E.coli Recombinant G6PD Active Protein – Cat# MBS844386

G6PD E.coli Recombinant Active Protein

Name: G6PD Active Protein; E.coli Recombinant; MyBioSource
Brand: MyBioSource
SKU: MBS844386
Price: 445 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS844386

Background: Glucose-6-phosphate dehydrogenase is the rate-limiting enzyme of the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells by maintaining the level of NADPH. G6PD converts glucose-6-phosphate into… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

G6PD, Active Protein

Full Product Name

E.Coli Recombinant G6PD

Product Gene Name

G6PD active protein

Product Synonym Names

Glucose-6-phosphate 1-dehydrogenase; Zwf.; E.Coli Recombinant G6PD

Sequence

MAVTQTAQAC DLVIFGAKGD LARRKLLPSL YQLEKAGQLN PDTRIIGVGR ADWDKAAYTK VVREALETFM KETIDEGLWD TLSARLDFCN LDVNDTAAFS RLGAMLDQKN RITINYFAMP PSTFGAICKG LGEAKLNAKP ARVVMEKPLG TSLATSQEIN DQVGEYFEEC QVYRIDHYLG KETVLNLLAL RFANSLFVNN WDNRTIDHVE ITVAEEVGIE GRWGYFDKAG QMRDMIQNHL LQILCMIAMS PPSDLSADSI RDEKVKVLKS LRRIDRSNVR EKTVRGQYTA GFAQGKKVPG YLEEEGANKS SNTETFVAIR VDIDNWRWAG VPFYLRTGKR LPTKCSEVVV YFKTPELNLF KESWQDLPQN KLTIRLQPDE GVDIQVLNKV PGLDHKHNLQ ITKLDLSYSE TFNQTHLADA YERLLLETMR GIQALFVRRD EVEEAWKWVD SITEAWAMDN DAPKPYQAGT WGPVASVAMI TRDGRSWNEF E

Host

E Coli

Form/Format

1 mg/ml solution in 50 mM MES pH 6.0, 0.1 mM PMSF, 2 mM EDTA, 0.5 mM DTT and 10% glycerol.
Appearance: Liquid

Purity/Purification

>=90%

Concentration

1 mg/ml (lot specific)

Preparation and Storage

At -80 degree C
Shelf Life: 12 months

Molecular Weight

55.7 kDa (491 aa, 1-491 aa)

3D Structure

ModBase 3D Structure for P0AC53

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Biological Activity

Specific activity is 8-10 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NAD or NADP.

Results

8-10 units/ml

Unit Definition

One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25 degree C.

Handling

Centrifuge the vial prior to opening.

Product Categories/Family for G6PD active protein

Proteins and Enzymes; Proteins and Enzymes (A-Z)

Applications Tested/Suitable for G6PD active protein

SDS-PAGE

Other Notes

Small volumes of G6PD active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for G6PD purchase

MBS844386 is an active protein targeting G6PD. Also known as E.Coli Recombinant G6PD. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for G6PD. It may not necessarily be applicable to this product.

NCBI GI #

16129805

NCBI GeneID

946370

NCBI Accession #

NP_416366.1

UniProt Primary Accession #

P0AC53

UniProt Secondary Accession #

P22992; P78069; Q60134; Q60139

GenBank Nucleotide #

NC_000913.3

NCBI Official Full Name

glucose-6-phosphate 1-dehydrogenase

NCBI Official Symbol

zwf

NCBI Official Synonym Symbols

ECK1853; JW1841

UniProt Protein Name

Glucose-6-phosphate 1-dehydrogenase

Protein Family

Glucose-6-phosphate 1-dehydrogenase

UniProt Gene Name

zwf

UniProt Synonym Gene Names

G6PD; EDF

UniProt Entry Name

G6PD_ECOLI

NCBI Protein Information

glucose-6-phosphate 1-dehydrogenase

NCBI Summary for G6PD

Residues 199-203 of zwf encode a NNWDN peptide involved in ChpA(MazF) toxicity that is likely converted to NNWNN by AsnA; a zwf deletion prevents NNWNN activity (Kolodkin-Gal, 2007). [More information is available at EcoGene: EG11221]. A null mutation in the zwf gene encoding glucose 6-phosphate-1-dehydrogenase does not affect the growth rate significantly. [More information is available at EcoCyc: EG11221].

UniProt Comments for G6PD

Catalyzes the oxidation of glucose 6-phosphate to 6-phosphogluconolactone.

Research Articles on G6PD

1. G6PDH and FPR could act concertedly to deliver reducing equivalents from carbohydrates, via NADP(+), to the FPR acceptors ferredoxin and/or flavodoxin.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with G6PD active protein

Reference Product	PubMed Publications
gnd active protein	>34 publications with G6PD and gnd
pgi active protein	>30 publications with G6PD and pgi
soxS active protein	>21 publications with G6PD and soxS
pykF active protein	>5 publications with G6PD and pykF
tktA active protein	>3 publications with G6PD and tktA
eno active protein	>3 publications with G6PD and eno
tpiA active protein	>3 publications with G6PD and tpiA
pykA active protein	>1 publications with G6PD and pykA

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