FCGR3A/Cd16a (FCGR3A) Active Protein

Name: FCGR3A Active Protein; FCGR3A/Cd16a; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS844458
Price: 345 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS844458

Background: CD16 is a low affinity Fc receptor, and has been identified as Fc receptors FcgammaRIIIa (CD16a) and Fcgamma IIIb (CD16b). These receptors bind to the Fc portion of IgG antibodies. CD16 encoded by two different highly homologous genes in a cell… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

FCGR3A/CD16a, Active Protein

Full Product Name

Human CellExp FCGR3A / CD16a, human recombinant

Product Gene Name

FCGR3A active protein

Product Synonym Names

FCGR3A; CD16A; FCG3; FCGR3; IGFR3

Host

HEK293 cells

Form/Format

Lyophilized from 0.22 mum filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Appearance: Lyophilized

Purity/Purification

>=98%

Preparation and Storage

At -20 degree C
Shelf Life: 12 months

Molecular Weight

This protein is fused with 6×his tag at the C-terminus, and has a calculated MW of 22.7 kDa. The predicted N-terminus is Gly 17. DTT-reduced Protein migrates as 36-45 kDa in SDS-PAGE due to glycosylation.

OMIM

146740

3D Structure

ModBase 3D Structure for P08637

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Solubility/Reconstitution Instructions

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.

Endotoxin Level

<1 EU/mug by LAL method

Biological Activity

Measured by its ability to bind human IgG with an estimated KD < 45 nM.

Results

Measured by its ability to bind human IgG with an estimated KD < 45 nM.

Handling

Centrifuge the vial prior to opening.

Product Categories/Family for FCGR3A active protein

Cytokines; Growth Factors & Hormones; Human cell expressed recombinant proteins Proteins and Enzymes; Human cell expressed recombinant proteinsProteins and Enzymes; Cytokines; Growth Factors & Hormones; CDsProteins and Enzymes; Cytokines; Growth Factors &

Applications Tested/Suitable for FCGR3A active protein

SDS-PAGE

Other Notes

Small volumes of FCGR3A active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FCGR3A purchase

MBS844458 is an active protein targeting FCGR3A. Also known as Human CellExp FCGR3A / CD16a, human recombinant. Host: HEK293 cells.

Additional Details

NCBI/Uniprot data below describe general gene information for FCGR3A. It may not necessarily be applicable to this product.

NCBI GI #

50726979

NCBI GeneID

2214

NCBI Accession #

NP_000560.5

UniProt Primary Accession #

P08637

UniProt Secondary Accession #

Q53FJ0; Q53FL6; Q5EBR4; Q65ZM6; Q6PIJ0; A2N6W9

UniProt Related Accession #

P08637

GenBank Nucleotide #

NM_000569.6

NCBI Official Full Name

low affinity immunoglobulin gamma Fc region receptor III-A isoform a

NCBI Official Synonym Full Names

Fc fragment of IgG, low affinity IIIa, receptor (CD16a)

NCBI Official Symbol

FCGR3A

NCBI Official Synonym Symbols

CD16; FCG3; CD16A; FCGR3; IGFR3; IMD20; FCR-10; FCRIII; FCGRIII; FCRIIIA

UniProt Protein Name

Low affinity immunoglobulin gamma Fc region receptor III-A

UniProt Synonym Protein Names

CD16a antigen; Fc-gamma RIII-alpha; Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa; FcR-10; IgG Fc receptor III-2; CD_antigen: CD16a

Protein Family

Low affinity immunoglobulin gamma Fc region receptor

UniProt Gene Name

FCGR3A

UniProt Synonym Gene Names

CD16A; FCG3; FCGR3; IGFR3; Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa

UniProt Entry Name

FCG3A_HUMAN

NCBI Protein Information

low affinity immunoglobulin gamma Fc region receptor III-A

NCBI Summary for FCGR3A

This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other other antibody-dependent responses. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

UniProt Comments for FCGR3A

FCGR3A: Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23

Cellular Component: plasma membrane; integral to membrane; external side of plasma membrane

Molecular Function: IgG binding

Biological Process: regulation of immune response; innate immune response; immune response

Disease: Immunodeficiency 20

Research Articles on FCGR3A

1. No differences in polymorphisms were found between the FCGR polymorphisms HH and the others and VV versus the others in KRAS-WT mCRC patients refractory to irinotecan, oxaliplatin and 5-fluorouracil treated with cetuximab.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with FCGR3A active protein

Reference Product	PubMed Publications
FCGR2A active protein	>73 publications with FCGR3A and FCGR2A
FCGR2B active protein	>27 publications with FCGR3A and FCGR2B
PIK3CA active protein	>2 publications with FCGR3A and PIK3CA
SYK active protein	>2 publications with FCGR3A and SYK

Pathways associated with FCGR3A active protein

Products by Pathway	Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
FCGR Activation Pathway antibodies	FCGR Activation Pathway Diagram
Fc Gamma R-mediated Phagocytosis Pathway antibodies	Fc Gamma R-mediated Phagocytosis Pathway Diagram
Fc Gamma R-mediated Phagocytosis Pathway antibodies	Fc Gamma R-mediated Phagocytosis Pathway Diagram
Fcgamma Receptor (FCGR) Dependent Phagocytosis Pathway antibodies	Fcgamma Receptor (FCGR) Dependent Phagocytosis Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway antibodies	Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway Diagram
Innate Immune System Pathway antibodies	Innate Immune System Pathway Diagram
Leishmaniasis Pathway antibodies	Leishmaniasis Pathway Diagram
Leishmaniasis Pathway antibodies	Leishmaniasis Pathway Diagram

Diseases associated with FCGR3A active protein

Disease Name	Pubmed Publications
Necrosis Antibodies	>28 publications with FCGR3A and Necrosis
Lupus Erythematosus, Systemic Antibodies	>26 publications with FCGR3A and Lupus Erythematosus, Systemic
Cardiovascular Diseases Antibodies	>23 publications with FCGR3A and Cardiovascular Diseases
Inflammation Antibodies	>19 publications with FCGR3A and Inflammation
Leukemia Antibodies	>14 publications with FCGR3A and Leukemia
Breast Neoplasms Antibodies	>14 publications with FCGR3A and Breast Neoplasms
Kidney Diseases Antibodies	>12 publications with FCGR3A and Kidney Diseases
Neoplasms, Experimental Antibodies	>12 publications with FCGR3A and Neoplasms, Experimental
Hemorrhage Antibodies	>8 publications with FCGR3A and Hemorrhage
Leukemia, Lymphoid Antibodies	>7 publications with FCGR3A and Leukemia, Lymphoid

Organs/Tissues associated with FCGR3A active protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>167 publications with FCGR3A and Blood
Kidney Antibodies	>10 publications with FCGR3A and Kidney
Ovary Antibodies	>6 publications with FCGR3A and Ovary
Lung Antibodies	>5 publications with FCGR3A and Lung
Liver Antibodies	>4 publications with FCGR3A and Liver
Brain Antibodies	>3 publications with FCGR3A and Brain
Intestine Antibodies	>1 publications with FCGR3A and Intestine
Thyroid Antibodies	>1 publications with FCGR3A and Thyroid
Spleen Antibodies	>1 publications with FCGR3A and Spleen
Stomach Antibodies	>1 publications with FCGR3A and Stomach