Rabbit Anti-TRADD Polyclonal Antibody – Cat# MBS840894

TRADD Antibody – Rabbit Polyclonal

Name: TRADD Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS840894
Price: 215 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS840894

Background: TRADD (TNF-R1-associated death domain) is a 34 kDa protein that associates with the death domains of both FAS and TNF-R1 via their DDH regions. Overexpression of TRADD leads to NFkappaB activation and apoptosis in the absence of TNF. More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

TRADD, Polyclonal Antibody

Full Product Name

TRADD Antibody

Product Gene Name

anti-TRADD antibody

Product Synonym Names

MGC11078; Hs.89862; TNFR1

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Form/Format

100 ug (0.2 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
Appearance: Colorless liquid

Purity/Purification

Affinity purified

Concentration

0.2 mg/ml (lot specific)

Preparation and Storage

At -20 degree C
Shelf Life: 12 months

Molecular Weight

27,904 Da

OMIM

603500

3D Structure

ModBase 3D Structure for Q15628

Immunogen

Synthetic peptide surrounding amino acid 300 of human TRADD

Handling

The antibody solution should be gently mixed before use.

Applications Tested/Suitable for anti-TRADD antibody

Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC)

Application Notes for anti-TRADD antibody

Western blotting (0.5-4 ug/ml). Based on researchers feedback, it also works well in immunoprecipitation (10-20 ug/ml) and Immunohistochemistry (15-25 ug/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 34 kDa TRADD in samples from human, mouse, and rat origins. Reactivity to other species has not been tested. Mouse small intestine tissue lysate can be used as a positive control.

Other Notes

Small volumes of anti-TRADD antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TRADD purchase

MBS840894 is an antibody targeting TRADD. Also known as TRADD Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TRADD. It may not necessarily be applicable to this product.

NCBI GI #

13378137

NCBI GeneID

8717

NCBI Accession #

NP_003780.1

UniProt Primary Accession #

Q15628

UniProt Secondary Accession #

Q52NZ1; B2RDS3; B3KQZ9

UniProt Related Accession #

Q15628

GenBank Nucleotide #

NM_003789.3

NCBI Official Full Name

tumor necrosis factor receptor type 1-associated DEATH domain protein

NCBI Official Synonym Full Names

TNFRSF1A-associated via death domain

NCBI Official Symbol

TRADD

NCBI Official Synonym Symbols

Hs.89862

UniProt Protein Name

Tumor necrosis factor receptor type 1-associated DEATH domain protein

UniProt Synonym Protein Names

TNFRSF1A-associated via death domain

Protein Family

Tumor necrosis factor receptor type 1-associated DEATH domain protein

UniProt Gene Name

TRADD

UniProt Synonym Gene Names

TNFR1-associated DEATH domain protein

UniProt Entry Name

TRADD_HUMAN

NCBI Protein Information

tumor necrosis factor receptor type 1-associated DEATH domain protein

NCBI Summary for TRADD

The protein encoded by this gene is a death domain containing adaptor molecule that interacts with TNFRSF1A/TNFR1 and mediates programmed cell death signaling and NF-kappaB activation. This protein binds adaptor protein TRAF2, reduces the recruitment of inhibitor-of-apoptosis proteins (IAPs) by TRAF2, and thus suppresses TRAF2 mediated apoptosis. This protein can also interact with receptor TNFRSF6/FAS and adaptor protein FADD/MORT1, and is involved in the Fas-induced cell death pathway. [provided by RefSeq, Jul 2008]

UniProt Comments for TRADD

TRADD: Adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B. Heterodimer with TNFRSF1A/TNFR1. Interacts with DAB2IP, FADD, HIPK2, KRT14, KRT16, KRT17, KRT18, RIPK1, SQSTM1, TRAF1, TRAF2 and TRPC4AP. Found in all examined tissues.

Protein type: Adaptor/scaffold; Apoptosis

Chromosomal Location of Human Ortholog: 16q22

Cellular Component: cytoskeleton; cytoplasm; cytosol; nucleus; receptor complex; lipid raft

Molecular Function: identical protein binding; protein binding; signal transducer activity; protein complex binding; tumor necrosis factor receptor binding; kinase binding; molecular adaptor activity

Biological Process: caspase activation; positive regulation of hair follicle development; positive regulation of I-kappaB kinase/NF-kappaB cascade; tumor necrosis factor-mediated signaling pathway; induction of apoptosis via death domain receptors; protein heterooligomerization; apoptosis; positive regulation of apoptosis; signal transduction; activation of NF-kappaB transcription factor

Research Articles on TRADD

1. Biologic assessment found that NPM-RAR expression impaired TNF-induced signaling through TRADD, blunting TNF-mediated activation of caspase-3 (CASP3) and caspase-8 (CASP8), to ultimately block apoptosis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.