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His-Tag Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS840765

Background: A variety of plasmids contain DNA that encodes an N-terminal tag consisting of six histidine (His) residues, followed by an extended multiple cloning sites. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
His-Tag, Polyclonal Antibody
Full Product Name
His-Tag Antibody
Product Synonym Names
His tag; HHHHHH tag
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
All species
Form/Format
100 ug (0.5 mg/ml) peptide affinity purified rabbit anti-His-Tag polyclonal antibody in phosphate buffered saline (PBS, pH 7.2), containing 50% glycerol, 1% BSA, 0.02% thimerosal.
Appearance: Colorless liquid
Purity/Purification
Affinity purified
Concentration
0.5 mg/ml (lot specific)
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Immunogen
6xHis synthetic peptide
Handling
The antibody solution should be gently mixed before use.
Applications Tested/Suitable for anti-His-Tag antibody
Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
Application Notes for anti-His-Tag antibody
Western blotting (1 ug/ml), immunoprecipitation (40 ug/ml), and immunocytochemistry (2 ug/ml). However, the optimal conditions should be determined individually. The antibody detects His-tagged recombinant proteins.

Testing Data of anti-His-Tag antibody

Rabbit Anti-His-Tag Polyclonal Antibody – Cat# MBS840765

Other Notes

Small volumes of anti-His-Tag antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for His-Tag purchase

MBS840765 is an antibody targeting His-Tag. Also known as His-Tag Antibody. Reactive in All species. Host: Rabbit.
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