PLGF/PIGF2/PGF (PLGF) Active Protein

Name: PLGF Active Protein; PLGF/PIGF2/PGF; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS844157
Price: 545 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS844157

Background: Placental growth factor (PGF) also known as vascular endothelial growth factor-related protein, PLGF and PlGF2, is a member of the VEGF (vascular endothelial growth factor) sub-family - a key molecule in angiogenesis and vasculogenesis, in… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

PLGF/PIGF2/PGF, Active Protein

Full Product Name

Human CellExp PLGF / PIGF2 / PGF, human recombinant

Product Gene Name

PLGF active protein

Product Synonym Names

PGF; PLGF; PlGF2; PlGF; PGFL; SHGC-10760

Host

HEK 293 cells

Form/Format

Lyophilized from 0.22 mum filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Appearance: Lyophilized powder

Purity/Purification

>=95%

Preparation and Storage

At -20 degree C
Shelf Life: 12 months

Molecular Weight

The protein is fused with 6×His tag at the C-terminus, has a calculated MW of 20.2 kDa. The predicted N-terminus is Leu 19. DTT-reduced Protein migrates as 28-33 kDa due to glycosylation.

OMIM

601121

3D Structure

ModBase 3D Structure for P49763

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Solubility/Reconstitution Instructions

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.

Endotoxin Level

< 0.1 ng/mug of protein (<1EU/mug).

Activity (Specifications/test method)

Measured in a cell proliferation assay using MDA - MB - 231 human breast cancer cells. The ED50 for this effect is typically 1 - 10 ug/ml.

Biological Activity

Measured in a cell proliferation assay using MDA - MB - 231 human breast cancer cells. The ED50 for this effect is typically 1 - 10 ug/ml.

Handling

Centrifuge the vial prior to opening.

Product Categories/Family for PLGF active protein

Cytokines; Growth Factors & Hormones; Human cell expressed recombinant proteinsProteins and Enzymes; Human cell expressed recombinant proteins Cytokines; Growth Factors & Hormones; VEGFsCytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A-Z

Applications Tested/Suitable for PLGF active protein

SDS-PAGE; HPLC

Other Notes

Small volumes of PLGF active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PLGF purchase

MBS844157 is an active protein targeting PLGF. Also known as Human CellExp PLGF / PIGF2 / PGF, human recombinant. Host: HEK 293 cells.

Additional Details

NCBI/Uniprot data below describe general gene information for PLGF. It may not necessarily be applicable to this product.

NCBI GI #

333033785

NCBI GeneID

5228

NCBI Accession #

NP_001193941.1

UniProt Primary Accession #

P49763

UniProt Secondary Accession #

Q07101; Q9BV78; Q9Y6S8

UniProt Related Accession #

P49763

GenBank Nucleotide #

NM_001207012.1

NCBI Official Full Name

placenta growth factor isoform 2

NCBI Official Synonym Full Names

placental growth factor

NCBI Official Symbol

PGF

NCBI Official Synonym Symbols

PGFL; PLGF; PlGF-2; D12S1900; SHGC-10760

UniProt Protein Name

Placenta growth factor

UniProt Gene Name

PGF

UniProt Synonym Gene Names

PGFL; PLGF; PlGF

UniProt Entry Name

PLGF_HUMAN

NCBI Protein Information

placenta growth factor

NCBI Summary for PLGF

This gene encodes a growth factor found in placenta which is homologous to vascular endothelial growth factor. Alternatively spliced transcripts encoding different isoforms have been found for this gene.[provided by RefSeq, Jun 2011]

UniProt Comments for PLGF

PGF: Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth. Belongs to the PDGF/VEGF growth factor family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 14q24.3

Cellular Component: extracellular space; membrane; extracellular region

Molecular Function: heparin binding; protein binding; protein homodimerization activity; growth factor activity; protein heterodimerization activity

Biological Process: response to drug; organ regeneration; female pregnancy; signal transduction; cellular response to hormone stimulus; positive regulation of angiogenesis; cell-cell signaling; ureteric bud branching; positive regulation of cell division; positive regulation of cell proliferation; response to hypoxia; positive regulation of endothelial cell proliferation; sprouting angiogenesis; cell differentiation; vascular endothelial growth factor receptor signaling pathway

Research Articles on PLGF

1. Maternal PGF concentrations were lower in twin than singleton pregnancies at week 35 only Placental weight appeared to be inversely correlated with maternal sFlt-1/PGF ratio at the end of pregnancy in both twins and singletons.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PLGF active protein

Reference Product	PubMed Publications
VEGFA active protein	>26 publications with PLGF and VEGFA
FLT1 active protein	>21 publications with PLGF and FLT1
KDR active protein	>14 publications with PLGF and KDR
VEGFC active protein	>8 publications with PLGF and VEGFC
VEGFB active protein	>5 publications with PLGF and VEGFB
NRP1 active protein	>5 publications with PLGF and NRP1
TEK active protein	>4 publications with PLGF and TEK
FIGF active protein	>3 publications with PLGF and FIGF
FLT4 active protein	>3 publications with PLGF and FLT4
PIK3CA active protein	>1 publications with PLGF and PIK3CA

Diseases associated with PLGF active protein

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>395 publications with PLGF and Cardiovascular Diseases
Inflammation Antibodies	>296 publications with PLGF and Inflammation
Hypertension Antibodies	>198 publications with PLGF and Hypertension
Disease Models, Animal Antibodies	>162 publications with PLGF and Disease Models, Animal
Necrosis Antibodies	>159 publications with PLGF and Necrosis
Heart Diseases Antibodies	>132 publications with PLGF and Heart Diseases
Lung Diseases Antibodies	>131 publications with PLGF and Lung Diseases
Atherosclerosis Antibodies	>93 publications with PLGF and Atherosclerosis
Brain Diseases Antibodies	>73 publications with PLGF and Brain Diseases
Kidney Diseases Antibodies	>71 publications with PLGF and Kidney Diseases

Organs/Tissues associated with PLGF active protein

Organ/Tissue Name	Pubmed Publications
Ovary Antibodies	>774 publications with PLGF and Ovary
Uterus Antibodies	>733 publications with PLGF and Uterus
Muscle Antibodies	>508 publications with PLGF and Muscle
Heart Antibodies	>253 publications with PLGF and Heart
Lung Antibodies	>241 publications with PLGF and Lung
Kidney Antibodies	>215 publications with PLGF and Kidney
Placenta Antibodies	>215 publications with PLGF and Placenta
Embryonic Tissue Antibodies	>200 publications with PLGF and Embryonic Tissue
Brain Antibodies	>196 publications with PLGF and Brain
Liver Antibodies	>139 publications with PLGF and Liver