Figure. The binding activity of MK with LRP1.
Midkine (MK) also known as neurite growth-promoting factor 2 (NEGF2) is a protein that in humans is encoded by the MDK gene. MK is a basic heparin-binding growth factor of low molecular weight, and forms a family with pleiotrophin. It is pleiotropic, capable of exerting activities such as cell proliferation, cell migration, angiogenesis and fibrinolysis. Besides, Low Density Lipoprotein Receptor Related Protein 1 (LRP1) has been identified as an interactor of MK, thus a binding ELISA assay was conducted to detect the interaction of recombinant human MK and recombinant human LRP1. Briefly, MK were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to LRP1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-MK pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of MK and LRP1 was shown in Figure 1, and this effect was in a dose dependent manner.

Midkine (MK) Active Protein

Name: MK Active Protein; Midkine; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS2105106
Price: 310 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2105106

MBS2105106 is an active protein targeting MK. Also known as Active Midkine (MK). More details

$310 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Midkine (MK), Active Protein

Full Product Name

Active Midkine (MK)

Product Gene Name

MK active protein

Product Synonym Names

MDK; ARAP; NEGF2; Neurite Growth-Promoting Factor 2; Amphiregulin-associated protein; Midgestation and kidney protein; Neurite outgrowth-promoting factor 2

Form/Format

Freeze-Dried Powder; 20mM Tris, 150mM NaCl, pH8.0, Containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.

Purity/Purification

> 95%

Preparation and Storage

Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Avoid repeated freeze/thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

OMIM

162096

3D Structure

ModBase 3D Structure for P21741

Supply Chain Verification

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Species

Human

Tag

No-Tag

Isoelectric Point

9.4

Subcellular Location

Secreted

Reconstitution

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

ISO Certification

Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.

Applications Tested/Suitable for MK active protein

Cell Culture, Activity Assays

Other Notes

Small volumes of MK active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MK. It may not necessarily be applicable to this product.

NCBI GI #

396080281

NCBI GeneID

4192

NCBI Accession #

NP_001257480.1

UniProt Primary Accession #

P21741

UniProt Related Accession #

P21741

GenBank Nucleotide #

NM_001270551.1

NCBI Official Full Name

midkine isoform a

NCBI Official Synonym Full Names

midkine

NCBI Official Symbol

MDK

NCBI Official Synonym Symbols

MK; ARAP; NEGF2

UniProt Protein Name

Midkine

UniProt Synonym Protein Names

Amphiregulin-associated protein; ARAP; Midgestation and kidney protein; Neurite outgrowth-promoting factor 2; Neurite outgrowth-promoting protein

Protein Family

Midkine

UniProt Gene Name

MDK

UniProt Synonym Gene Names

MK1; NEGF2; MK; ARAP

UniProt Entry Name

MK_HUMAN

NCBI Protein Information

midkine

NCBI Summary for MK

This gene encodes a member of a small family of secreted growth factors that binds heparin and responds to retinoic acid. The encoded protein promotes cell growth, migration, and angiogenesis, in particular during tumorigenesis. This gene has been targeted as a therapeutic for a variety of different disorders. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2012]

UniProt Comments for MK

MDK: Developmentally regulated, secreted growth factor homologous to pleiotrophin (PTN), which has heparin binding activity. Binds anaplastic lymphoma kinase (ALK) which induces ALK activation and subsequent phosphorylation of the insulin receptor substrate (IRS1), followed by the activation of mitogen-activated protein kinase (MAPK) and PI3-kinase, and the induction of cell proliferation. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development. Belongs to the pleiotrophin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: cell projection; cytoplasm; extracellular region

Molecular Function: heparin binding; growth factor activity

Biological Process: response to drug; nervous system development; cell migration; Notch signaling pathway; dentate gyrus development; behavioral fear response; cerebellar granular layer development; short-term memory; adrenal gland development; positive regulation of transcription, DNA-dependent; response to glucocorticoid stimulus; signal transduction; positive regulation of cell division; response to wounding; cerebral cortex development; negative regulation of neuron apoptosis; cell differentiation; defecation; regulation of behavior

Research Articles on MK

1. Studied levels of mucin 16 (CA125), adenosine deaminase and midkine as tumor markers in nonsmall cell lung cancer-associated malignant pleural effusion.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with MK active protein

Reference Product	PubMed Publications
ACE active protein	>2 publications with MK and ACE

Pathways associated with MK active protein

Products by Pathway	Pathway Diagram
Glypican 2 Network Pathway antibodies	Glypican 2 Network Pathway Diagram
NOTCH2 Activation And Transmission Of Signal To The Nucleus Pathway antibodies	NOTCH2 Activation And Transmission Of Signal To The Nucleus Pathway Diagram
Signal Transduction Pathway antibodies	Signal Transduction Pathway Diagram
Signaling By NOTCH Pathway antibodies	Signaling By NOTCH Pathway Diagram
Signaling By NOTCH2 Pathway antibodies	Signaling By NOTCH2 Pathway Diagram
Syndecan-4-mediated Signaling Events Pathway antibodies	Syndecan-4-mediated Signaling Events Pathway Diagram

Diseases associated with MK active protein

Disease Name	Pubmed Publications
Neoplasms Antibodies	>62 publications with MK and Neoplasms
Kidney Diseases Antibodies	>23 publications with MK and Kidney Diseases
Nervous System Diseases Antibodies	>17 publications with MK and Nervous System Diseases
Adenocarcinoma Antibodies	>13 publications with MK and Adenocarcinoma
Brain Diseases Antibodies	>11 publications with MK and Brain Diseases
Congenital Abnormalities Antibodies	>11 publications with MK and Congenital Abnormalities
Inflammation Antibodies	>9 publications with MK and Inflammation
Skin Diseases Antibodies	>9 publications with MK and Skin Diseases
Pain Antibodies	>6 publications with MK and Pain
Lung Neoplasms Antibodies	>6 publications with MK and Lung Neoplasms

Organs/Tissues associated with MK active protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>39 publications with MK and Blood
Kidney Antibodies	>25 publications with MK and Kidney
Brain Antibodies	>19 publications with MK and Brain
Lung Antibodies	>13 publications with MK and Lung
Bone Antibodies	>13 publications with MK and Bone
Embryonic Tissue Antibodies	>10 publications with MK and Embryonic Tissue
Muscle Antibodies	>9 publications with MK and Muscle
Liver Antibodies	>8 publications with MK and Liver
Skin Antibodies	>6 publications with MK and Skin
Lymph Node Antibodies	>5 publications with MK and Lymph Node