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Persephin (PSPN) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS844158

Background: Persephin is a disulfide-linked homodimer neurotrophic factor structurally related to GDNF, Artemin, and Neurturin. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. Persephin signals… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
Persephin (PSPN), Active Protein
Full Product Name
Persephin, human recombinant
Product Gene Name
PSPN active protein
Product Synonym Names
PSP; PSPN
Sequence
RALSGPCQLW SLTLSVAELG LGYASEEKVI FRYCAGSCPR GARTQHGLAL ARLQGQGRAH GGPCCRPTRY TDVAFLDDRH RWQRLPQLSA AACGCGG
Host
E Coli
Form/Format
Sterile filtered through a 0.2 micron filter. Lyophilized from 0.3 x PBS, pH 7.2
Appearance: Lyophilized powder
Purity/Purification
>=98%
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Molecular Weight
10.4 kDa
OMIM
602921
3D Structure
ModBase 3D Structure for O60542
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Solubility/Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in 5 mM Sodium Phosphate, pH 7.5 to a concentration of <= 0.5 mg/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20 degree C to -80 degree C.
Endotoxin Level
< 0.1 ng/mug of protein (<1EU/mug).
Activity (Specifications/test method)
Human Persephin induces RET phosphorylation using a concentration range of 0.1-1.0 ng/ml. Human Persephin binds to mammalian GFRalpha4 with the Kd of 100pM. Other members of the GDNF family (Artemin, GDNF and Neurturin) do not bind to mammalian GFRalpha4.
Biological Activity
Human Persephin induces RET phosphorylation using a concentration range of 0.1-1.0 ng/ml. Human Persephin binds to mammalian GFRalpha4 with the Kd of 100pM. Other members of the GDNF family (Artemin, GDNF and Neurturin) do not bind to mammalian GFRalpha4.
Handling
Centrifuge the vial prior to opening.

Product Categories/Family for PSPN active protein

Cytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A-Z) Proteins and Enzymes; Cytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A-Z) Proteins and Enzymes; Animal-Free Origin (AOF) Recombinant Proteins & EnzymesProteins and E
Applications Tested/Suitable for PSPN active protein
SDS-PAGE; HPLC

Other Notes

Small volumes of PSPN active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PSPN purchase

MBS844158 is an active protein targeting PSPN. Also known as Persephin, human recombinant. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for PSPN. It may not necessarily be applicable to this product.

NCBI GI #
4758974
NCBI GeneID
5623
NCBI Accession #
NP_004149.1
UniProt Primary Accession #
O60542
UniProt Related Accession #
O60542
GenBank Nucleotide #
NM_004158.2
NCBI Official Full Name
persephin
NCBI Official Synonym Full Names
persephin
NCBI Official Symbol
PSPN
NCBI Official Synonym Symbols
PSP
UniProt Protein Name
Persephin
Protein Family
Persephin
UniProt Gene Name
PSPN
UniProt Synonym Gene Names
PSP
UniProt Entry Name
PSPN_HUMAN
NCBI Protein Information
persephin

NCBI Summary for PSPN

The protein encoded by this gene is a neurotrophic factor, belonging to the GDNF family. Neurotrophic factors are important for the proper development and maintenance of the nervous system. These factors promote neuronal survival and can prevent the neuronal degeneration associated with injury, toxin exposure, or neurodegenerative disease. The encoded protein has amino acid similarity to its other family members, glial cell line-derived neurotrophic factor and neurturin. This gene product promotes the survival of ventral midbrain dopaminergic neurons in culture and prevents their degeneration after 6-hydroxydopamine treatment in vivo. [provided by RefSeq, Jul 2008]
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UniProt Comments for PSPN

PSPN: Exhibits neurotrophic activity on mesencephalic dopaminergic and motor neurons. Belongs to the TGF-beta family. GDNF subfamily.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: extracellular space

Molecular Function: growth factor activity; receptor binding

Biological Process: axon guidance; nervous system development; central nervous system development; ureteric bud branching
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Research Articles on PSPN

1. Results identify persephin, a GDNF family member, as a novel ligand for GFRalpha1/RET receptor complex.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PSPN active protein

 Reference Product  PubMed Publications
 GDNF active protein  >20 publications with PSPN and GDNF
 RET active protein  >13 publications with PSPN and RET
 NRTN active protein  >12 publications with PSPN and NRTN
 ARTN active protein  >10 publications with PSPN and ARTN
 GFRA1 active protein  >5 publications with PSPN and GFRA1
 GFRA2 active protein  >4 publications with PSPN and GFRA2
 GFRA4 active protein  >2 publications with PSPN and GFRA4
 GFRA3 active protein  >1 publications with PSPN and GFRA3
 NCAM1 active protein  >1 publications with PSPN and NCAM1

Diseases associated with PSPN active protein

 Disease Name  Pubmed Publications
 Neurodegenerative Diseases Antibodies  >1 publications with PSPN and Neurodegenerative Diseases

Organs/Tissues associated with PSPN active protein

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >7 publications with PSPN and Brain
 Lung Antibodies  >1 publications with PSPN and Lung
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