PPM1F Active Protein

Name: PPM1F Active Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS844493
Price: 460 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS844493

Background: PPM1F is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

PPM1F, Active Protein

Full Product Name

PPM1F, human recombinant

Product Gene Name

PPM1F active protein

Product Synonym Names

Protein phosphatase 1F; CAMKP; CaMKPase; FEM-2; hFEM-2; POPX2

Sequence

MGSSHHHHHH SSGLVPRGSH MGSMSSGAPQ KSSPMASGAE ETPGFLDTLL QDFPALLNPE DPLPWKAPGT VLSQEEVEGE LAELAMGFLG SRKAPPPLAA ALAHEAVSQL LQTDLSEFRK LPREEEEEEE DDDEEEKAPV TLLDAQSLAQ SFFNRLWEVA GQWQKQVPLA ARASQRQWLV SIHAIRNTRR KMEDRHVSLP SFNQLFGLSD PVNRAYFAVF DGHGGVDAAR YAAVHVHTNA ARQPELPTDP EGALREAFRR TDQMFLRKAK RERLQSGTTG VCALIAGATL HVAWLGDSQV ILVQQGQVVK LMEPHRPERQ DEKARIEALG GFVSHMDCWR VNGTLAVSRA IGDVFQKPYV

Host

E Coli

Form/Format

1 mg/ml solution 20 mM Tris-HCl buffer (pH 8.0), 20% glycerol, 150 mM NaCl and 1 mM DTT.
Appearance: Liquid

Purity/Purification

>=90%

Concentration

1 mg/ml (lot specific)

Preparation and Storage

At -20 degree C
Shelf Life: 12 months

Molecular Weight

52 kDa (477 aa, 1-454 aa + His Tag)

3D Structure

ModBase 3D Structure for P49593

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Biological Activity

Specific activity is > 1200 unit/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37 degree C, pH 7.5 using 10 mM of substrate.

Results

Specific activity is > 1200 unit/mg

Handling

Centrifuge the vial prior to opening.

Product Categories/Family for PPM1F active protein

Proteins and Enzymes; Proteins and Enzymes (A-Z) Cell Damage & Oxidative Stress; Enzymes

Applications Tested/Suitable for PPM1F active protein

SDS-PAGE

Other Notes

Small volumes of PPM1F active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PPM1F purchase

MBS844493 is an active protein targeting PPM1F. Also known as PPM1F, human recombinant. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for PPM1F. It may not necessarily be applicable to this product.

NCBI GI #

7661862

NCBI GeneID

9647

NCBI Accession #

NP_055449.1

UniProt Primary Accession #

P49593

UniProt Secondary Accession #

Q96PM2; A8K6G3; B7Z2C3

UniProt Related Accession #

P49593

GenBank Nucleotide #

NM_014634.3

NCBI Official Full Name

protein phosphatase 1F

NCBI Official Synonym Full Names

protein phosphatase, Mg2+/Mn2+ dependent, 1F

NCBI Official Symbol

PPM1F

NCBI Official Synonym Symbols

CAMKP; FEM-2; POPX2; hFEM-2; CaMKPase

UniProt Protein Name

Protein phosphatase 1F

UniProt Synonym Protein Names

Ca(2+)/calmodulin-dependent protein kinase phosphatase; CaM-kinase phosphatase; CaMKPase; Partner of PIX 2; Protein fem-2 homolog; hFem-2

Protein Family

Protein phosphatase

UniProt Gene Name

PPM1F

UniProt Synonym Gene Names

KIAA0015; POPX2; CaM-kinase phosphatase; CaMKPase; hFem-2

UniProt Entry Name

PPM1F_HUMAN

NCBI Protein Information

protein phosphatase 1F

NCBI Summary for PPM1F

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined. [provided by RefSeq, Jul 2008]

UniProt Comments for PPM1F

PPM1F: a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members can be negative regulators of cell stress response pathways. Interact with PAK-interacting exchange factor beta (ARHGEF7), blocking the effects PAK1, a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CaMK2-gamma) is one of the substrates of this phosphatase. Promotes apoptosis.

Protein type: Motility/polarity/chemotaxis; EC 3.1.3.16; Protein phosphatase, dual-specificity

Chromosomal Location of Human Ortholog: 22q11.22

Cellular Component: protein complex; perinuclear region of cytoplasm; cytosol

Molecular Function: protein binding; calmodulin-dependent protein phosphatase activity; metal ion binding; phosphoric monoester hydrolase activity; protein serine/threonine phosphatase activity

Biological Process: histone dephosphorylation; positive regulation of focal adhesion formation; positive regulation of chemotaxis; positive regulation of stress fiber formation; negative regulation of peptidyl-serine phosphorylation; negative regulation of protein kinase activity; positive regulation of caspase activity; negative regulation of transcription, DNA-dependent; positive regulation of growth

Research Articles on PPM1F

1. POPX2 affects trafficking by determining the phosphorylation status of KIF3A at serine 690.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PPM1F active protein

Reference Product	PubMed Publications
DIAPH1 active protein	>1 publications with PPM1F and DIAPH1

Diseases associated with PPM1F active protein

Disease Name	Pubmed Publications
Skin Diseases Antibodies	>1 publications with PPM1F and Skin Diseases

Organs/Tissues associated with PPM1F active protein

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>2 publications with PPM1F and Brain
Kidney Antibodies	>1 publications with PPM1F and Kidney