Rabbit Anti-SETD1B Polyclonal Antibody – Cat# MBS840858

SETD1B Antibody – Rabbit Polyclonal

Name: SETD1B Antibody; Polyclonal; Rabbit; Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS840858
Price: 315 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS840858

Polyclonal Antibody to detect Setd1b in mouse samples. Background: SETD1B (UniProt/Swiss-Prot entry Q9UPS6) is a component of the SET1 histone methyltransferase (HMT) complex. This complex specifically methylates lysine 4 of histone H3, but only if the… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

Setd1b, Polyclonal Antibody

Full Product Name

Setd1b polyclonal antibody

Product Gene Name

anti-Setd1b antibody

Product Synonym Names

SET1B; KMT2G

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Mouse

Form/Format

In PBS with 0.05% (W/V) sodium azide.
Appearance: Colorless liquid

Preparation and Storage

At -20 degree C
Shelf Life: 12 months

Molecular Weight

208,732 Da

OMIM

611055

3D Structure

ModBase 3D Structure for Q9UPS6

Immunogen

Polyclonal antibody raised in rabbit against mouse Setd1b (Set domain containing 1B), using a 3 KLH-conjugated synthetic peptides containing sequences from the central part of the protein

Positive Control

Western blot: NIH3T3 and embryonic stem cells, ELISA: Peptides.

Handling

The antibody solution should be gently mixed before use.

Product Categories/Family for anti-Setd1b antibody

Epigenetics; Writer Enzymes; DNA Methyltransferases (DNMTs); Antibodies and Blocking PeptidesAntibodies & Supporting Tools; Antibodies for Epigenetics ResearchAntibodies & Supporting Tools; Primary Antibodies (A-Z)

Applications Tested/Suitable for anti-Setd1b antibody

Western Blot (WB), ELISA (EIA)

Application Notes for anti-Setd1b antibody

ELISA: 1:100 - 1:500, Western Blot: 1:500.

Other Notes

Small volumes of anti-Setd1b antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Setd1b purchase

MBS840858 is an antibody targeting Setd1b. Also known as Setd1b polyclonal antibody. Reactive in Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Setd1b. It may not necessarily be applicable to this product.

NCBI GI #

210032580

NCBI GeneID

23067

NCBI Accession #

NP_055863.1

UniProt Primary Accession #

Q9UPS6

UniProt Secondary Accession #

F6MFW1

UniProt Related Accession #

Q9UPS6

GenBank Nucleotide #

NM_015048.1

NCBI Official Full Name

histone-lysine N-methyltransferase SETD1B

NCBI Official Synonym Full Names

SET domain containing 1B

NCBI Official Symbol

SETD1B

NCBI Official Synonym Symbols

KMT2G; Set1B

UniProt Protein Name

Histone-lysine N-methyltransferase SETD1B

UniProt Synonym Protein Names

Lysine N-methyltransferase 2G; SET domain-containing protein 1B; hSET1B

Protein Family

Histone-lysine N-methyltransferase

UniProt Gene Name

SETD1B

UniProt Synonym Gene Names

KIAA1076; KMT2G; SET1B; hSET1B

UniProt Entry Name

SET1B_HUMAN

NCBI Protein Information

histone-lysine N-methyltransferase SETD1B

NCBI Summary for Setd1b

SET1B is a component of a histone methyltransferase complex that produces trimethylated histone H3 at Lys4 (Lee et al., 2007 [PubMed 17355966]).[supplied by OMIM, Mar 2008]

UniProt Comments for Setd1b

SETD1B: Histone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys- 9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overalpping localization with SETD1A suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression. Specifically tri-methylates 'Lys-4' of histone H3 in vitro.

Protein type: Methyltransferase, protein lysine; Amino Acid Metabolism - lysine degradation; Methyltransferase; EC 2.1.1.43

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: nucleoplasm; histone methyltransferase complex; cytoplasm; nuclear speck; chromosome

Molecular Function: protein binding; histone lysine N-methyltransferase activity (H3-K4 specific); RNA binding; nucleotide binding

Biological Process: establishment and/or maintenance of chromatin architecture; regulation of transcription, DNA-dependent; transcription, DNA-dependent; histone H3-K4 methylation

Research Articles on Setd1b

1. We analyzed SETD1B expression in gastric and colorectal cancer. Loss of SETD1B was identified in 15% to 55% of the GC and CRC with respect to the microsatellite instability status. Loss of expression was more common in those with SETD1B mutations.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.