Flt3-Ligand (FLT3-L) Active Protein

Name: FLT3-L Active Protein; Flt3-Ligand; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS844765
Price: 315 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS844765

Background: Human flt3-Ligand (flk2-ligand) stimulates the proliferation and colony formation of certain bone marrow precursor cells including CD34+ cells. Recombinant human flt3-ligand is a soluble 17.6 kDa polypeptide containing 155 amino acid residues,… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

flt3-Ligand, Active Protein

Full Product Name

flt3-Ligand, human recombinant

Product Gene Name

Flt3-L active protein

Product Synonym Names

Fms-related tyrosine kinase 3 ligand; Short name=Flt3 ligand; Short name=Flt3L; SL cytokine

Host

E Coli

Form/Format

Sterile filtered and lyophilized with no additives. The protein may appear as a haze of film, which is difficult to see at the bottom of the vial.
Appearance: Lyophilized protein

Purity/Purification

>=98%

Preparation and Storage

At -20 degree C
Shelf Life: 12 months

Molecular Weight

17.6 kDa

OMIM

600007

3D Structure

ModBase 3D Structure for P49771

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Solubility/Reconstitution Instructions

Reconstitute in H?O to a concentration of 0.1 - 1.0 mg/ml. The solution can then be diluted into other aqueous buffers and stored at 4 degree C for 1 week or -20 degree C for future use.

Endotoxin Level

<0.1 ng/mug

Biological Activity

The ED?? as determined by the dose-dependent stimulation of the proliferation of human AML5 cells is <1.0 ng/ml, corresponding to a specific activity of > 1 x 10 units/mg.

Results

<1.0 ng/ml

Handling

Centrifuge the vial prior to opening.

Applications Tested/Suitable for Flt3-L active protein

SDS-PAGE; HPLC

Other Notes

Small volumes of Flt3-L active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Flt3-L purchase

MBS844765 is an active protein targeting Flt3-L. Also known as flt3-Ligand, human recombinant. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for Flt3-L. It may not necessarily be applicable to this product.

NCBI GI #

325197197

NCBI GeneID

2323

NCBI Accession #

NP_001191431.1

UniProt Primary Accession #

P49771

UniProt Secondary Accession #

Q05C96; A0AVC2; B9EGH2

UniProt Related Accession #

P49771

GenBank Nucleotide #

NM_001204502.1

NCBI Official Full Name

fms-related tyrosine kinase 3 ligand isoform 1

NCBI Official Synonym Full Names

fms-related tyrosine kinase 3 ligand

NCBI Official Symbol

FLT3LG

NCBI Official Synonym Symbols

FL; FLT3L

UniProt Protein Name

Fms-related tyrosine kinase 3 ligand

UniProt Synonym Protein Names

SL cytokine

Protein Family

Fms-related tyrosine kinase

UniProt Gene Name

FLT3LG

UniProt Synonym Gene Names

Flt3 ligand; Flt3L

UniProt Entry Name

FLT3L_HUMAN

NCBI Protein Information

fms-related tyrosine kinase 3 ligand

NCBI Summary for Flt3-L

Dendritic cells (DCs) provide the key link between innate and adaptive immunity by recognizing pathogens and priming pathogen-specific immune responses. FLT3LG controls the development of DCs and is particularly important for plasmacytoid DCs and CD8 (see MIM 186910)-positive classical DCs and their CD103 (ITGAE; MIM 604682)-positive tissue counterparts (summary by Sathaliyawala et al., 2010 [PubMed 20933441]).[supplied by OMIM, Jan 2011]

UniProt Comments for Flt3-L

FLT3LG: Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligand, receptor tyrosine kinase; Cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: extracellular space; membrane; integral to membrane

Molecular Function: protein homodimerization activity; cytokine activity; receptor tyrosine kinase binding; receptor binding

Biological Process: positive regulation of natural killer cell differentiation; regulation of myeloid dendritic cell activation; lymphocyte differentiation; embryonic hemopoiesis; positive regulation of cell proliferation; positive regulation of protein amino acid phosphorylation; signal transduction; positive regulation of myoblast differentiation

Research Articles on Flt3-L

1. The immunohistochemical expression of caveolin-1 and podocalyxin in lungs from rats challenged with a 2-kDa macrophage-activating lipopeptide (MALP-2) and Flt3L, was examined.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with Flt3-L active protein

Reference Product	PubMed Publications
FLT3 active protein	>6 publications with Flt3-L and FLT3
CD34 active protein	>4 publications with Flt3-L and CD34
KITLG active protein	>4 publications with Flt3-L and KITLG
IL3 active protein	>2 publications with Flt3-L and IL3
THPO active protein	>2 publications with Flt3-L and THPO

Diseases associated with Flt3-L active protein

Disease Name	Pubmed Publications
Lymphoma Antibodies	>2 publications with Flt3-L and Lymphoma
Leukemia Antibodies	>2 publications with Flt3-L and Leukemia
Chromosome Aberrations Antibodies	>1 publications with Flt3-L and Chromosome Aberrations
Inflammation Antibodies	>1 publications with Flt3-L and Inflammation

Organs/Tissues associated with Flt3-L active protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>5 publications with Flt3-L and Blood
Pancreas Antibodies	>1 publications with Flt3-L and Pancreas