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MIP-1beta (CCL4) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS844146

Background: Human macrophage inflammatory protein-1 beta (MIP-1 beta) is a 7.6 kDa protein containing 69 amino acid residues. It plays an important role in the inflammatory response of blood monocytes and tissue macrophages. More details

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Product Overview

Product Name
MIP-1beta (CCL4), Active Protein
Full Product Name
MIP-1beta, human recombinant
Product Gene Name
CCL4 active protein
Product Synonym Names
Macrophage Inflammatory Protein-1 beta; CCL4; ACT-2; G-26 T-lymphocyte-secreted protein; HC21; Lymphocyte activation gene 1 protein; PAT 744; Protein H400; SIS-gamma; Small-inducible cytokine A4; T-cell activation protein 2
Host
E Coli
Form/Format
Sterile filtered and lyophilized with no additives
Appearance: Lyophilized protein
Purity/Purification
>=99%
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Molecular Weight
7.6 kDa
OMIM
182284
3D Structure
ModBase 3D Structure for P13236
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Solubility/Reconstitution Instructions
Reconstitute in H?O to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers
Endotoxin Level
<0.1 ng/mug
Biological Activity
Determined by its ability to chemoattract human blood monocytes using a concentration of 5.0-20 ng/ml.
Results
5.0-20 ng/ml
Handling
Centrifuge the vial prior to opening.

Product Categories/Family for CCL4 active protein

Cytokines; Growth Factors & Hormones; ChemokinesCytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A-Z) Proteins and Enzymes; Cytokines; Growth Factors & Hormones; Chemokines Proteins and Enzymes; Cytokines; Growth Factors & Hormones; Cytok
Applications Tested/Suitable for CCL4 active protein
SDS-PAGE; HPLC

Other Notes

Small volumes of CCL4 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CCL4 purchase

MBS844146 is an active protein targeting CCL4. Also known as MIP-1beta, human recombinant. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for CCL4. It may not necessarily be applicable to this product.

NCBI GI #
4506845
NCBI GeneID
6351
NCBI Accession #
NP_002975.1
UniProt Primary Accession #
P13236
UniProt Secondary Accession #
P22617; Q13704; Q3SXL8; Q6FGI8
UniProt Related Accession #
P13236
GenBank Nucleotide #
NM_002984.3
NCBI Official Full Name
C-C motif chemokine 4
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 4
NCBI Official Symbol
CCL4
NCBI Official Synonym Symbols
ACT2; G-26; HC21; LAG1; LAG-1; MIP1B; SCYA2; SCYA4; MIP1B1; AT744.1; MIP-1-beta
UniProt Protein Name
C-C motif chemokine 4
UniProt Synonym Protein Names
G-26 T-lymphocyte-secreted protein; HC21; Lymphocyte activation gene 1 protein; LAG-1
Protein Family
C-C motif chemokine
UniProt Gene Name
CCL4
UniProt Synonym Gene Names
LAG1; MIP1B; SCYA4; LAG-1; MIP-1-beta; ACT-2
UniProt Entry Name
CCL4_HUMAN
NCBI Protein Information
C-C motif chemokine 4

NCBI Summary for CCL4

The protein encoded by this gene is a mitogen-inducible monokine and is one of the major HIV-suppressive factors produced by CD8+ T-cells. The encoded protein is secreted and has chemokinetic and inflammatory functions. [provided by RefSeq, Dec 2012]

UniProt Comments for CCL4

CCL4: Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5- mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted; Secreted, signal peptide; Chemokine; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: extracellular space; extracellular region

Molecular Function: identical protein binding; protein binding; CCR1 chemokine receptor binding; chemokine activity; cytokine activity; CCR5 chemokine receptor binding

Biological Process: cell-cell signaling; response to toxin; response to virus; establishment and/or maintenance of cell polarity; immune response; positive regulation of calcium-mediated signaling; inflammatory response; cell motility; cell adhesion; signal transduction; positive regulation of calcium ion transport
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Research Articles on CCL4

1. the secretion of IL-1alpha, IL-1beta, IL-12b, and CCL4 occurs during gelatinase degranulation, a process controlled by STX3.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CCL4 active protein

 Reference Product  PubMed Publications
 CCL3 active protein  >1647 publications with CCL4 and CCL3
 CCR5 active protein  >605 publications with CCL4 and CCR5
 CCR1 active protein  >200 publications with CCL4 and CCR1
 CCR2 active protein  >111 publications with CCL4 and CCR2
 CXCR3 active protein  >76 publications with CCL4 and CXCR3
 CCR3 active protein  >73 publications with CCL4 and CCR3
 CCR7 active protein  >53 publications with CCL4 and CCR7
 CCL17 active protein  >50 publications with CCL4 and CCL17
 IDE active protein  >5 publications with CCL4 and IDE
 CCBP2 active protein  >1 publications with CCL4 and CCBP2

Pathways associated with CCL4 active protein

 Products by Pathway  Pathway Diagram
 Chemokine Receptors Bind Chemokines Pathway antibodies  Chemokine Receptors Bind Chemokines Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 IL12-mediated Signaling Events Pathway antibodies  IL12-mediated Signaling Events Pathway Diagram

Diseases associated with CCL4 active protein

 Disease Name  Pubmed Publications
 Immune System Diseases Antibodies  >701 publications with CCL4 and Immune System Diseases
 Disease Progression Antibodies  >244 publications with CCL4 and Disease Progression
 Protein Deficiency Antibodies  >166 publications with CCL4 and Protein Deficiency
 Bone Diseases Antibodies  >108 publications with CCL4 and Bone Diseases
 Hypertension, Portal Antibodies  >95 publications with CCL4 and Hypertension, Portal
 Disease Susceptibility Antibodies  >78 publications with CCL4 and Disease Susceptibility
 Eye Diseases Antibodies  >76 publications with CCL4 and Eye Diseases
 Pneumonia Antibodies  >70 publications with CCL4 and Pneumonia
 Ischemia Antibodies  >63 publications with CCL4 and Ischemia
 Diabetes Mellitus Antibodies  >53 publications with CCL4 and Diabetes Mellitus

Organs/Tissues associated with CCL4 active protein

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >4533 publications with CCL4 and Liver
 Blood Antibodies  >3344 publications with CCL4 and Blood
 Lung Antibodies  >411 publications with CCL4 and Lung
 Bone Marrow Antibodies  >304 publications with CCL4 and Bone Marrow
 Kidney Antibodies  >296 publications with CCL4 and Kidney
 Intestine Antibodies  >121 publications with CCL4 and Intestine
 Lymph Antibodies  >112 publications with CCL4 and Lymph
 Parathyroid Antibodies  >14 publications with CCL4 and Parathyroid
 Prostate Antibodies  >13 publications with CCL4 and Prostate
 Adrenal Gland Antibodies  >7 publications with CCL4 and Adrenal Gland
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