• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Granzyme B (GZMB) Human Cellexp Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS844591

Granzyme B (GZMB) is a serine protease stored in granules of activated cytotoxic T cells and NK cells. Upon target cell contact, granzyme B is directionally exocytosed and assisted by perforin, enters the target cell. With its unique substrate specificity… More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Product Name
Granzyme B (GZMB), Active Protein
Full Product Name
Human CellExp Granzyme B, human recombinant
Product Gene Name
GZMB active protein
Product Synonym Names
GZMB; CCPI; CGL-1; CGL1; CSP-B; CSPB; CTLA1; CTSGL1; HLP; SECT; Fragmentin-2; Granzyme B; Granzyme-2; GRB
Host
HEK 293 cells (Ile 21-Tyr 247)
Form/Format
Lyophilized powder from 0.22 um filtered solution in PBS. containing 8% trehalose
Purity/Purification
> 95% by SDS-PAGE
Specificity
Specific Reactivity > = 5 mU/mg.
Preparation and Storage
Store at -20 °C. After reconstitution, aliquot and store at -20 °C and use within 3 months. Avoid repeated freeze-thaw cycles.
Molecular Weight
The protein is fused with 6×His tag at the C-terminus, has a calculated MW of ~27 kDa. The protein migrates as 35 kDa in SDS-PAGE under reducing conditions due to glycosylation.
3D Structure
ModBase 3D Structure for P10144
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4. Do not vortex. Store the solution in aliquots at -20°C for long term storage.
Unit Definition
One unit of Granzyme B is defined as the amount of enzyme that hydrolyzes 1 umol of Ac-IEPD-AFC per min at 37 °C under the assay conditions (100 uM substrate Ac-IETD-AFC in a buffer containing 50 mM HEPES pH 7.5, 10 mM CaCl2).
Biological Activity
Biological activity was performed using Granzyme B Activity Fluorometric Assay Kit.

Product Categories/Family for GZMB active protein

Cytokines; Growth Factors & Hormones; Human cell expressed recombinant proteinsProteins and Enzymes; Human cell expressed recombinant proteins Proteins and Enzymes; Proteins and Enzymes (A-Z) Apoptosis & Related Products; Non-Caspase Proteases; Granzyme B
Applications Tested/Suitable for GZMB active protein
SDS-PAGE; HPLC
Application Notes for GZMB active protein
Active GZMB is useful in studying enzyme regulation, determining target substrates, screening GZMB inhibitors, or as a positive control in GZMB activity assays

SDS-PAGE of GZMB active protein

Fig A. SDS-PAGE (4-20%) of Recombinant GZMB: Recombinant protein loaded under reducing conditions and stained with Coomassie Blue.
The protein migrates as 35 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Human Cellexp GZMB Active Protein – Cat# MBS844591

Specific activity of GZMB active protein

Fig B. Specific activity: Assay was performed using Granzyme B Activity Fluorometric Assay Kit (Cat #: MBS840001).
The activity of GZMB was determined using 100 uM substrate Ac-IETD-AFC in a buffer containing 50 mM HEPES pH 7.5, 10 mM CaCl2 at 37°C.
Human Cellexp GZMB Active Protein, Specific activity – Cat# MBS844591

Other Notes

Small volumes of GZMB active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GZMB purchase

MBS844591 is an active protein targeting GZMB. Also known as Human CellExp Granzyme B, human recombinant. Host: HEK 293 cells (Ile 21-Tyr 247).

Additional Details

NCBI/Uniprot data below describe general gene information for GZMB. It may not necessarily be applicable to this product.

UniProt Primary Accession #
P10144
Protein Family
Granzyme

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000