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EPOR/Erythropoietin Receptor (EPOR) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS844166

Background: Erythropoietin (EPO) is the major glycoprotein hormone regulator of mammalian erythropoiesis, and is produced by kidney and liver in an oxygen-dependent manner. The biological effects of EPO are mediated by the specific erythropoietin receptor… More details

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Product Overview

Product Name
EPOR/Erythropoietin Receptor, Active Protein
Full Product Name
Human CellExp EPOR / Erythropoietin Receptor, human recombinant
Product Gene Name
EPOR active protein
Product Synonym Names
EPOR; Erythropoietin Receptor.
Host
HEK293 cells
Form/Format
Lyophilized from 0.22 mum filtered solution in 50 mM tris, 100 mM glycine, pH 7.0. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Appearance: Lyophilized
Purity/Purification
>=92%
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Molecular Weight
This protein is fused with Fc fragment of human IgG1 at the C-terminus, has a calculated MW of 50.1 kDa. The predicted N-terminus is Ala 25. DTT-reduced Protein migrates as 55-60 kDa due to glycosylation.
OMIM
133100
3D Structure
ModBase 3D Structure for P19235
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Solubility/Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.
Endotoxin Level
<1 EU/mug by LAL method
Biological Activity
Measured by its ability to inhibit Epo-dependent proliferation of TF?1 human erythroleukemic cells. The ED50 for this effect is typically 2 - 6 ng/ml in the presence of 0.2 U/ml of rhEpo-Fc.
Results
Measured by its ability to inhibit Epo-dependent proliferation of TF?1 human erythroleukemic cells. The ED50 for this effect is typically 2 - 6 ng/ml in the presence of 0.2 U/ml of rhEpo-Fc.
Handling
Centrifuge the vial prior to opening.

Product Categories/Family for EPOR active protein

Proteins and Enzymes; Human cell expressed recombinant proteinsCytokines; Growth Factors & Hormones; Human cell expressed recombinant proteinsProteins and Enzymes; Cytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A-Z) Proteins and Enzymes
Applications Tested/Suitable for EPOR active protein
SDS-PAGE

Testing Data of EPOR active protein

EPOR Active Protein – Cat# MBS844166

Other Notes

Small volumes of EPOR active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EPOR purchase

MBS844166 is an active protein targeting EPOR. Also known as Human CellExp EPOR / Erythropoietin Receptor, human recombinant. Host: HEK293 cells.

Additional Details

NCBI/Uniprot data below describe general gene information for EPOR. It may not necessarily be applicable to this product.

NCBI GI #
4503591
NCBI GeneID
2057
NCBI Accession #
NP_000112.1
UniProt Primary Accession #
P19235
UniProt Secondary Accession #
Q15443; Q2M205; B2RCG4
UniProt Related Accession #
P19235
GenBank Nucleotide #
NM_000121.3
NCBI Official Full Name
erythropoietin receptor
NCBI Official Synonym Full Names
erythropoietin receptor
NCBI Official Symbol
EPOR
NCBI Official Synonym Symbols
EPO-R
UniProt Protein Name
Erythropoietin receptor
Protein Family
Erythropoietin receptor
UniProt Gene Name
EPOR
UniProt Synonym Gene Names
EPO-R
UniProt Entry Name
EPOR_HUMAN
NCBI Protein Information
erythropoietin receptor

NCBI Summary for EPOR

This gene encodes the erythropoietin receptor which is a member of the cytokine receptor family. Upon erythropoietin binding, this receptor activates Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated erythropoietin receptor appears to have a role in erythroid cell survival. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Dysregulation of this gene may affect the growth of certain tumors. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]
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UniProt Comments for EPOR

EPOR: erythropoietin receptor: a member of the cytokine receptor family. Mediates erythropoietin-induced erythroblast proliferation, differentiation and survival. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. Forms homodimers on EPO stimulation. Tyrosine-phosphorylated EpoR may bind several SH2 domain-containing proteins including LYN, the adapter protein APS, SHP-1, SHP-2, JAK2, PI3 kinases, STAT5A/B, SOCS3, and CRKL. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Three alternatively-spliced isoforms have been described. Isoform EPOR-T, missing the cytoplasmic tail, acts as a dominant-negative receptor of EPOR-mediated signaling.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 19p13.3-p13.2

Cellular Component: integral to plasma membrane; extracellular region

Molecular Function: identical protein binding; protein binding; erythropoietin receptor activity

Biological Process: heart development; decidualization; brain development; signal transduction

Disease: Erythrocytosis, Familial, 1
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Research Articles on EPOR

1. transmembrane domain and the juxtamembrane region of the erythropoietin receptor in micelles

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with EPOR active protein

 Reference Product  PubMed Publications
 EPO active protein  >748 publications with EPOR and EPO
 JAK2 active protein  >199 publications with EPOR and JAK2
 KIT active protein  >51 publications with EPOR and KIT
 STAT5A active protein  >14 publications with EPOR and STAT5A
 GRB2 active protein  >10 publications with EPOR and GRB2
 STAT5B active protein  >9 publications with EPOR and STAT5B
 CRKL active protein  >6 publications with EPOR and CRKL
 LYN active protein  >6 publications with EPOR and LYN

Diseases associated with EPOR active protein

 Disease Name  Pubmed Publications
 Anemia Antibodies  >176 publications with EPOR and Anemia
 Polycythemia Antibodies  >80 publications with EPOR and Polycythemia
 Disease Models, Animal Antibodies  >68 publications with EPOR and Disease Models, Animal
 Kidney Diseases Antibodies  >44 publications with EPOR and Kidney Diseases
 Inflammation Antibodies  >35 publications with EPOR and Inflammation
 Neoplasms, Experimental Antibodies  >33 publications with EPOR and Neoplasms, Experimental
 Breast Neoplasms Antibodies  >32 publications with EPOR and Breast Neoplasms
 Necrosis Antibodies  >31 publications with EPOR and Necrosis
 Adenocarcinoma Antibodies  >29 publications with EPOR and Adenocarcinoma
 Heart Diseases Antibodies  >20 publications with EPOR and Heart Diseases

Organs/Tissues associated with EPOR active protein

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >362 publications with EPOR and Blood
 Bone Antibodies  >142 publications with EPOR and Bone
 Brain Antibodies  >118 publications with EPOR and Brain
 Kidney Antibodies  >97 publications with EPOR and Kidney
 Liver Antibodies  >77 publications with EPOR and Liver
 Heart Antibodies  >51 publications with EPOR and Heart
 Embryonic Tissue Antibodies  >33 publications with EPOR and Embryonic Tissue
 Lung Antibodies  >29 publications with EPOR and Lung
 Eye Antibodies  >26 publications with EPOR and Eye
 Skin Antibodies  >13 publications with EPOR and Skin
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