CYR61 Active Protein

Name: CYR61 Active Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS844396
Price: 295 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS844396

Background: CYR61 is a member of the CCN family of secreted cysteine rich regulatory proteins. CYR61 induces angiogenesis by stimulating the proliferation, migration, and adhesion of endothelial cells. Cell migration and adhesion are mediated through binding… More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

CYR61, Active Protein

Full Product Name

CYR61, Human Recombinant

Product Gene Name

CYR61 active protein

Product Synonym Names

CCN1; G1G1 protein

Sequence

MTCPAACHCP LEAPKCAPGV GLVRDGCGCC KVCAKQLNED CSKTQPCDHT KGLECNFGAS STALKGICRA QSEGRPCEYN SRIYQNGESF QPNCKHQCTC IDGAVGCIPL CPQELSLPNL GCPNPRLVKV TGQCCEEWVC DEDSIKDPME DQDGLLGKEL GFDASEVELT RNNELIAVGK GSSLKRLPVF GMEPRILYNP LQGQKCIVQT TSWSQCSKTC GTGISTRVTN DNPECRLVKE TRICEVRPCG QPVYSSLKKG KKCSKTKKSP EPVRFTYAGC LSVKKYRPKY CGSCVDGRCC TPQLTRTVKM RFRCEDGETF SKNVMMIQSC KCNYNCPHAN EAAFPFYRLF NDIHKFRD

Host

E Coli

Form/Format

Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Citrate, pH 3.0 and 50 mM NaCl.
Appearance: Lyophilized powder

Purity/Purification

>=95%

Preparation and Storage

At -20 degree C
Shelf Life: 12 months

Molecular Weight

39.4 kDa

OMIM

602369

3D Structure

ModBase 3D Structure for O00622

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Solubility/Reconstitution Instructions

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not raise to neutral pH. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20 degree C to -80 degree C.

Endotoxin Level

< 0.1 ng/mug of protein (<1EU/mug).

Activity (Specifications/test method)

Determined by the dose-dependent stimulation of the proliferation of murine 3T3 cells. The expected ED50 for this effect is 2.0-3.0 mug/ml.

Biological Activity

Determined by the dose-dependent stimulation of the proliferation of murine 3T3 cells. The expected ED50 for this effect is 2.0-3.0 mug/ml.

Handling

Centrifuge the vial prior to opening.

Product Categories/Family for CYR61 active protein

Cytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A-Z) Proteins and Enzymes; Cytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A-Z) Proteins and Enzymes; Proteins and Enzymes (A-Z) Proteins and Enzymes; Animal-Free Origin

Applications Tested/Suitable for CYR61 active protein

SDS-PAGE; HPLC

Other Notes

Small volumes of CYR61 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CYR61 purchase

MBS844396 is an active protein targeting CYR61. Also known as CYR61, Human Recombinant. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for CYR61. It may not necessarily be applicable to this product.

NCBI GI #

31542331

NCBI GeneID

3491

NCBI Accession #

NP_001545.2

UniProt Primary Accession #

O00622

UniProt Secondary Accession #

O14934; O43775; Q9BZL7

UniProt Related Accession #

O00622

GenBank Nucleotide #

NM_001554.4

NCBI Official Full Name

protein CYR61

NCBI Official Synonym Full Names

cysteine-rich, angiogenic inducer, 61

NCBI Official Symbol

CYR61

NCBI Official Synonym Symbols

CCN1; GIG1; IGFBP10

UniProt Protein Name

Protein CYR61

UniProt Synonym Protein Names

CCN family member 1; Cysteine-rich angiogenic inducer 61; Insulin-like growth factor-binding protein 10; IBP-10; IGF-binding protein 10; IGFBP-10; Protein GIG1

