SCGF-Beta (SCF) Active Protein

Name: SCF Active Protein; SCGF-Beta; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS844233
Price: 355 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS844233

Background: SCGF alpha and beta are hematopoietic growth factor that exerts their activity at early stages of hematopoiesis. The SCGFs are non-glycosylated species-specific cytokines that can support growth of primitive hematopoietic cells, and in combination… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

SCGF-beta (SCF), Active Protein

Full Product Name

SCGF-beta, human recombinant

Product Gene Name

SCF active protein

Product Synonym Names

Stem Cell Growth Factor-beta

Sequence

MGHGARGAER EWEGGWGGAQ EEEREREALM LKHLQEALGL PAGRGDENPA GTVEGKEDWE MEEDQGEEEE EEATPTPSSG PSPSPTPEDI VTYILGRLAG LDAGLHQLHV RLHALDTRVV ELTQGLRQLR NAAGDTRDAV QALQEAQGRA EREHGRLEGC LKGLRLGHKC FLLSRDFEAQ PSASPHPLSP DQPNGGTLEN CVAQASDDGS WWDHDCQRRL YYVCEFPF

Host

E Coli

Form/Format

Sterile filtered through a 0.2 micron filter. Lyophilized from 0.25x PBS and 1mM DTT.
Appearance: Lyophilized powder

Purity/Purification

>=98%

Preparation and Storage

At -20 degree C
Shelf Life: 12 months

Molecular Weight

25.0 kDa

3D Structure

ModBase 3D Structure for P10721

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Solubility/Reconstitution Instructions

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20 degree C to -80 degree C.

Endotoxin Level

< 0.1 ng/mug of protein (<1EU/mug).

Activity (Specifications/test method)

Determined by the dose dependent stimulation of the proliferation of human TF-1 cells, previously grown in media containing GM-CSF. The expected ED50 for this effect is <=7.0 ng/ml.

Biological Activity

Determined by the dose dependent stimulation of the proliferation of human TF-1 cells, previously grown in media containing GM-CSF. The expected ED50 for this effect is <=7.0 ng/ml.

Handling

Centrifuge the vial prior to opening.

Product Categories/Family for SCF active protein

Cytokines; Growth Factors & Hormones; SCFsCytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A-Z) Proteins and Enzymes; Cytokines; Growth Factors & Hormones; SCFsProteins and Enzymes; Cytokines; Growth Factors & Hormones; Cytokines & Growth

Applications Tested/Suitable for SCF active protein

SDS-PAGE; HPLC

Other Notes

Small volumes of SCF active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SCF purchase

MBS844233 is an active protein targeting SCF. Also known as SCGF-beta, human recombinant. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for SCF. It may not necessarily be applicable to this product.

UniProt Primary Accession #

P10721

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.