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Perforin (PRF1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS840595

Background: Human perforin (cytolysin) is believed to be one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis. Perforin expression is constitutive on NK cells, but increases in resting XD8+ cytotoxic cells… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Product Overview

Product Name
Perforin (Prf1), Polyclonal Antibody
Full Product Name
Perforin Antibody
Product Gene Name
Product Synonym Names
PFP; P1; FLH2; HPLH2; PRF1; Cytolysin; MGC65093
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
100 ug (0.5 mg/ml) affinity purified rabbit anti-Perforin polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Appearance: Colorless liquid
Purity/Purification
Affinity purified
Concentration
0.5 mg/ml (lot specific)
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Molecular Weight
61,513 Da
Immunogen
Synthetic peptide surrounding amino acid 568 of rat Perforin
Handling
The antibody solution should be gently mixed before use.
Applications Tested/Suitable for anti-Prf1 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes for anti-Prf1 antibody
Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually. The antibody detects ~70 kDa Perforin in samples from human, mouse and rat origins. Reactivity to other species has not been determined.

Other Notes

Small volumes of anti-Prf1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Prf1 purchase

MBS840595 is an antibody targeting Prf1. Also known as Perforin Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Prf1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
perforin-1
NCBI Official Synonym Full Names
perforin 1 (pore forming protein)
NCBI Official Symbol
Prf1
NCBI Official Synonym Symbols
Cyta; RATCYTA
UniProt Protein Name
Perforin-1
UniProt Synonym Protein Names
Cytolysin; Lymphocyte pore-forming protein
UniProt Gene Name
Prf1
UniProt Synonym Gene Names
Pfp; P1
UniProt Entry Name
PERF_RAT
NCBI Protein Information
perforin-1

NCBI Summary for Prf1

cytolytic factor present in cytoplasmic granules [RGD, Feb 2006]

UniProt Comments for Prf1

Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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