DNMT1 Antibody – Rabbit Polyclonal

DNMT1: an ubiquitous DNA methyltransferase that methylates CpG residues. Preferentially methylates hemimethylated DNA. It is responsible for maintaining methylation patterns established in development, and may play an active role in DNA damage repair. Mediates transcriptional repression by direct binding to HDAC2. Its abundance is reduced to non detectable levels at the G0 phase of the cell cycle and is dramatically induced upon entrance into the S-phase of the cell cycle. Interacts with HDAC1 and with PCNA. Forms a complex with DMAP1 and HDAC2, with direct interaction. Forms also a stable complex with E2F1, BB1 and HDAC1. Binds MBD2 and MBD3. Three isoforms of the human protein produced by alternative splicing have been described.

Protein type: Amino Acid Metabolism - cysteine and methionine; Transcription regulation; Methyltransferase; Cell development/differentiation; Methyltransferase, DNA; EC 2.1.1.37

Cellular Component: heterochromatin; centric heterochromatin; protein complex; cell soma; cytoplasm; replication fork; nucleus

Molecular Function: methyltransferase activity; protein domain specific binding; methyl-CpG binding; unmethylated CpG binding; zinc ion binding; RNA binding; histone deacetylase binding; S-adenosylmethionine-dependent methyltransferase activity; DNA-methyltransferase activity; transcription factor binding; DNA (cytosine-5-)-methyltransferase activity; protein binding; DNA binding; DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates; double-stranded DNA binding; estrogen receptor binding; chromatin binding

Biological Process: maintenance of DNA methylation; response to toxin; S-adenosylhomocysteine metabolic process; response to lipopolysaccharide; negative regulation of transcription from RNA polymerase II promoter; positive regulation of histone H3-K4 methylation; gene silencing; response to estradiol stimulus; response to caffeine; response to vitamin A; neuron differentiation; response to organic substance; regulation of transcription, DNA-dependent; DNA methylation; DNA methylation on cytosine; aging; response to nutrient levels; response to drug; negative regulation of histone H3-K9 methylation; transcription, DNA-dependent; S-adenosylmethionine metabolic process; response to testosterone stimulus; chromatin modification; regulation of cell proliferation; cytosine methylation within a CG sequence; response to ethanol; response to heat; regulation of gene expression; response to lead ion; S-adenosylmethioninamine metabolic process; response to activity; response to ionizing radiation; brain development; negative regulation of transcription, DNA-dependent