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Bacterial Recombinant MDH Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS844626

Background: Malate dehydrogenase (MDH) belongs to the LDH/MDH superfamily and MDH type 1 family. This enzyme catalyzes the conversion of malate into oxaloacetate (using NAD+) and vice versa (reversible reaction). This reaction is part of many metabolic… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
Bacterial Recombinant MDH, Active Protein
Full Product Name
Bacterial Recombinant MDH
Product Synonym Names
Malate dehydrogenase; ECK3225; JW3205; Bacterial Recombinant MDH
Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMKVAVL GAAGGIGQAL ALLLKTQLPS GSELSLYDIA PVTPGVAVDL SHIPTAVKIK GFSGEDATPA LEGADVVLIS AGVARKPGMD RSDLFNVNAG IVKNLVQQVA KTCPKACIGI ITNPVNTTVA IAAEVLKKAG VYDKNKLFGV TTLDIIRSNT FVAELKGKQP GEVEVPVIGG HSGVTILPLL SQVPGVSFTE QEVADLTKRI QNAGTEVVEA KAGGGSATLS MGQAAARFGL SLVRALQGEQ GVVECAYVEG DGQYARFFSQ PLLLGKNGVE ERKSIGTLSA FEQNALEGML DTLKKDIALG EEFVNK
Host
E Coli
Form/Format
1 mg/ml solution in 20 mM Tris-HCl (pH 8.0) containing 1 mM DTT, 50 mM NaCl and 10% glycerol.
Appearance: Liquid
Purity/Purification
>=95%
Concentration
1 mg/ml (lot specific)
Preparation and Storage
At -80 degree C
Shelf Life: 12 months
Molecular Weight
34.9 kDa (336 aa, 1-312 aa + NT His Tag)
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Biological Activity
Specific activity is > 450 units/mg, and is defined as the amount of enzyme that cleaves 1 umole of oxaloacetate and beta-NADH to L-malate and beta-NAD per minute at pH 7.5 at 25 degree C.
Unit Definition
Specific activity is defined as the amount of enzyme that cleaves 1 umole of oxaloacetate and beta-NADH to L-malate and beta-NAD per minute at pH 7.5 at 25 degree C.
Handling
Centrifuge the vial prior to opening.
Applications Tested/Suitable for Bacterial Recombinant MDH active protein
SDS-PAGE

Other Notes

Small volumes of Bacterial Recombinant MDH active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Bacterial Recombinant MDH purchase

MBS844626 is an active protein targeting Bacterial Recombinant MDH. Host: E Coli.
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