HGF Active Protein

Name: HGF Active Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS844133
Price: 305 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS844133

Background: HGF is a mesenchymally derived potent mitogen for mature parenchymal hepatocyte cells and acts as a growth factor for a broad spectrum of tissues and cell types. HGF signals through a transmembrane tyrosine kinase receptor known as MET. Activities… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

HGF, Active Protein

Full Product Name

HGF, murine recombinant

Product Gene Name

HGF active protein

Product Synonym Names

Scatter Factor (SF); Hepatopoietin (HPTA)

Sequence

Alpha chain: QKKRRNTLHE FKKSAKTTLT KEDPLLKIKT KKVNSADECA NRCIRNRGFT FTCKAFVFDK SRKRCYWYPF NSMSSGVKKG FGHEFDLYEN KDYIRNCIIG KGGSYKGTVS ITKSGIKCQP WNSMIPHEHS FLPSSYRGKD LQENYCRNPR GEEGGPWCFT SNPEVRYEVC DIPQCSEVEC MTCNGESYRG PMDHTESGKT CQRWDQQTPH RHKFLPERYP DKGFDDNYCR NPDGKPRPWC YTLDPDTPWE YCAIKTCAHS AVNETDVPME TTECIQGQGE GYRGTSNTIW NGIPCQRWDS QYPHKHDITP ENFKCKDLRE NYCRNPDGAE SPWCFTTDPN IRVGYCSQIP KCDVSSGQDC YRGNGKNYMG NLSKTRSGLT CSMWDKNMED LHRHIFWEPD ASKLNKNYCR NPDDDAHGPW CYTGNPLIPW DYCPISRCEG DTTPTIVNLD HPVISCAKTK QLR Beta chain: VVNGIPTQTT VGWMVSLKYR NKHICGGSLI KESWVLTARQ CFPARNKDLK DYEAWLGIHD VHERGEEKRK QILNISQLVY GPEGSDLVLL KLARPAILDN FVSTIDLPSY GCTIPEKTTC SIYGWGYTGL INADGLLRVA HLYIMGNEKC SQHHQGKVTL NESELCAGAE KIGSGPCEGD YGGPLICEQH KMRMVLGVIV PGRGCAIPNR PGIFVRVAYY AKWIHKVILT YKL

Host

(BTI-Tn-5B1-4) Hi-5 Insect Cells

Form/Format

Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Tris, pH 7.2, 100 mM L-Arginine, 200 mM NaCl.
Appearance: Lyophilized powder

Purity/Purification

>=95%

Preparation and Storage

At -20 degree C
Shelf Life: 12 months

Molecular Weight

~85 kDa

3D Structure

ModBase 3D Structure for Q08048

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Solubility/Reconstitution Instructions

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20 degree C to -80 degree C.

Endotoxin Level

< 0.1 ng/mug of protein (<1EU/mug).

Activity (Specifications/test method)

Determined by the dose-dependent stimulation of the proliferation of mouse IMCD3 cells using a concentration range of 10-20 ng/ml.

Biological Activity

Determined by the dose-dependent stimulation of the proliferation of mouse IMCD3 cells using a concentration range of 10-20 ng/ml.

Handling

Centrifuge the vial prior to opening.

Product Categories/Family for HGF active protein

Cytokines; Growth Factors & Hormones; HGFsCytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A-Z) Proteins and Enzymes; Cytokines; Growth Factors & Hormones; HGFsProteins and Enzymes; Cytokines; Growth Factors & Hormones; Cytokines & Growth

Applications Tested/Suitable for HGF active protein

SDS-PAGE; HPLC

Other Notes

Small volumes of HGF active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HGF purchase

MBS844133 is an active protein targeting HGF. Also known as HGF, murine recombinant. Host: (BTI-Tn-5B1-4) Hi-5 Insect Cells.

Additional Details

NCBI/Uniprot data below describe general gene information for HGF. It may not necessarily be applicable to this product.

