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Histone H3ac (H3AC) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS388989

Short Description: Our Histone H3ac (pan-acetyl) antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, Dot blot, Immunofluorescence and Western blot; it has been shown to react with Human samples, but it… More details

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Product Overview

Online Datasheet in PDF
Product Name
Histone H3ac, Polyclonal Antibody
Full Product Name
Histone H3ac (pan-acetyl) antibody (pAb)
Product Gene Name
anti-H3ac antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Wide Range Predicted
Form/Format
PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, a sera version of this antibody is also available.
Purity/Purification
Protein A Chromatography
Concentration
1ug/ul (lot specific)
Preparation and Storage
Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for 1 year. Keep all reagents on ice when not in storage.
Molecular Weight
17kDa
Immunogen
This antibody was raised against a peptide including acetyl-lysines contained in the N-terminal tail of human Histone H3.

Product Categories/Family for anti-H3ac antibody

Primary Antibodies
Applications Tested/Suitable for anti-H3ac antibody
Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-H3ac antibody
Applications Validated:
ChIP: 10ug per ChIP
WB: 0.2-1ug/ml dilution
IF: 1:500 dilution
Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western Blot.

Chromatin Immunoprecipitation (ChIP) of anti-H3ac antibody

H3ac (pan-acetyl) antibody (pAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 13 ug of chromatin from both HeLa cells and 10 ug H3ac (pan-acetyl) antibody. ChIP DNA was used in qPCR with the gene-specific primer as indicated. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.
Rabbit Anti-H3AC Polyclonal Antibody – Cat# MBS388989

Western Blot (WB) of anti-H3ac antibody

Histone H3ac (pan-acetyl) antibody (pAb) tested by Western blot. Detection of acetylated Histone H3 by Western blot analysis using 20 ug HeLa nuclear extract and Histone H3ac (pan-acetyl) antibody at a 1 ug/ml dilution. Lane 1: Nuclear extract of untreated HeLa cells. Lane 2: Nuclear extract of HeLa cells treated with sodium butyrate.
Rabbit Anti-H3AC Polyclonal Antibody, Western Blot (WB) – Cat# MBS388989

Dot Blot (DB) of anti-H3ac antibody

Histone H3ac (pan-acetyl)antibody (pAb) tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3ac antibody. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 1 ug/ml. The amount of peptide (picomoles) spotted is indicated next to the row. Lane 1: H3K4ac peptide. Lane 2: unmodified H3K4 peptide. Lane 3: H3K9ac peptide. Lane 4: unmodified H3K9 peptide. Lane 5: H3K18ac peptide. Lane 6: unmodified H3K18 peptide. Lane 7: H3K23ac peptide. Lane 8: unmodified H3K23 peptide. Lane 9: H3K27ac peptide. Lane 10: unmodified H3K27 peptide.
Rabbit Anti-H3AC Polyclonal Antibody, Dot Blot (DB) – Cat# MBS388989

Immunofluorescence (IF) of anti-H3ac antibody

Detection of H3panAc by immunofluorescence. U2OS cells were stained with H3panAc antibody at a dilution of 1:500. Left panel: DAPI. Middle panel: Histone H3 pan-acetyl antibody staining. Right panel: merge.
Rabbit Anti-H3AC Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS388989

Other Notes

Small volumes of anti-H3ac antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for H3ac purchase

MBS388989 is an antibody targeting H3ac. Also known as Histone H3ac (pan-acetyl) antibody (pAb). Reactive in Human, Wide Range Predicted. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for H3ac. It may not necessarily be applicable to this product.

NCBI Accession #
NP_003522
NCBI Related Accession #
AB_2793714

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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