Rabbit Anti-USP25 Polyclonal Antibody – Cat# MBS840586

USP25 Antibody – Rabbit Polyclonal

Name: USP25 Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS840586
Price: 475 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS840586

Polyclonal antibody to detect USP25 (a DUB enzyme) in human, mouse and rat samples.Background: Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzymes (DUB) action. There are five DUB… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

USP25, Polyclonal Antibody

Full Product Name

USP25 Antibody

Product Gene Name

anti-USP25 antibody

Product Synonym Names

USP25; USP21

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Form/Format

100 ug of antibody in 100 ul PBS containing 0.02% sodium azide, 50% glycerol, pH 7.3
Appearance: Colorless liquid

Purity/Purification

Affinity purified

Preparation and Storage

At -20 degree C
Shelf Life: 12 months

Molecular Weight

129,963 Da

OMIM

604736

3D Structure

ModBase 3D Structure for Q9UHP3

Immunogen

Fusion protein of human USP25

Positive Control

Western Blot: BxPC-3 cell lysate

Handling

The antibody solution should be gently mixed before use.

Product Categories/Family for anti-USP25 antibody

Epigenetics; Eraser Enzymes; Ubiquitin-Specific Proteases; AntibodiesAntibodies & Supporting Tools; Primary Antibodies (A-Z)

Applications Tested/Suitable for anti-USP25 antibody

Western Blot (WB)

Application Notes for anti-USP25 antibody

Western blot: 1:500 - 1:1000 dilution. However, the optimal conditions should be determined individually.

Other Notes

Small volumes of anti-USP25 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for USP25 purchase

MBS840586 is an antibody targeting USP25. Also known as USP25 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for USP25. It may not necessarily be applicable to this product.

NCBI GI #

545688691

NCBI GeneID

29761

NCBI Accession #

NP_001269970.1

UniProt Primary Accession #

Q9UHP3

UniProt Secondary Accession #

Q6DHZ9; Q9H9W1; C0LSZ0

UniProt Related Accession #

Q9UHP3

GenBank Nucleotide #

NM_001283041.1

NCBI Official Full Name

ubiquitin carboxyl-terminal hydrolase 25 isoform USP25m

NCBI Official Synonym Full Names

ubiquitin specific peptidase 25

NCBI Official Symbol

USP25

NCBI Official Synonym Symbols

USP21

UniProt Protein Name

Ubiquitin carboxyl-terminal hydrolase 25

UniProt Synonym Protein Names

Deubiquitinating enzyme 25; USP on chromosome 21; Ubiquitin thioesterase 25; Ubiquitin-specific-processing protease 25

UniProt Gene Name

USP25

UniProt Synonym Gene Names

USP21

UniProt Entry Name

UBP25_HUMAN

NCBI Protein Information

ubiquitin carboxyl-terminal hydrolase 25

NCBI Summary for USP25

Ubiquitin (MIM 191339) is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response. Ubiquitin is released from degraded proteins by disassembly of the polyubiquitin chains, which is mediated by ubiquitin-specific proteases (USPs), such as USP25 (Valero et al., 1999 [PubMed 10644437]).[supplied by OMIM, Mar 2008]

UniProt Comments for USP25

USP25: Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains. Belongs to the peptidase C19 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.19.12; Protease; Ubiquitin conjugating system; Ubiquitin-specific protease

Chromosomal Location of Human Ortholog: 21q11.2

Cellular Component: cytoplasm; nucleus

Molecular Function: peptidase activity; protein binding; SUMO binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein modification process; proteolysis

Research Articles on USP25

1. Findings suggest that ubiquitin-specific protease 25 (USP25) as a VRK2 substrate that acts on TRiC deubiquitination.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-USP25 antibody

Reference Product	PubMed Publications
SUMO2 antibody	>3 publications with USP25 and SUMO2
SUMO1 antibody	>1 publications with USP25 and SUMO1
SYK antibody	>1 publications with USP25 and SYK

Diseases associated with anti-USP25 antibody

Disease Name	Pubmed Publications
Lung Neoplasms Antibodies	>2 publications with USP25 and Lung Neoplasms
Congenital Abnormalities Antibodies	>2 publications with USP25 and Congenital Abnormalities
Neurobehavioral Manifestations Antibodies	>2 publications with USP25 and Neurobehavioral Manifestations
Nervous System Diseases Antibodies	>2 publications with USP25 and Nervous System Diseases
Inflammation Antibodies	>2 publications with USP25 and Inflammation
Fatty Liver Antibodies	>1 publications with USP25 and Fatty Liver
Breast Neoplasms Antibodies	>1 publications with USP25 and Breast Neoplasms
Liver Diseases Antibodies	>1 publications with USP25 and Liver Diseases
Hyperplasia Antibodies	>1 publications with USP25 and Hyperplasia
Fibrosis Antibodies	>1 publications with USP25 and Fibrosis

Organs/Tissues associated with anti-USP25 antibody

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>4 publications with USP25 and Muscle
Lung Antibodies	>2 publications with USP25 and Lung
Testis Antibodies	>2 publications with USP25 and Testis
Heart Antibodies	>1 publications with USP25 and Heart
Eye Antibodies	>1 publications with USP25 and Eye
Liver Antibodies	>1 publications with USP25 and Liver
Blood Antibodies	>1 publications with USP25 and Blood
Embryonic Tissue Antibodies	>1 publications with USP25 and Embryonic Tissue
Lymph Node Antibodies	>1 publications with USP25 and Lymph Node
Brain Antibodies	>1 publications with USP25 and Brain