI Official Synonym Symbols
ORFX; RING3L
UniProt Protein Name
Bromodomain-containing protein 3
UniProt Synonym Protein Names
RING3-like protein
UniProt Gene Name
BRD3
UniProt Synonym Gene Names
KIAA0043; RING3L
NCBI Protein Information
bromodomain-containing protein 3

NCBI Summary for BRD3

This gene was identified based on its homology to the gene encoding the RING3 protein, a serine/threonine kinase. The gene localizes to 9q34, a region which contains several major histocompatibility complex (MHC) genes. The function of the encoded protein is not known. [provided by RefSeq, Jul 2008]

UniProt Comments for BRD3

Chromatin reader that recognizes and binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling and interaction with transcription factors (PubMed:18406326, PubMed:27105114). Regulates transcription by promoting the binding of the transcription factor GATA1 to its targets ().
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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