Protein Family

Protein

UniProt Gene Name

CYR61

UniProt Synonym Gene Names

CCN1; GIG1; IGFBP10; IBP-10; IGF-binding protein 10; IGFBP-10

UniProt Entry Name

CYR61_HUMAN

NCBI Protein Information

protein CYR61

NCBI Summary for CYR61

The secreted protein encoded by this gene is growth factor-inducible and promotes the adhesion of endothelial cells. The encoded protein interacts with several integrins and with heparan sulfate proteoglycan. This protein also plays a role in cell proliferation, differentiation, angiogenesis, apoptosis, and extracellular matrix formation. [provided by RefSeq, Sep 2011]

UniProt Comments for CYR61

CYR61: Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up- regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3. Belongs to the CCN family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1p22.3

Cellular Component: extracellular matrix; proteinaceous extracellular matrix

Molecular Function: heparin binding; integrin binding; insulin-like growth factor binding; extracellular matrix binding

Biological Process: extracellular matrix organization and biogenesis; anatomical structure morphogenesis; positive regulation of caspase activity; chemotaxis; intussusceptive angiogenesis; osteoblast differentiation; cell proliferation; positive regulation of osteoblast differentiation; cell-cell signaling; positive regulation of protein kinase activity; positive regulation of osteoblast proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; regulation of cell growth; cell adhesion; positive regulation of BMP signaling pathway; negative regulation of apoptosis; positive regulation of cell migration

Research Articles on CYR61

1. Findings reveal that CCN1 plays a novel role in pathogen clearance; increasing macrophage infiltration and activation resulting in increased virus clearance in tumors.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CYR61 active protein

Reference Product	PubMed Publications
JUN active protein	>92 publications with CYR61 and JUN
FOS active protein	>20 publications with CYR61 and FOS
CTNNB1 active protein	>14 publications with CYR61 and CTNNB1
RHOA active protein	>10 publications with CYR61 and RHOA
SRF active protein	>5 publications with CYR61 and SRF
MKL1 active protein	>5 publications with CYR61 and MKL1
ITGAV active protein	>1 publications with CYR61 and ITGAV

Pathways associated with CYR61 active protein

Products by Pathway	Pathway Diagram
Hypertrophy Model Pathway antibodies	Hypertrophy Model Pathway Diagram
Oncostatin M Signaling Pathway antibodies	Oncostatin M Signaling Pathway Diagram
Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway antibodies	Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway Diagram
RhoA Signaling Pathway antibodies	RhoA Signaling Pathway Diagram
Amb2 Integrin Signaling Pathway antibodies	Amb2 Integrin Signaling Pathway Diagram

Diseases associated with CYR61 active protein

Disease Name	Pubmed Publications
Carcinoma Antibodies	>88 publications with CYR61 and Carcinoma
Inflammation Antibodies	>56 publications with CYR61 and Inflammation
Disease Models, Animal Antibodies	>47 publications with CYR61 and Disease Models, Animal
Neoplasm Metastasis Antibodies	>45 publications with CYR61 and Neoplasm Metastasis
Breast Neoplasms Antibodies	>42 publications with CYR61 and Breast Neoplasms
Fibrosis Antibodies	>39 publications with CYR61 and Fibrosis
Nervous System Diseases Antibodies	>31 publications with CYR61 and Nervous System Diseases
Necrosis Antibodies	>26 publications with CYR61 and Necrosis
Brain Diseases Antibodies	>23 publications with CYR61 and Brain Diseases
Neoplasms, Experimental Antibodies	>23 publications with CYR61 and Neoplasms, Experimental

Organs/Tissues associated with CYR61 active protein

Organ/Tissue Name	Pubmed Publications
Connective Tissue Antibodies	>185 publications with CYR61 and Connective Tissue
Vascular Antibodies	>131 publications with CYR61 and Vascular
Bone Antibodies	>82 publications with CYR61 and Bone
Muscle Antibodies	>77 publications with CYR61 and Muscle
Embryonic Tissue Antibodies	>56 publications with CYR61 and Embryonic Tissue
Lung Antibodies	>51 publications with CYR61 and Lung
Brain Antibodies	>39 publications with CYR61 and Brain
Prostate Antibodies	>33 publications with CYR61 and Prostate
Skin Antibodies	>32 publications with CYR61 and Skin
Liver Antibodies	>28 publications with CYR61 and Liver