NCBI GI #

575501559

NCBI GeneID

15234

NCBI Accession #

NP_001276387.1

UniProt Primary Accession #

Q08048

UniProt Secondary Accession #

O55027; Q53WS5; Q61662; Q64007; Q6LBE6

UniProt Related Accession #

Q08048

GenBank Nucleotide #

NM_001289458.1

NCBI Official Full Name

hepatocyte growth factor isoform 1 preproprotein

NCBI Official Synonym Full Names

hepatocyte growth factor

NCBI Official Symbol

Hgf

NCBI Official Synonym Symbols

SF; NK1; NK2; HGF/SF; SF/HGF; C230052L06Rik

UniProt Protein Name

Hepatocyte growth factor

UniProt Synonym Protein Names

Hepatopoietin-A; Scatter factor; SF

Protein Family

Hepatocyte growth factor

UniProt Gene Name

Hgf

UniProt Synonym Gene Names

UniProt Entry Name

HGF_MOUSE

NCBI Protein Information

hepatocyte growth factor

UniProt Comments for HGF

HGF: HGF is a potent mitogen for mature parenchymal hepatocyte cells, seems to be an hepatotrophic factor, and acts as growth factor for a broad spectrum of tissues and cell types. It has no detectable protease activity. Activating ligand for the receptor tyrosine kinase MET by binding and promoting its dimerization. Defects in HGF are the cause of deafness autosomal recessive type 39 (DFNB39). A form of profound prelingual sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Belongs to the peptidase S1 family. Plasminogen subfamily. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Hormone; Cell development/differentiation

Cellular Component: extracellular space; membrane

Molecular Function: identical protein binding; growth factor activity; protein heterodimerization activity; serine-type endopeptidase activity; catalytic activity; chemoattractant activity

Biological Process: positive regulation of myelination; activation of MAPK activity; myoblast proliferation; cell morphogenesis; hepatocyte growth factor receptor signaling pathway; negative regulation of caspase activity; liver development; proteolysis; positive regulation of phosphoinositide 3-kinase cascade; cell proliferation; positive regulation of angiogenesis; positive regulation of peptidyl-tyrosine phosphorylation; positive chemotaxis; positive regulation of cell migration; negative regulation of apoptosis

Research Articles on HGF

1. HGF/c-MET signaling promotes tumor formation after tissue injury in a mouse model of primary soft-tissue sarcoma

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with HGF active protein

Reference Product	PubMed Publications
Met active protein	>2810 publications with HGF and Met
Egfr active protein	>261 publications with HGF and Egfr
Src active protein	>152 publications with HGF and Src
Grb2 active protein	>72 publications with HGF and Grb2
Akt1 active protein	>60 publications with HGF and Akt1
Erbb2 active protein	>53 publications with HGF and Erbb2
Mmp2 active protein	>35 publications with HGF and Mmp2
Kdr active protein	>28 publications with HGF and Kdr
Sdc1 active protein	>10 publications with HGF and Sdc1
Tek active protein	>6 publications with HGF and Tek

Pathways associated with HGF active protein

Products by Pathway	Pathway Diagram
Cytokine Signaling In Immune System Pathway antibodies	Cytokine Signaling In Immune System Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Interleukin-7 Signaling Pathway antibodies	Interleukin-7 Signaling Pathway Diagram
Malaria Pathway antibodies	Malaria Pathway Diagram

Diseases associated with HGF active protein

Disease Name	Pubmed Publications
Liver Diseases Antibodies	>785 publications with HGF and Liver Diseases
Vascular Diseases Antibodies	>624 publications with HGF and Vascular Diseases
Cardiovascular Diseases Antibodies	>614 publications with HGF and Cardiovascular Diseases
Necrosis Antibodies	>476 publications with HGF and Necrosis
Fibrosis Antibodies	>461 publications with HGF and Fibrosis
Inflammation Antibodies	>443 publications with HGF and Inflammation
Liver Neoplasms Antibodies	>441 publications with HGF and Liver Neoplasms
Neoplasm Invasiveness Antibodies	>436 publications with HGF and Neoplasm Invasiveness
Neoplasms, Experimental Antibodies	>315 publications with HGF and Neoplasms, Experimental
Kidney Diseases Antibodies	>309 publications with HGF and Kidney Diseases

Organs/Tissues associated with HGF active protein

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>1720 publications with HGF and Liver
Lung Antibodies	>720 publications with HGF and Lung
Embryonic Tissue Antibodies	>382 publications with HGF and Embryonic Tissue
Brain Antibodies	>362 publications with HGF and Brain
Heart Antibodies	>317 publications with HGF and Heart
Testis Antibodies	>27 publications with HGF and Testis
Thymus Antibodies	>20 publications with HGF and Thymus
Vagina Antibodies	>1 publications with HGF and Vagina