YbeY Recombinant Protein – Cat# MBS844281

Benzonase (YbeY) Recombinant Protein

Name: YbeY Recombinant Protein; Benzonase; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS844281
Price: 205 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS844281

Background: Golden Nuclease is a recombinant form of Serratia macescens extracellular endonuclease produced in Escherichia coli. Golden Nuclease is a homodimer with monomer molecular masses about 30 kDa. Two disulfide bonds found in the nuclease are crucial… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

Benzonase (YbeY), Recombinant Protein

Full Product Name

Benzonase, E. Coli Recombinant

Product Gene Name

YbeY recombinant protein

Product Synonym Names

Endoribonuclease

Host

E. coli

Form/Format

Liquid; 250 U/ul in 50% glycerol

Purity/Purification

> 98% by SDS-PAGE.

Preparation and Storage

Store at -20°C in aliquots.

Molecular Weight

~ 30 kDa

3D Structure

ModBase 3D Structure for P0A898

Working Conditions

- Optimal pH 8-8.5, functional pH 6-10.
- Optimal temperature 35°C - 42°C, functional 0°C to 42°C.
- Magnesium (1-2 mM) is required for activity. In the presence of 1 mM MgCl2, activity is reduced 30% by 1mM EDTA or completely nactivated by 0.1M EDTA. Activity is reduced 75% by 0.1 M CaCl2 or 1M NaCl.
- Under standard assay conditions, 1 mM iodoacetate had no effect on enzymatic activity. 1 mM mercaptoethanol and maleic acid educed tivity 5 to 10%. 10 mM p-Chloromercurybenzoate completely inactivates the enzyme. 0.64 M beta-mercaptoethanol in the presence of 2 M urea slightly decreases enzymatic activity. 4 to 7 M Urea increases enzymatic activity.

Unit definition

One unit is defined as the change of A260 = 1 (equivalent to 37 ug of DNA) when sonicated salmon sperm DNA is digested with the enzyme for 30 min at 37°C at pH 8.0.

Product Categories/Family for YbeY recombinant protein

Molecular Biology Tools; Molecular Biology Proteins/EnzymesProteins and Enzymes; Animal-Free Origin (AOF) Recombinant Proteins & EnzymesProteins and Enzymes; Proteins and Enzymes (A-Z)

Applications Tested/Suitable for YbeY recombinant protein

Removal of DNA/RNA from proteins; Purification of recombinant protein from inclusion bodies; Viscosity reduction of protein samples, etc.

Other Notes

Small volumes of YbeY recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for YbeY purchase

MBS844281 is a recombinant protein targeting YbeY. Also known as Benzonase, E. Coli Recombinant. Host: E. coli.

Additional Details

NCBI/Uniprot data below describe general gene information for YbeY. It may not necessarily be applicable to this product.

NCBI GI #

16128642

NCBI GeneID

946430

NCBI Accession #

NP_415192.1

UniProt Primary Accession #

P0A898

UniProt Secondary Accession #

P77385

GenBank Nucleotide #

NC_000913.3

NCBI Official Full Name

ssRNA-specific endoribonuclease; 16S rRNA 3' end maturation and quality control co-endoribonuclease working with RNase R; rRNA transcription antitermination factor

NCBI Official Symbol

ybeY

NCBI Official Synonym Symbols

ECK0651; JW0656

UniProt Protein Name

Endoribonuclease YbeY

Protein Family

Endoribonuclease

UniProt Gene Name

ybeY

UniProt Entry Name

YBEY_ECOLI

NCBI Protein Information

ssRNA-specific endoribonuclease; 16S rRNA 3' end maturation and quality control co-endoribonuclease working with RNase R; rRNA transcription antitermination factor

NCBI Summary for YbeY

AFMB Structural Genomics target Number 122 (http://afmb.cnrs-mrs.fr/article171.html). [More information is available at EcoGene: EG13655]. YbeY shows similarity to metal-dependent hydrolases. [More information is available at EcoCyc: G6362].

UniProt Comments for YbeY

Single strand-specific metallo-endoribonuclease involved in late-stage 70S ribosome quality control and in maturation of the 3' terminus of the 16S rRNA. Acts together with the RNase R to eliminate defective 70S ribosomes, but not properly matured 70S ribosomes or individual subunits, by a process mediated specifically by the 30S ribosomal subunit. Involved in the processing of 16S, 23S and 5S rRNAs, with a particularly strong effect on maturation at both the 5'-and 3'-ends of 16S rRNA as well as maturation of the 5'-end of 23S and 5S rRNAs.

Research Articles on YbeY

1. evidence which tie YbeY to the transcription antitermination process

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.