Rabbit Anti-CYCT Polyclonal Antibody – Cat# MBS841048

Cytochrome C (CYCT) Antibody – Rabbit Polyclonal

Name: CYCT Antibody; Polyclonal; Rabbit; MyBioSource
Brand: MyBioSource
SKU: MBS841048
Price: 215 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS841048

Background: Cytochrome c (m.w. 12,500) is an electron transport protein from mitochondria. It is released from mitochondria to cytoplasm during the early stages of apoptosis, prior to caspase activation, DNA fragmentation, and loss of membrane potential. The… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

Cytochrome c (Cyct), Polyclonal Antibody

Full Product Name

Cytochrome c Antibody

Product Gene Name

anti-Cyct antibody

Product Synonym Names

CYCS; CYC

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human, Mouse, Rat, Bovine, Chicken, Dog

Form/Format

100 ug (0.2 mg/ml) affinity purified rabbit anti-Cytochrome c polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Appearance: Colorless liquid

Purity/Purification

Affinity purified

Concentration

0.2 mg/ml (lot specific)

Preparation and Storage

At -20 degree C
Shelf Life: 12 months

Molecular Weight

11,743 Da

Immunogen

Synthetic peptide corresponding to residues surrounding amino acid 70 of rat cytochrome c

Handling

The antibody solution should be gently mixed before use.

Applications Tested/Suitable for anti-Cyct antibody

Western Blot (WB), Immunoprecipitation (IP)

Application Notes for anti-Cyct antibody

Western blotting (0.5-4 ug/ml) and immunoprecipitation (10-20 ug/ml). The cytochrome c antibody detects the 12.6 kDa cytochrome c from human, mouse, and rat samples. Jurkat cell lysate, NIH3T3 cell lysate, and rat kidney tissue lysate can be used as positive controls.

Other Notes

Small volumes of anti-Cyct antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Cyct purchase

MBS841048 is an antibody targeting Cyct. Also known as Cytochrome c Antibody. Reactive in Human, Mouse, Rat, Bovine, Chicken, Dog. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Cyct. It may not necessarily be applicable to this product.

NCBI GI #

6978727

NCBI GeneID

25310

NCBI Accession #

NP_036972

UniProt Related Accession #

P10715

GenBank Nucleotide #

NM_012840.3

NCBI Official Full Name

cytochrome c, testis-specific

NCBI Official Synonym Full Names

cytochrome c, testis

NCBI Official Symbol

Cyct

NCBI Official Synonym Symbols

CYCTA

UniProt Protein Name

Cytochrome c, testis-specific

Protein Family

Cyclin

UniProt Gene Name

Cyct

UniProt Entry Name

CYC2_RAT

NCBI Protein Information

cytochrome c, testis-specific

NCBI Summary for Cyct

testis specific cytochrome c; expressed during spermatogenesis [RGD, Feb 2006]

UniProt Comments for Cyct

CYCT: Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases. Belongs to the cytochrome c family

Cellular Component: mitochondrial intermembrane space

Molecular Function: electron carrier activity; metal ion binding; iron ion binding; heme binding; 3',5'-cyclic-nucleotide phosphodiesterase activity

Biological Process: apoptosis; metabolic process; signal transduction

Research Articles on Cyct

1. These findings suggest that heat stress of testes triggers the release of cytochrome c from mitochondria in spermatogenic cells, leading to the activation of an apoptotic pathway.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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