Rabbit Anti-SPHK2 Polyclonal Antibody – Cat# MBS841254

Sphingosine Kinase 2 (SPK2) (SPHK2) Antibody – Rabbit Polyclonal

Name: SPHK2 Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS841254
Price: 215 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS841254

Background: Sphingosine Kinase (SphK) is a conserved lipid kinase that catalyzes the phosphorylation of sphingolipid sphingosine to sphingosine-1-phosphate (SIP). SIP receptors coupled to cell surface G protein to regulate cell growth, survival, motility and… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

Sphingosine Kinase 2 (SPK2) (Sphk2), Polyclonal Antibody

Full Product Name

Sphingosine Kinase 2 (SPK2) Antibody

Product Gene Name

anti-Sphk2 antibody

Product Synonym Names

SPK2; SPK-2; SPK 2; sphk; SK 2; SK2

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Form/Format

100 ug (0.5mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.
Appearance: Colorless liquid

Purity/Purification

Affinity purified

Concentration

0.5 mg/ml (lot specific)

Preparation and Storage

At -20 degree C
Shelf Life: 12 months

Molecular Weight

65,523 Da

Immunogen

Synthetic peptide corresponding to residues surrounding amino acid 603 of rat Sphingosine Kinase 2

Handling

The antibody solution should be gently mixed before use.

Applications Tested/Suitable for anti-Sphk2 antibody

Western Blot (WB)

Application Notes for anti-Sphk2 antibody

Western blotting (0.5-4 ug/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~69 kDa bands from samples of human, mouse and rat origins. Reactivity to other species has not been tested.

Other Notes

Small volumes of anti-Sphk2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Sphk2 purchase

MBS841254 is an antibody targeting Sphk2. Also known as Sphingosine Kinase 2 (SPK2) Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Sphk2. It may not necessarily be applicable to this product.

NCBI GI #

50927705

NCBI GeneID

308589

NCBI Accession #

AAH79120

NCBI Official Full Name

Sphingosine kinase 2

NCBI Official Synonym Full Names

sphingosine kinase 2

NCBI Official Symbol

Sphk2

UniProt Protein Name

Protein Sphk2

UniProt Synonym Protein Names

Protein Sphk2

Protein Family

Sphingosine kinase

UniProt Gene Name

Sphk2

UniProt Entry Name

Q6AYB2_RAT

NCBI Protein Information

sphingosine kinase 2

UniProt Comments for Sphk2

SPHK2: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro- dihydrosphingosine, D-erythro-sphingosine and L-threo- dihydrosphingosine. Binds phosphoinositides. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - sphingolipid; Kinase, lipid; EC 2.7.1.91

Cellular Component: membrane; lysosomal membrane; cytosol

Molecular Function: D-erythro-sphingosine kinase activity; diacylglycerol kinase activity; NAD+ kinase activity

Biological Process: blood vessel development; positive regulation of cell proliferation; protein kinase C activation; female pregnancy; brain development; sphingosine metabolic process; phosphorylation

Research Articles on Sphk2

1. Glucose-supported beta-cell growth appears to be in part mediated by SPHK activity.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.