Rabbit Anti-EndoG Polyclonal Antibody – Cat# MBS840898

EndoG Antibody – Rabbit Polyclonal

Name: EndoG Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS840898
Price: 215 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS840898

Background: Endonuclease G (EndoG) is a mitochondrion-specific nuclease that specifically activated by apoptotic stimuli and translocates to the nucleus during apoptosis. In nucleus, EndoG induces nucleosomal fragmentation of DNA independent of caspase and… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

EndoG, Polyclonal Antibody

Full Product Name

EndoG Antibody

Product Gene Name

anti-EndoG antibody

Product Synonym Names

ENDOG; FLJ27463

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Form/Format

100 ug (0.5 mg/ml) affinity purified rabbit anti-EndoG polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Appearance: Colorless liquid

Purity/Purification

Affinity purified

Concentration

0.5 mg/ml (lot specific)

Preparation and Storage

At -20 degree C
Shelf Life: 12 months

Molecular Weight

32,620 Da

OMIM

600440

Immunogen

Synthetic peptide surrounding amino acid 280 of rat EndoG

Handling

The antibody solution should be gently mixed before use.

Product Categories/Family for anti-EndoG antibody

Molecular Biology Tools; Molecular Biology Antibodies & Blocking Peptides

Applications Tested/Suitable for anti-EndoG antibody

Western Blot (WB)

Application Notes for anti-EndoG antibody

Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually. The antibody recognizes ~35 kDa EndoG of human, mouse, and rat origins. Reactivity to other species has not been tested.

Other Notes

Small volumes of anti-EndoG antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EndoG purchase

MBS840898 is an antibody targeting EndoG. Also known as EndoG Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for EndoG. It may not necessarily be applicable to this product.

NCBI GI #

53759134

NCBI GeneID

2021

NCBI Accession #

NP_004426.2

UniProt Secondary Accession #

Q5T281; Q9BSP2

UniProt Related Accession #

Q14249

GenBank Nucleotide #

NM_004435.2

NCBI Official Full Name

endonuclease G, mitochondrial

NCBI Official Synonym Full Names

endonuclease G

NCBI Official Symbol

ENDOG

UniProt Protein Name

Endonuclease G, mitochondrial

Protein Family

Endonuclease

UniProt Gene Name

ENDOG

UniProt Synonym Gene Names

Endo G

UniProt Entry Name

NUCG_HUMAN

NCBI Protein Information

endonuclease G, mitochondrial

NCBI Summary for EndoG

The protein encoded by this gene is a nuclear encoded endonuclease that is localized in the mitochondrion. The encoded protein is widely distributed among animals and cleaves DNA at GC tracts. This protein is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA. [provided by RefSeq, Jul 2008]

UniProt Comments for EndoG

ENDOG: Cleaves DNA at double-stranded (DG)n.(DC)n and at single-stranded (DC)n tracts. In addition to deoxyribonuclease activities, also has ribonuclease (RNase) and RNase H activities. Capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA. Homodimer. Belongs to the DNA/RNA non-specific endonuclease family.

Protein type: EC 3.1.30.-; Mitochondrial; Ribonuclease; Apoptosis; Deoxyribonuclease

Chromosomal Location of Human Ortholog: 9q34.1

Cellular Component: mitochondrion

Molecular Function: protein binding; nuclease activity; nucleic acid binding; metal ion binding; endonuclease activity

Biological Process: response to antibiotic; positive regulation of apoptosis; in utero embryonic development; DNA fragmentation during apoptosis; DNA recombination

Research Articles on EndoG

1. Data suggest that endonuclease G (EndoG) inhibitors have the potential to be utilized for amelioration of cell injuries in which participation of EndoG is essential.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.