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C5 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS176937

Description: Rabbit IgG polyclonal antibody for Complement C5(C5) detection. Tested with WB, IHC-P in Mouse, Rat.Background: C5a is a protein fragment released from complement component C5. This gene is mapped to 9q33.2. The protein encoded by this gene is… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
C5/C5a, Polyclonal Antibody
Full Product Name
Anti-C5/C5a antibody
Product Gene Name
anti-C5 antibody
Product Synonym Names
Complement C5; complement component 5; Anaphylatoxin C5a analog antibody; Anaphylatoxin C5a antibody; C5 antibody; C5a antibody; Complement C5 antibody; Complement component C5 antibody; Complement component C5a antibody; CPAMD 4 antibody; CPAMD4 antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse, Rat
Form/Format
Lyophilized
Purity/Purification
Immunogen affinity purified.
Specificity
No cross reactivity with other proteins.
Preparation and Storage
Store at -20°C. After reconstitution, store at 4°C. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
3D Structure
ModBase 3D Structure for P08650
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of rat C5a (1-18aa DLQLLHQKVEEQAAKYKH), different from the related mouse sequence by four amino acids.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-C5 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-C5 antibody
Western Blot (WB)
Concentration: 0.1-0.5ug/ml
Tested Species: Ms, Rat

Immunohistochemistry-Paraffin-embedded Section (IHC-P)
Concentration: 0.5-1ug/ml
Tested Species: MS, Rat
Antigen Retrieval: By Heat

Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.

By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20 minutes is required for the staining of fomalin/paraffin sections.

Other applications have not been tested.
Optimal dilutions should be determined by end users.

Immunohistochemistry (IHC) of anti-C5 antibody

Anti-C5/C5a antibody, MBS176937, IHC(P)
IHC(P): Mouse Spleen Tissue
Rabbit Anti-C5 Polyclonal Antibody – Cat# MBS176937

Immunohistochemistry (IHC) of anti-C5 antibody

Anti-C5/C5a antibody, MBS176937, IHC(P)
IHC(P): Rat Liver Tissue
Rabbit Anti-C5 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS176937

Immunohistochemistry (IHC) of anti-C5 antibody

Anti-C5/C5a antibody, MBS176937, IHC(P)
IHC(P): Rat Spleen Tissue
Rabbit Anti-C5 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS176937

Western Blot (WB) of anti-C5 antibody

Anti-C5/C5a antibody, MBS176937, All Western blotting
All lanes: Anti-C5a(MBS176937) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 40ug
Lane 2: Mouse Liver Tissue Lysate at 40ug
Lane 3: NIH Whole Cell Lysate at 40ug
Lane 4: HEPA Whole Cell Lysate at 40ug
Lane 5: PC12 Whole Cell Lysate at 40ug
Predicted bind size: 115KD
Observed bind size: 115KD
Rabbit Anti-C5 Polyclonal Antibody, Western Blot (WB) – Cat# MBS176937

Other Notes

Small volumes of anti-C5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for C5 purchase

MBS176937 is an antibody targeting C5. Also known as Anti-C5/C5a antibody. Reactive in Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for C5. It may not necessarily be applicable to this product.

NCBI GI #
46397770
NCBI GeneID
362119
NCBI Accession #
P08650.2
UniProt Primary Accession #
P08650
UniProt Secondary Accession #
Q63078
UniProt Related Accession #
P08650
NCBI Official Full Name
Complement C5
NCBI Official Synonym Full Names
complement component 5
NCBI Official Symbol
C5
NCBI Official Synonym Symbols
Hc; C5a; RGD1561905
UniProt Protein Name
Complement C5
Protein Family
Complement C5
UniProt Gene Name
C5
UniProt Entry Name
CO5_RAT
NCBI Protein Information
complement C5; anaphylatoxin

NCBI Summary for C5

likely component of the classical pathway of the complement cascade; may be involved with tissue injury associated with ischemia or sepsis; may be a component of the membrane attack complex [RGD, Feb 2006]

UniProt Comments for C5

C5: Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. Defects in C5 are the cause of complement component 5 deficiency (C5D). A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele (PubMed:15995705).

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space

Molecular Function: C5a anaphylatoxin chemotactic receptor binding

Biological Process: cellular calcium ion homeostasis; positive regulation of angiogenesis; positive regulation of chemotaxis; in utero embryonic development; complement activation, alternative pathway; glucose homeostasis; chemotaxis; inflammatory response; negative regulation of dopamine secretion; complement activation, classical pathway; leukocyte migration during inflammatory response
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Research Articles on C5

1. data indicate that the mechanism of IL-6 and TGF-beta1 production in renal tissue of Thy-1N rats is associated with sublytic C5b-9 up-regulated p300 and p300-mediated C/EBPbeta acetylation

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-C5 antibody

 Products by Pathway  Pathway Diagram
 Activation Of C3 And C5 Pathway antibodies  Activation Of C3 And C5 Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Complement Activation, Classical Pathway antibodies  Complement Activation, Classical Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement Cascade Pathway antibodies  Complement Cascade Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Herpes Simplex Infection Pathway antibodies  Herpes Simplex Infection Pathway Diagram

Organs/Tissues associated with anti-C5 antibody

 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >461 publications with C5 and Kidney
 Liver Antibodies  >432 publications with C5 and Liver
 Embryonic Tissue Antibodies  >63 publications with C5 and Embryonic Tissue
 Adrenal Gland Antibodies  >10 publications with C5 and Adrenal Gland
 Uncharacterized Tissue Antibodies  >2 publications with C5 and Uncharacterized Tissue
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