DHFR Mouse Recombinant Active Protein

DHFR: Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1. Defects in DHFR are the cause of megaloblastic anemia due to dihydrofolate reductase deficiency (DHFRD). DHFRD is an inborn error of metabolism, characterized by megaloblastic anemia and/or pancytopenia, severe cerebral folate deficiency, and cerebral tetrahydrobiopterin deficiency. Clinical features include variable neurologic symptoms, ranging from severe developmental delay and generalized seizures in infancy, to childhood absence epilepsy with learning difficulties, to lack of symptoms. Belongs to the dihydrofolate reductase family.

Protein type: EC 1.5.1.3; Cofactor and Vitamin Metabolism - one carbon pool by folate; Oxidoreductase; Cofactor and Vitamin Metabolism - folate biosynthesis

Molecular Function: mRNA binding; dihydrofolic acid binding; RNA binding; oxidoreductase activity; drug binding; dihydrofolate reductase activity; NADP binding

Biological Process: tetrahydrofolate metabolic process; tetrahydrofolate biosynthetic process; nucleotide biosynthetic process; dihydrofolate metabolic process; one-carbon compound metabolic process; response to methotrexate; glycine biosynthetic process