Human Cellexp TFPI2 Active Protein – Cat# MBS844529

TFPI2 Human Cellexp Active Protein

Name: TFPI2 Active Protein; Human Cellexp; MyBioSource
Brand: MyBioSource
SKU: MBS844529
Price: 360 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS844529

Background: Tissue Factor Pathway Inhibitor 2 (TFPI-2), also known as placental protein 5 (PP5) and retinal pigment epithelial cell factor 1 (REF-1), is a member of the Kunitz-type serine proteinase inhibitor family, is a structural homologue of tissue factor… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

TFPI2, Active Protein

Full Product Name

Human CellExp TFPI2, human recombinant

Product Gene Name

TFPI2 active protein

Product Synonym Names

TFPI2; PP5; REF1

Host

HEK293 cells

Form/Format

Lyophilized from 0.22 mum filtered solution in 20 mM MES and 100 mM NaCl, pH 6.5. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Appearance: Lyophilized

Purity/Purification

>=95%

Preparation and Storage

At -20 degree C
Shelf Life: 12 months

Molecular Weight

This protein is fused with 6×His tag at the N-terminus, has a calculated MW of 22.7 kDa. The predicted N-terminus is Asp 23. DTT-reduced Protein migrates as 26-32 kDa due to glycosylation.

OMIM

600033

3D Structure

ModBase 3D Structure for P48307

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Solubility/Reconstitution Instructions

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.

Endotoxin Level

<1 EU/mug by LAL method

Biological Activity

Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca - RPKPVE - Nval - WRK (Dnp) - NH2. The IC50 value is < 2.5 nM, as measured in 100muL reaction mixture containing 1.25 ng trypsin, 10 muM substrate, 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, 0.05% Brij-35, pH 7.5.

Results

Handling

Centrifuge the vial prior to opening.

Product Categories/Family for TFPI2 active protein

Proteins and Enzymes; Human cell expressed recombinant proteinsProteins and Enzymes; Proteins and Enzymes (A-Z) Proteins and Enzymes; Other Proteins & Enzymes

Applications Tested/Suitable for TFPI2 active protein

SDS-PAGE

Other Notes

Small volumes of TFPI2 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TFPI2 purchase

MBS844529 is an active protein targeting TFPI2. Also known as Human CellExp TFPI2, human recombinant. Host: HEK293 cells.

Additional Details

NCBI/Uniprot data below describe general gene information for TFPI2. It may not necessarily be applicable to this product.

NCBI GI #

401871059

NCBI GeneID

7980

NCBI Accession #

NP_001257932.1

UniProt Primary Accession #

P48307

UniProt Secondary Accession #

Q66ME8; Q8NAK6; Q9UC86

UniProt Related Accession #

P48307

GenBank Nucleotide #

NM_001271003.1

NCBI Official Full Name

tissue factor pathway inhibitor 2 isoform 2

NCBI Official Synonym Full Names

tissue factor pathway inhibitor 2

NCBI Official Symbol

TFPI2

NCBI Official Synonym Symbols

PP5; REF1; TFPI-2

UniProt Protein Name

Tissue factor pathway inhibitor 2

UniProt Synonym Protein Names

Placental protein 5; PP5

Protein Family

Tissue factor pathway inhibitor

UniProt Gene Name

TFPI2

UniProt Synonym Gene Names

TFPI-2; PP5

UniProt Entry Name

TFPI2_HUMAN

NCBI Protein Information

tissue factor pathway inhibitor 2

NCBI Summary for TFPI2

This gene encodes a member of the Kunitz-type serine proteinase inhibitor family. The protein can inhibit a variety of serine proteases including factor VIIa/tissue factor, factor Xa, plasmin, trypsin, chymotryspin and plasma kallikrein. This gene has been identified as a tumor suppressor gene in several types of cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

UniProt Comments for TFPI2

TFPI2: May play a role in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa. Has no effect on thrombin.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 7q22

Cellular Component: proteinaceous extracellular matrix; nucleus

Molecular Function: serine-type endopeptidase inhibitor activity; extracellular matrix structural constituent

Biological Process: blood coagulation

Research Articles on TFPI2

1. TMPRSS4 is upregulated by silencing of TFPI-2 through aberrant DNA methylation and contributes to oncogenesis in non-small cell lung cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with TFPI2 active protein

Reference Product	PubMed Publications
TMPRSS4 active protein	>1 publications with TFPI2 and TMPRSS4
IL1B active protein	>1 publications with TFPI2 and IL1B

Diseases associated with TFPI2 active protein

Disease Name	Pubmed Publications
Carcinoma Antibodies	>23 publications with TFPI2 and Carcinoma
Colorectal Neoplasms Antibodies	>13 publications with TFPI2 and Colorectal Neoplasms
Adenocarcinoma Antibodies	>8 publications with TFPI2 and Adenocarcinoma
Inflammation Antibodies	>7 publications with TFPI2 and Inflammation
Neoplasm Metastasis Antibodies	>7 publications with TFPI2 and Neoplasm Metastasis
Pancreatic Neoplasms Antibodies	>6 publications with TFPI2 and Pancreatic Neoplasms
Uterine Cervical Neoplasms Antibodies	>4 publications with TFPI2 and Uterine Cervical Neoplasms
Melanoma Antibodies	>4 publications with TFPI2 and Melanoma
Neoplasms, Experimental Antibodies	>4 publications with TFPI2 and Neoplasms, Experimental
Skin Neoplasms Antibodies	>3 publications with TFPI2 and Skin Neoplasms

Organs/Tissues associated with TFPI2 active protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>15 publications with TFPI2 and Blood
Stomach Antibodies	>6 publications with TFPI2 and Stomach
Liver Antibodies	>5 publications with TFPI2 and Liver
Embryonic Tissue Antibodies	>5 publications with TFPI2 and Embryonic Tissue
Placenta Antibodies	>5 publications with TFPI2 and Placenta
Skin Antibodies	>4 publications with TFPI2 and Skin
Bone Antibodies	>4 publications with TFPI2 and Bone
Uterus Antibodies	>3 publications with TFPI2 and Uterus
Pancreas Antibodies	>3 publications with TFPI2 and Pancreas
Vascular Antibodies	>3 publications with TFPI2 and Vascular