Human Cellexp MMP-1 Active Protein – Cat# MBS844263

MMP-1 Human Cellexp Active Protein

Name: MMP-1 Active Protein; Human Cellexp; MyBioSource
Brand: MyBioSource
SKU: MBS844263
Price: 375 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS844263

Background: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes,… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

MMP-1, Active Protein

Full Product Name

Human CellExp MMP-1, human recombinant

Product Gene Name

MMP-1 active protein

Product Synonym Names

MMP1; CLG; CLGN

Host

HEK 293 cells

Form/Format

Lyophilized from 0.22 mum filtered solution in MES, NaCl, CaCl2 and Brij-35. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Appearance: Lyophilized powder

Purity/Purification

>=95%

Preparation and Storage

At -20 degree C
Shelf Life: 12 months

Molecular Weight

This protein is fused with 6×His tag at the N-terminus, has a calculated MW of 52.7 kDa. The predicted N-terminus is Phe 20. DTT-reduced Protein migrates as 52-55 kDa.

OMIM

120353

3D Structure

ModBase 3D Structure for P03956

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Solubility/Reconstitution Instructions

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.

Endotoxin Level

< 0.1 ng/mug of protein (<1EU/mug).

Activity (Specifications/test method)

Measured by its ability to cleave a fluorogenic peptide substrate Mca - KPLGL - Dpa - AR - NH2. The specific activity is > 200 pmol/min/ug, as measured under the described conditions.

Biological Activity

Measured by its ability to cleave a fluorogenic peptide substrate Mca - KPLGL - Dpa - AR - NH2. The specific activity is > 200 pmol/min/ug, as measured under the described conditions.

Handling

Centrifuge the vial prior to opening.

Product Categories/Family for MMP-1 active protein

Proteins and Enzymes; Human cell expressed recombinant proteinsProteins and Enzymes; Other Proteins & EnzymesProteins and Enzymes; Proteins and Enzymes (A-Z)

Applications Tested/Suitable for MMP-1 active protein

SDS-PAGE; HPLC

Other Notes

Small volumes of MMP-1 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MMP-1 purchase

MBS844263 is an active protein targeting MMP-1. Also known as Human CellExp MMP-1, human recombinant. Host: HEK 293 cells.

Additional Details

NCBI/Uniprot data below describe general gene information for MMP-1. It may not necessarily be applicable to this product.

NCBI GI #

4505215

NCBI GeneID

4312

NCBI Accession #

NP_002412.1

UniProt Primary Accession #

P03956

UniProt Secondary Accession #

P08156

UniProt Related Accession #

P03956

GenBank Nucleotide #

NM_002421.3

NCBI Official Full Name

interstitial collagenase isoform 1 preproprotein

NCBI Official Synonym Full Names

matrix metallopeptidase 1

NCBI Official Symbol

MMP1

NCBI Official Synonym Symbols

CLG; CLGN

UniProt Protein Name

Interstitial collagenase

UniProt Synonym Protein Names

Fibroblast collagenase; Matrix metalloproteinase-1; MMP-1

Protein Family

Interstitial collagenase

UniProt Gene Name

MMP1

UniProt Synonym Gene Names

CLG; MMP-1

UniProt Entry Name

MMP1_HUMAN

NCBI Protein Information

interstitial collagenase

NCBI Summary for MMP-1

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009]

UniProt Comments for MMP-1

MMP1: Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity. Belongs to the peptidase M10A family.

