Rabbit Anti-ASAH2 Polyclonal Antibody – Cat# MBS840664

ASAH2 Antibody – Rabbit Polyclonal

Name: ASAH2 Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS840664
Price: 215 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS840664

Background: ASAH2 is a key regulator of sphingolipid signaling metabolites. It catalyzes the degradation of sphingolipid C-16 Ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

ASAH2, Polyclonal Antibody

Full Product Name

ASAH2 Antibody

Product Gene Name

anti-ASAH2 antibody

Product Synonym Names

ASAH-2; HNAC1; MGC129777

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Form/Format

100 ug (0.5 mg/ml) affinity purified rabbit anti-ASAH-2 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Appearance: Colorless liquid

Purity/Purification

Affinity purified

Concentration

0.5 mg/ml (lot specific)

Preparation and Storage

At -20 degree C
Shelf Life: 12 months

Molecular Weight

81,718 Da

OMIM

611202

3D Structure

ModBase 3D Structure for Q9NR71

Immunogen

Synthetic peptide surrounding amino acid 741 of human ASAH-2

Handling

The antibody solution should be gently mixed before use.

Applications Tested/Suitable for anti-ASAH2 antibody

Western Blot (WB)

Application Notes for anti-ASAH2 antibody

Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually. Other applications have not been determined.

Other Notes

Small volumes of anti-ASAH2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ASAH2 purchase

MBS840664 is an antibody targeting ASAH2. Also known as ASAH2 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ASAH2. It may not necessarily be applicable to this product.

NCBI GI #

221307475

NCBI GeneID

56624

NCBI Accession #

NP_001137446.1

UniProt Primary Accession #

Q9NR71

UniProt Secondary Accession #

Q3KNU1; Q5SNT7; Q5SZP6; Q5SZP7; Q5T1D5; Q71ME6

UniProt Related Accession #

Q9NR71

GenBank Nucleotide #

NM_001143974.1

NCBI Official Full Name

neutral ceramidase isoform b

NCBI Official Synonym Full Names

N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2

NCBI Official Symbol

ASAH2

NCBI Official Synonym Symbols

HNAC1; BCDase; LCDase; NCDase; N-CDase

UniProt Protein Name

Neutral ceramidase

UniProt Synonym Protein Names

Acylsphingosine deacylase 2; BCDase; LCDase; hCD

Protein Family

Neutral ceramidase

UniProt Gene Name

ASAH2

UniProt Synonym Gene Names

HNAC1; N-CDase; NCDase; hCD

UniProt Entry Name

ASAH2_HUMAN

NCBI Protein Information

neutral ceramidase

NCBI Summary for ASAH2

Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger (see MIM 603730) (Mitsutake et al., 2001 [PubMed 11328816]).[supplied by OMIM, Mar 2008]

UniProt Comments for ASAH2

ASAH2: Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract. Belongs to the neutral ceramidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - sphingolipid; Membrane protein, integral; EC 3.5.1.23; Hydrolase

Chromosomal Location of Human Ortholog: 10q11.21

Cellular Component: mitochondrion; plasma membrane; integral to membrane

Molecular Function: ceramidase activity

Biological Process: sphingolipid metabolic process; apoptosis; glycosphingolipid metabolic process; ceramide metabolic process; signal transduction

Research Articles on ASAH2

1. These results demonstrate for the first time that adiponectin inhibits TNFalpha-induced inflammatory response via Cav1-mediated ceramidase recruitment and activation in an AdipoR1-dependent fashion.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ASAH2 antibody

Reference Product	PubMed Publications
ASAH1 antibody	>92 publications with ASAH2 and ASAH1
SMPD1 antibody	>53 publications with ASAH2 and SMPD1
SPHK2 antibody	>3 publications with ASAH2 and SPHK2
SPHK1 antibody	>3 publications with ASAH2 and SPHK1
SMPD3 antibody	>1 publications with ASAH2 and SMPD3

Pathways associated with anti-ASAH2 antibody

Products by Pathway	Pathway Diagram
Glycosphingolipid Metabolism Pathway antibodies	Glycosphingolipid Metabolism Pathway Diagram
Metabolic Pathways antibodies	Metabolic Pathways Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Lipids And Lipoproteins Pathway antibodies	Metabolism Of Lipids And Lipoproteins Pathway Diagram
Sphingolipid Metabolism Pathway antibodies	Sphingolipid Metabolism Pathway Diagram
Sphingolipid Metabolism Pathway antibodies	Sphingolipid Metabolism Pathway Diagram
Sphingolipid Metabolism Pathway antibodies	Sphingolipid Metabolism Pathway Diagram
Sphingolipid Metabolism Pathway antibodies	Sphingolipid Metabolism Pathway Diagram
Sphingolipid Signaling Pathway antibodies	Sphingolipid Signaling Pathway Diagram
Sphingolipid Signaling Pathway antibodies	Sphingolipid Signaling Pathway Diagram

Diseases associated with anti-ASAH2 antibody

Disease Name	Pubmed Publications
Necrosis Antibodies	>13 publications with ASAH2 and Necrosis
Inflammation Antibodies	>8 publications with ASAH2 and Inflammation
Nervous System Diseases Antibodies	>6 publications with ASAH2 and Nervous System Diseases
Fatty Liver Antibodies	>4 publications with ASAH2 and Fatty Liver
Disease Models, Animal Antibodies	>4 publications with ASAH2 and Disease Models, Animal
Cardiovascular Diseases Antibodies	>3 publications with ASAH2 and Cardiovascular Diseases
Kidney Diseases Antibodies	>3 publications with ASAH2 and Kidney Diseases
Drug-Induced Liver Injury Antibodies	>2 publications with ASAH2 and Drug-Induced Liver Injury
Hyperplasia Antibodies	>1 publications with ASAH2 and Hyperplasia
Drug-Related Side Effects and Adverse Reactions Antibodies	>1 publications with ASAH2 and Drug-Related Side Effects and Adverse Reactions

Organs/Tissues associated with anti-ASAH2 antibody

Organ/Tissue Name	Pubmed Publications
Skin Antibodies	>3 publications with ASAH2 and Skin
Mixed Antibodies	>1 publications with ASAH2 and Mixed