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DNMT2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS841219

Polyclonal Antibody to detect DNMT2 in mouse samples. Background: Dnmt2 belongs to the family of DNA cytosine methyltransferases. Dnmt2, however, lacks the large N-terminal regulatory domain common to other eukaryotic methyltransferases and does not methylate… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
DNMT2, Polyclonal Antibody
Full Product Name
DNMT2 polyclonal antibody
Product Gene Name
Product Synonym Names
TRDMT1; M.HsaIIP; PuMet; RNMT1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse
Form/Format
In PBS with 0.05% (W/V) sodium azide.
Appearance: Colorless liquid
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Molecular Weight
12,087 Da
OMIM
602478
3D Structure
ModBase 3D Structure for O14717
Immunogen
Polyclonal antibody raised in rabbit against mouse Dnmt2 (DNA methyltransferase 2), using a KLH conjugated synthetic peptide containing an amino acid sequence from the N-terminal part of the protein.
Positive Control
Western blot: NIH3T3 and embryonic stem cells, ELISA: Peptides.
Handling
The antibody solution should be gently mixed before use.
Applications Tested/Suitable for anti-DNMT2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-DNMT2 antibody
Western Blot: 1:1000, ELISA: 1:100 - 1:1000

Testing Data of anti-DNMT2 antibody

Rabbit Anti-DNMT2 Polyclonal Antibody – Cat# MBS841219

Other Notes

Small volumes of anti-DNMT2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DNMT2 purchase

MBS841219 is an antibody targeting DNMT2. Also known as DNMT2 polyclonal antibody. Reactive in Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for DNMT2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
tRNA (cytosine(38)-C(5))-methyltransferase
NCBI Official Synonym Full Names
tRNA aspartic acid methyltransferase 1
NCBI Official Symbol
TRDMT1
NCBI Official Synonym Symbols
DMNT2; DNMT2; PUMET; RNMT1; MHSAIIP
UniProt Protein Name
tRNA (cytosine(38)-C(5))-methyltransferase
UniProt Synonym Protein Names
DNA (cytosine-5)-methyltransferase-like protein 2; Dnmt2; DNA methyltransferase homolog HsaIIP; DNA MTase homolog HsaIIP; M.HsaIIP; PuMet
Protein Family
UniProt Gene Name
TRDMT1
UniProt Synonym Gene Names
DNMT2; Dnmt2; DNA MTase homolog HsaIIP; M.HsaIIP
UniProt Entry Name
TRDMT_HUMAN
NCBI Protein Information
tRNA (cytosine(38)-C(5))-methyltransferase

NCBI Summary for DNMT2

This gene encodes a protein responsible for the methylation of aspartic acid transfer RNA, specifically at the cytosine-38 residue in the anticodon loop. This enzyme also possesses residual DNA-(cytosine-C5) methyltransferase activity. While similar in sequence and structure to DNA cytosine methyltransferases, this gene is distinct and highly conserved in its function among taxa. [provided by RefSeq, Jun 2010]
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UniProt Comments for DNMT2

TRDMT1: Specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp). Belongs to the C5-methyltransferase family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - cysteine and methionine; Methyltransferase; Methyltransferase, tRNA; EC 2.1.1.204

Chromosomal Location of Human Ortholog: 10p15.1

Cellular Component: cytoplasm; nucleus

Molecular Function: DNA (cytosine-5-)-methyltransferase activity; DNA binding; RNA binding; tRNA methyltransferase activity

Biological Process: response to amphetamine; tRNA methylation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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