Human Cellexp SERPIND1 Active Protein – Cat# MBS844303

SERPIND1 Human Cellexp Active Protein

Name: SERPIND1 Active Protein; Human Cellexp; MyBioSource
Brand: MyBioSource
SKU: MBS844303
Price: 350 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS844303

Background: Serpin D1 is also known as Heparin cofactor 2 (HCF2), Protease inhibitor leuserpin-2 (HLS2), Heparin cofactor II (HC-II), which belongs to the serpin family. HCF2 is expressed predominantly in liver. Thrombin inhibitor activated by the… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

SERPIND1, Active Protein

Full Product Name

Human CellExp SERPIND1, human recombinant

Product Gene Name

SERPIND1 active protein

Product Synonym Names

SerpinD1; HCF2; HLS2; HC-II

Host

HEK293 cells

Form/Format

Lyophilized from 0.22 mum filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Appearance: Lyophilized

Purity/Purification

>=95%

Preparation and Storage

At -20 degree C
Shelf Life: 12 months

Molecular Weight

This protein is fused with polyhistidine tag at the C-terminus, has a calculated MW of 55.7 kDa. The predicted N-terminus is Gly 20. DTT-reduced Protein migrates as 65-70 kDa due to glycosylation.

OMIM

142360

3D Structure

ModBase 3D Structure for P05546

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Solubility/Reconstitution Instructions

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.

Endotoxin Level

<1 EU/mug by LAL method

Biological Activity

Measured by its ability to inhibit Recombinant Human Coagulation Factor II/Thrombin cleavage of a fluorogenic peptide substrate Boc-VPR-AMC. The IC50 value is <1.8 nM.

Results

Measured by its ability to inhibit Recombinant Human Coagulation Factor II/Thrombin cleavage of a fluorogenic peptide substrate Boc-VPR-AMC. The IC50 value is <1.8 nM.

Handling

Centrifuge the vial prior to opening.

Product Categories/Family for SERPIND1 active protein

Cytokines; Growth Factors & Hormones; Human cell expressed recombinant proteins Proteins and Enzymes; Human cell expressed recombinant proteins Cytokines; Growth Factors & Hormones; Human cell expressed recombinant proteinsCytokines; Growth Factors & Horm

Applications Tested/Suitable for SERPIND1 active protein

SDS-PAGE

Other Notes

Small volumes of SERPIND1 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SERPIND1 purchase

MBS844303 is an active protein targeting SERPIND1. Also known as Human CellExp SERPIND1, human recombinant. Host: HEK293 cells.

Additional Details

NCBI/Uniprot data below describe general gene information for SERPIND1. It may not necessarily be applicable to this product.

NCBI GI #

73858566

NCBI GeneID

3053

NCBI Accession #

NP_000176.2

UniProt Primary Accession #

P05546

UniProt Secondary Accession #

Q6IBZ5; B2RAI1; D3DX34

UniProt Related Accession #

P05546

GenBank Nucleotide #

NM_000185.3

NCBI Official Full Name

heparin cofactor 2

NCBI Official Synonym Full Names

serpin peptidase inhibitor, clade D (heparin cofactor), member 1

NCBI Official Symbol

SERPIND1

NCBI Official Synonym Symbols

HC2; LS2; HCF2; HCII; HLS2; THPH10; D22S673

UniProt Protein Name

Heparin cofactor 2

UniProt Synonym Protein Names

Heparin cofactor II; HC-II; Protease inhibitor leuserpin-2; HLS2; Serpin D1

Protein Family

Heparin cofactor

UniProt Gene Name

SERPIND1

UniProt Synonym Gene Names

HCF2; HC-II; HLS2

UniProt Entry Name

HEP2_HUMAN

NCBI Protein Information

heparin cofactor 2

NCBI Summary for SERPIND1

This gene belongs to the serpin gene superfamily. Serpins play roles in many processes including inflammation, blood clotting, and cancer metastasis. Members of this family have highly conserved secondary structures with a reactive center loop that interacts with the protease active site to inhibit protease activity. This gene encodes a plasma serine protease that functions as a thrombin and chymotrypsin inhibitor. The protein is activated by heparin, dermatan sulfate, and glycosaminoglycans. Allelic variations in this gene are associated with heparin cofactor II deficiency. [provided by RefSeq, Jul 2015]

UniProt Comments for SERPIND1

SERPIND1: Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT-III). Also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner. Defects in SERPIND1 are the cause of thrombophilia due to heparin cofactor 2 deficiency (THPH10). A hemostatic disorder characterized by a tendency to recurrent thrombosis. Belongs to the serpin family.

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: extracellular space; extracellular region

Molecular Function: heparin binding; serine-type endopeptidase inhibitor activity; endopeptidase inhibitor activity

Biological Process: chemotaxis; blood coagulation

Disease: Heparin Cofactor Ii Deficiency

Research Articles on SERPIND1

1. Molecular modelling of HCII 1-75 predicted those portions of the acidic extension that had been previously visualized in crystal structures, and suggested that an alpha-helix found between residues 26 and 36 stabilizes one found between residues 61-67.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with SERPIND1 active protein

Reference Product	PubMed Publications
PROC active protein	>1 publications with SERPIND1 and PROC

Pathways associated with SERPIND1 active protein

Products by Pathway	Pathway Diagram
Common Pathway Of Fibrin Clot Formation antibodies	Common Pathway Of Fibrin Clot Formation Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Formation Of Fibrin Clot (Clotting Cascade) Pathway antibodies	Formation Of Fibrin Clot (Clotting Cascade) Pathway Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram
Intrinsic Pathway Of Fibrin Clot Formation antibodies	Intrinsic Pathway Of Fibrin Clot Formation Diagram

Diseases associated with SERPIND1 active protein

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>5 publications with SERPIND1 and Cardiovascular Diseases
Hemolysis Antibodies	>1 publications with SERPIND1 and Hemolysis
Death Antibodies	>1 publications with SERPIND1 and Death
Lung Diseases Antibodies	>1 publications with SERPIND1 and Lung Diseases
Lung Neoplasms Antibodies	>1 publications with SERPIND1 and Lung Neoplasms
Liver Diseases Antibodies	>1 publications with SERPIND1 and Liver Diseases
Heart Diseases Antibodies	>1 publications with SERPIND1 and Heart Diseases
Hyperplasia Antibodies	>1 publications with SERPIND1 and Hyperplasia
Necrosis Antibodies	>1 publications with SERPIND1 and Necrosis
Heparin Cofactor II Deficiency Antibodies	>1 publications with SERPIND1 and Heparin Cofactor II Deficiency

Organs/Tissues associated with SERPIND1 active protein

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>5 publications with SERPIND1 and Liver
Vascular Antibodies	>3 publications with SERPIND1 and Vascular
Lung Antibodies	>2 publications with SERPIND1 and Lung
Kidney Antibodies	>1 publications with SERPIND1 and Kidney