Scd100/Semaphorin-4d (CD100) Active Protein

Name: CD100 Active Protein; Scd100/Semaphorin-4d; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS844307
Price: 320 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS844307

Background: The Semaphorins are a large family of phylogenetically conserved proteins that play a pivotal role in maintaining homeostasis in the immune system. Twenty members of this family have been identified and categorized into eight subclasses based on… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

sCD100/Semaphorin-4D, Active Protein

Full Product Name

sCD100 / Semaphorin-4D, Human Recombinant

Product Gene Name

CD100 active protein

Product Synonym Names

Semaphorin-4D; BB18; A8; GR3

Sequence

FAPIPRITWE HREVHLVQFH EPDIYNYSAL LLSEDKDTLY IGAREAVFAV NALNISEKQH EVYWKVSEDK KAKCAEKGKS KQTECLNYIR VLQPLSATSL YVCGTNAFQP ACDHLNLTSF KFLGKNEDGK GRCPFDPAHS YTSVMVDGEL YSGTSYNFLG SEPIISRNSS HSPLRTEYAI PWLNEPSFVF ADVIRKSPDS PDGEDDRVYF FFTEVSVEYE FVFRVLIPRI ARVCKGDQGG LRTLQKKWTS FLKARLICSR PDSGLVFNVL RDVFVLRSPG LKVPVFYALF TPQLNNVGLS AVCAYNLSTA EEVFSHGKYM QSTTVEQSHT KWVRYNGPVP KPRPGACIDS EARAANYTSS LNLPDKTLQF VKDHPLMDDS VTPIDNRPRL IKKDVNYTQI VVDRTQALDG TVYDVMFVST DRGALHKAIS LEHAVHIIEE TQLFQDFEPV QTLLLSSKKG NRFVYAGSNS GVVQAPLAFC GKHGTCEDCV LARDPYCAWS PPTATCVALH QTESPSRGLI QEMSGDASVC PDKSKGSYRQ HFFKHGGTAE LKCSQKSNLA RVFWKFQNGV LKAESPKYGL MGRKNLLIFN LSEGDSGVYQ CLSEERVKNK TVFQVVAKHV LEVKVVPKPV VAPTLSVVQT EGSRIATKVL VASTQGSSPP TPAVQATSSG AITLPPKPAP TGTSCEPKIV INTVPQLHSE KTMYLKSSDN R

Host

CHO Cells

Form/Format

Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.5.
Appearance: Lyophilized powder

Purity/Purification

>=97%

Preparation and Storage

At -20 degree C
Shelf Life: 12 months

Molecular Weight

78.9 kDa

OMIM

601866

3D Structure

ModBase 3D Structure for Q92854

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Solubility/Reconstitution Instructions

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20 degree C to -80 degree C.

Endotoxin Level

< 0.1 ng/mug of protein (<1EU/mug).

Activity (Specifications/test method)

Measured by its ability to inhibit chemokine (hMCP-3) induced human monocyte migration.

Biological Activity

Measured by its ability to inhibit chemokine (hMCP-3) induced human monocyte migration.

Handling

Centrifuge the vial prior to opening.

Product Categories/Family for CD100 active protein

Cytokines; Growth Factors & Hormones; CDs (NEW) Cytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A-Z) Proteins and Enzymes; Cytokines; Growth Factors & Hormones; CDs (NEW) Proteins and Enzymes; Cytokines; Growth Factors & Hormones; Cytoki

Applications Tested/Suitable for CD100 active protein

SDS-PAGE; HPLC

Other Notes

Small volumes of CD100 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD100 purchase

MBS844307 is an active protein targeting CD100. Also known as sCD100 / Semaphorin-4D, Human Recombinant. Host: CHO Cells.

Additional Details

NCBI/Uniprot data below describe general gene information for CD100. It may not necessarily be applicable to this product.