Protein type: Protease; Secreted; EC 3.4.24.7; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: proteinaceous extracellular matrix; extracellular region

Molecular Function: zinc ion binding; metalloendopeptidase activity; endopeptidase activity; calcium ion binding

Biological Process: positive regulation of protein oligomerization; collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; cellular protein metabolic process; viral reproduction; proteolysis; blood coagulation; leukocyte migration

Disease: Epidermolysis Bullosa Dystrophica, Autosomal Recessive; Pulmonary Disease, Chronic Obstructive

Research Articles on MMP-1

1. No evidence to support the association of MMP-1-1607 1G/2G and MMP-9-1562 C/T gene polymorphisms with spontaneous preterm birth in European Caucasian women.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with MMP-1 active protein

Reference Product	PubMed Publications
JUN active protein	>158 publications with MMP-1 and JUN
TIMP1 active protein	>150 publications with MMP-1 and TIMP1
TIMP2 active protein	>49 publications with MMP-1 and TIMP2
FOS active protein	>29 publications with MMP-1 and FOS
ETS1 active protein	>21 publications with MMP-1 and ETS1
ACAN active protein	>9 publications with MMP-1 and ACAN
BSG active protein	>6 publications with MMP-1 and BSG
SDC1 active protein	>1 publications with MMP-1 and SDC1
CTSG active protein	>1 publications with MMP-1 and CTSG
FOSL1 active protein	>1 publications with MMP-1 and FOSL1

Pathways associated with MMP-1 active protein

Products by Pathway	Pathway Diagram
Activation Of Matrix Metalloproteinases Pathway antibodies	Activation Of Matrix Metalloproteinases Pathway Diagram
Basigin Interactions Pathway antibodies	Basigin Interactions Pathway Diagram
Bladder Cancer Pathway antibodies	Bladder Cancer Pathway Diagram
Bladder Cancer Pathway antibodies	Bladder Cancer Pathway Diagram
Cell Surface Interactions At The Vascular Wall Pathway antibodies	Cell Surface Interactions At The Vascular Wall Pathway Diagram
Collagen Degradation Pathway antibodies	Collagen Degradation Pathway Diagram
Degradation Of The Extracellular Matrix Pathway antibodies	Degradation Of The Extracellular Matrix Pathway Diagram
Endothelins Pathway antibodies	Endothelins Pathway Diagram
Extracellular Matrix Organization Pathway antibodies	Extracellular Matrix Organization Pathway Diagram
Glucocorticoid Receptor Regulatory Network Pathway antibodies	Glucocorticoid Receptor Regulatory Network Pathway Diagram

Diseases associated with MMP-1 active protein

Disease Name	Pubmed Publications
Inflammation Antibodies	>145 publications with MMP-1 and Inflammation
Necrosis Antibodies	>91 publications with MMP-1 and Necrosis
Neoplasm Invasiveness Antibodies	>91 publications with MMP-1 and Neoplasm Invasiveness
Fibrosis Antibodies	>90 publications with MMP-1 and Fibrosis
Lung Diseases Antibodies	>85 publications with MMP-1 and Lung Diseases
Neoplasm Metastasis Antibodies	>74 publications with MMP-1 and Neoplasm Metastasis
Breast Neoplasms Antibodies	>63 publications with MMP-1 and Breast Neoplasms
Lung Neoplasms Antibodies	>49 publications with MMP-1 and Lung Neoplasms
Heart Diseases Antibodies	>41 publications with MMP-1 and Heart Diseases
Nervous System Diseases Antibodies	>35 publications with MMP-1 and Nervous System Diseases

Organs/Tissues associated with MMP-1 active protein

Organ/Tissue Name	Pubmed Publications
Skin Antibodies	>173 publications with MMP-1 and Skin
Blood Antibodies	>162 publications with MMP-1 and Blood
Lung Antibodies	>116 publications with MMP-1 and Lung
Vascular Antibodies	>106 publications with MMP-1 and Vascular
Bone Antibodies	>101 publications with MMP-1 and Bone
Connective Tissue Antibodies	>95 publications with MMP-1 and Connective Tissue
Muscle Antibodies	>60 publications with MMP-1 and Muscle
Liver Antibodies	>39 publications with MMP-1 and Liver
Embryonic Tissue Antibodies	>36 publications with MMP-1 and Embryonic Tissue
Brain Antibodies	>34 publications with MMP-1 and Brain