BLK Recombinant Protein

Name: BLK Recombinant Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS844619
Price: 430 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS844619

Background: Blk, also known as B lymphoid kinase, is a 55 kd tyrosine kinases with a SH3, SH2 and catalytic domains that contain consensus sequences of the src protein tyrosine kinase family. Blk is expressed specifically in the B cell lineage and plays a… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

BLK, Recombinant Protein

Full Product Name

BLK, Active

Product Gene Name

BLK recombinant protein

Product Synonym Names

BLK; B lymphocyte kinase; p55-Blk ; BLK; B lymphocyte kinase

Host

Baculovirus (Sf9 insect cells)

Form/Format

Recombinant protein in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).
Appearance: Liquid

Purity/Purification

>=90%

Concentration

5ug/50 ul (lot specific)

Preparation and Storage

At -80 degree C
Shelf Life: 12 months

Molecular Weight

84.0 kDa

OMIM

191305

3D Structure

ModBase 3D Structure for P51451

Activity (Specifications/test method)

579 nmol phosphate incorporated into Poly(Glu-Tyr) per minute per mg protein at 30 degree C using a final concentration of 50 uM ATP (0.83 uCi/assay).

Handling

Centrifuge the vial prior to opening.

Applications Tested/Suitable for BLK recombinant protein

SDS-PAGE; HPLC

Other Notes

Small volumes of BLK recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BLK purchase

MBS844619 is a recombinant protein targeting BLK. Also known as BLK, Active. Host: Baculovirus (Sf9 insect cells).

Additional Details

NCBI/Uniprot data below describe general gene information for BLK. It may not necessarily be applicable to this product.

NCBI GI #

33469982

NCBI GeneID

640

NCBI Accession #

NP_001706.2

UniProt Primary Accession #

P51451

UniProt Secondary Accession #

Q16291; Q96IN1

UniProt Related Accession #

P51451

GenBank Nucleotide #

NM_001715.2

NCBI Official Full Name

tyrosine-protein kinase Blk

NCBI Official Synonym Full Names

BLK proto-oncogene, Src family tyrosine kinase

NCBI Official Symbol

BLK

NCBI Official Synonym Symbols

MODY11

UniProt Protein Name

Tyrosine-protein kinase Blk

UniProt Synonym Protein Names

B lymphocyte kinase; p55-Blk

Protein Family

Tyrosine-protein kinase

UniProt Gene Name

BLK

UniProt Entry Name

BLK_HUMAN

NCBI Protein Information

tyrosine-protein kinase Blk

NCBI Summary for BLK

This gene encodes a nonreceptor tyrosine-kinase of the src family of proto-oncogenes that are typically involved in cell proliferation and differentiation. The protein has a role in B-cell receptor signaling and B-cell development. The protein also stimulates insulin synthesis and secretion in response to glucose and enhances the expression of several pancreatic beta-cell transcription factors. [provided by RefSeq, Aug 2010]

UniProt Comments for BLK

BLK: a nonreceptor tyrosine kinase of the Src family. Expressed predominantly in B lymphocytes

Protein type: Protein kinase, tyrosine (non-receptor); Protein kinase, TK; Kinase, protein; EC 2.7.10.2; TK group; Src family

Chromosomal Location of Human Ortholog: 8p23-p22

Cellular Component: extrinsic to internal side of plasma membrane

Molecular Function: protein binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding

Biological Process: B cell receptor signaling pathway; positive regulation of insulin secretion; innate immune response; transmembrane receptor protein tyrosine kinase signaling pathway; T cell differentiation; regulation of cell proliferation

Disease: Maturity-onset Diabetes Of The Young, Type 11

Research Articles on BLK

1. These results place Blk upstream of the p190RhoGAP-RhoA pathway in Galpha13-activated cells, overall representing an opposing signaling module during CXCL12-triggered invasion.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with BLK recombinant protein

Reference Product	PubMed Publications
CD79A recombinant protein	>5 publications with BLK and CD79A
CD79B recombinant protein	>4 publications with BLK and CD79B
EGFR recombinant protein	>3 publications with BLK and EGFR
PLCG2 recombinant protein	>1 publications with BLK and PLCG2
STAT3 recombinant protein	>1 publications with BLK and STAT3
CBL recombinant protein	>1 publications with BLK and CBL

Pathways associated with BLK recombinant protein

Products by Pathway	Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Alpha-synuclein Signaling Pathway antibodies	Alpha-synuclein Signaling Pathway Diagram
Antigen Activates B Cell Receptor (BCR) Leading To Generation Of Second Messengers Pathway antibodies	Antigen Activates B Cell Receptor (BCR) Leading To Generation Of Second Messengers Pathway Diagram
Apoptosis Modulation And Signaling Pathway antibodies	Apoptosis Modulation And Signaling Pathway Diagram
B Cell Receptor Signaling Pathway antibodies	B Cell Receptor Signaling Pathway Diagram
CXCR4-mediated Signaling Events Pathway antibodies	CXCR4-mediated Signaling Events Pathway Diagram
Class I PI3K Signaling Events Pathway antibodies	Class I PI3K Signaling Events Pathway Diagram
EPHA Forward Signaling Pathway antibodies	EPHA Forward Signaling Pathway Diagram
Ephrin B Reverse Signaling Pathway antibodies	Ephrin B Reverse Signaling Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram

Diseases associated with BLK recombinant protein

Disease Name	Pubmed Publications
Immune System Diseases Antibodies	>86 publications with BLK and Immune System Diseases
Lupus Erythematosus, Systemic Antibodies	>50 publications with BLK and Lupus Erythematosus, Systemic
Nervous System Diseases Antibodies	>25 publications with BLK and Nervous System Diseases
Arthritis, Rheumatoid Antibodies	>22 publications with BLK and Arthritis, Rheumatoid
Cardiovascular Diseases Antibodies	>20 publications with BLK and Cardiovascular Diseases
Brain Diseases Antibodies	>19 publications with BLK and Brain Diseases
Inflammation Antibodies	>17 publications with BLK and Inflammation
Necrosis Antibodies	>15 publications with BLK and Necrosis
Neoplasms, Experimental Antibodies	>10 publications with BLK and Neoplasms, Experimental
Lung Diseases Antibodies	>8 publications with BLK and Lung Diseases

Organs/Tissues associated with BLK recombinant protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>148 publications with BLK and Blood
Spleen Antibodies	>16 publications with BLK and Spleen
Lung Antibodies	>15 publications with BLK and Lung
Bone Marrow Antibodies	>14 publications with BLK and Bone Marrow
Lymph Node Antibodies	>10 publications with BLK and Lymph Node
Lymph Antibodies	>10 publications with BLK and Lymph
Intestine Antibodies	>5 publications with BLK and Intestine
Thymus Antibodies	>5 publications with BLK and Thymus
Connective Tissue Antibodies	>3 publications with BLK and Connective Tissue
Mammary Gland Antibodies	>3 publications with BLK and Mammary Gland