NCBI GI #

214010220

NCBI GeneID

10507

NCBI Accession #

NP_001135759.1

UniProt Primary Accession #

Q92854

UniProt Secondary Accession #

Q7Z5S4; Q8N8B0; B2RPM6

UniProt Related Accession #

Q92854

GenBank Nucleotide #

NM_001142287.1

NCBI Official Full Name

semaphorin-4D isoform 2

NCBI Official Synonym Full Names

sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D

NCBI Official Symbol

SEMA4D

NCBI Official Synonym Symbols

CD100; SEMAJ; coll-4; C9orf164; M-sema-G

UniProt Protein Name

Semaphorin-4D

UniProt Synonym Protein Names

A8; BB18; GR3; CD_antigen: CD100

UniProt Gene Name

SEMA4D

UniProt Synonym Gene Names

C9orf164; CD100; SEMAJ

UniProt Entry Name

SEM4D_HUMAN

NCBI Protein Information

semaphorin-4D

UniProt Comments for CD100

SEMA4D: a single-pass type I membrane protein that my play a functional role in the immune system, as well as in the nervous system. Induces B-cells to aggregate and improves their viability in vitro.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 9q22.2

Cellular Component: extracellular space; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; receptor activity; semaphorin receptor binding; receptor binding

Biological Process: negative regulation of peptidyl-tyrosine phosphorylation; axon guidance; regulation of dendrite morphogenesis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of cell adhesion; positive regulation of phosphoinositide 3-kinase cascade; regulation of cell shape; positive regulation of peptidyl-tyrosine phosphorylation; immune response; negative regulation of osteoblast differentiation; positive regulation of protein amino acid phosphorylation; cell adhesion; positive regulation of collateral sprouting; positive regulation of cell migration; negative regulation of apoptosis; regulation of cell projection organization and biogenesis

Research Articles on CD100

1. Sema4D contributes to enhanced invasion and tumor progression through increased motility of cervical cancer and VEGF-C/-D-mediated lymphangiogenesis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CD100 active protein

Reference Product	PubMed Publications
PLXNB1 active protein	>70 publications with CD100 and PLXNB1
CD72 active protein	>36 publications with CD100 and CD72
RHOA active protein	>21 publications with CD100 and RHOA
MET active protein	>18 publications with CD100 and MET
RND1 active protein	>4 publications with CD100 and RND1
RRAS active protein	>3 publications with CD100 and RRAS
ARHGEF12 active protein	>2 publications with CD100 and ARHGEF12
ARHGEF11 active protein	>2 publications with CD100 and ARHGEF11
ARHGAP35 active protein	>1 publications with CD100 and ARHGAP35
PLXNB2 active protein	>1 publications with CD100 and PLXNB2

Pathways associated with CD100 active protein

Products by Pathway	Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram
Other Semaphorin Interactions Pathway antibodies	Other Semaphorin Interactions Pathway Diagram
Sema4D In Semaphorin Signaling Pathway antibodies	Sema4D In Semaphorin Signaling Pathway Diagram
Sema4D Induced Cell Migration And Growth-cone Collapse Pathway antibodies	Sema4D Induced Cell Migration And Growth-cone Collapse Pathway Diagram
Sema4D Mediated Inhibition Of Cell Attachment And Migration Pathway antibodies	Sema4D Mediated Inhibition Of Cell Attachment And Migration Pathway Diagram
Semaphorin Interactions Pathway antibodies	Semaphorin Interactions Pathway Diagram

Diseases associated with CD100 active protein

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>26 publications with CD100 and Nervous System Diseases
Brain Diseases Antibodies	>15 publications with CD100 and Brain Diseases
Neovascularization, Pathologic Antibodies	>14 publications with CD100 and Neovascularization, Pathologic
Inflammation Antibodies	>13 publications with CD100 and Inflammation
Disease Models, Animal Antibodies	>11 publications with CD100 and Disease Models, Animal
Cardiovascular Diseases Antibodies	>9 publications with CD100 and Cardiovascular Diseases
Kidney Diseases Antibodies	>4 publications with CD100 and Kidney Diseases
Necrosis Antibodies	>4 publications with CD100 and Necrosis
Lung Diseases Antibodies	>3 publications with CD100 and Lung Diseases
Atherosclerosis Antibodies	>3 publications with CD100 and Atherosclerosis

Organs/Tissues associated with CD100 active protein

Organ/Tissue Name	Pubmed Publications
Nerve Antibodies	>98 publications with CD100 and Nerve
Blood Antibodies	>81 publications with CD100 and Blood
Brain Antibodies	>33 publications with CD100 and Brain
Embryonic Tissue Antibodies	>17 publications with CD100 and Embryonic Tissue
Skin Antibodies	>9 publications with CD100 and Skin
Spinal Cord Antibodies	>8 publications with CD100 and Spinal Cord
Kidney Antibodies	>7 publications with CD100 and Kidney
Lymph Node Antibodies	>7 publications with CD100 and Lymph Node
Lung Antibodies	>6 publications with CD100 and Lung
Liver Antibodies	>5 publications with CD100 and Liver