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IFN-beta 1b Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS844466

Background: Recombinant Human IFN-beta 1b produced from E.Coli is a single, non-glycosylated human Interferon-beta 1b polypeptide chain containing 165 amino acids and having a molecular mass of 18.51 kDa. The Interferon-beta 1b gene was cloned from human… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
IFN-beta 1b, Active Protein
Full Product Name
IFN-beta 1b, human recombinant
Product Synonym Names
Leukocyte interferon; B cell interferon; Type I interferon; IFNB1; IFB; IFF; IFNB; IFN-b 1b; MGC96956
Host
E Coli
Form/Format
Lyophilized from 1 mg/ml solution containing 50 mg human albumin and 50 mg dextrose.
Appearance: Lyophilized protein
Purity/Purification
>=98%
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Molecular Weight
18.5 kDa
OMIM
607402
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Solubility/Reconstitution Instructions
Reconstitute in H?O to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4 degree C for 1 week or -20 degree C for future use.
Endotoxin Level
<0.1 ng/mug
Biological Activity
MyBioSource's Interferon beta 1b is fully biologically active when compared to standard. The specific activity as determined in a viral resistance assay (human "Wish" cell line and VSV virus or the monkey VERO cell line with EMCV virus) was found to be 32 x 10 IU/ mg.
Handling
Centrifuge the vial prior to opening.
Applications Tested/Suitable for IFN-beta 1b active protein
SDS-PAGE; HPLC

Other Notes

Small volumes of IFN-beta 1b active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IFN-beta 1b purchase

MBS844466 is an active protein targeting IFN-beta 1b. Also known as IFN-beta 1b, human recombinant. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for IFN-beta 1b. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
interferon lambda-3
NCBI Official Synonym Full Names
interferon, lambda 3
NCBI Official Symbol
IFNL3
NCBI Official Synonym Symbols
IL28B; IL28C; IL-28B
UniProt Protein Name
Interferon lambda-3
UniProt Synonym Protein Names
Cytokine Zcyto22; Interleukin-28B; IL-28B; Interleukin-28C; IL-28C
UniProt Gene Name
IFNL3
UniProt Synonym Gene Names
IL28B; IL28C; ZCYTO22; IFN-lambda-3; IL-28B; IL-28C
UniProt Entry Name
IFNL3_HUMAN
NCBI Protein Information
interferon lambda-3

NCBI Summary for IFN-beta 1b

This gene encodes a cytokine distantly related to type I interferons and the IL-10 family. This gene, interleukin 28A (IL28A), and interleukin 29 (IL29) are three closely related cytokine genes that form a cytokine gene cluster on a chromosomal region mapped to 19q13. Expression of the cytokines encoded by the three genes can be induced by viral infection. All three cytokines have been shown to interact with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha (IL28RA). [provided by RefSeq, Jul 2008]
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UniProt Comments for IFN-beta 1b

IL-28B: Cytokine with immunomodulatory activity. Up-regulates MHC class I antigen expression. Displays potent antiviral activity. Also displays antitumor activity. Ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway. Belongs to the IL-28/IL-29 family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19q13.13

Cellular Component: extracellular space

Molecular Function: cytokine activity

Biological Process: positive regulation of immune response; negative regulation of viral genome replication; JAK-STAT cascade; defense response to virus

Disease: Hepatitis C Virus, Susceptibility To
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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