Pd-L1/CD274/B7-H1 (CD274) Active Protein

Name: CD274 Active Protein; Pd-L1/CD274/B7-H1; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS844786
Price: 290 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS844786

Background: Programmed cell death 1 ligand 1 (PD-L1) is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

PD-L1/CD274/B7-H1, Active Protein

Full Product Name

Human CellExp PD-L1 / CD274 / B7-H1, human recombinant

Product Gene Name

CD274 active protein

Product Synonym Names

PD-L1; CD274; B7-H1; PDCD1L1; PDCD1LG1

Host

HEK293 cells

Form/Format

Lyophilized from 0.22 mum filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Appearance: Lyophilized

Purity/Purification

>=95%

Preparation and Storage

At -20 degree C
Shelf Life: 12 months

Molecular Weight

This protein is fused with polyhistidine tag at the C-terminus, has a calculated MW of 26 kDa. The predicted N-terminus is Phe 19. DTT-reduced Protein migrates as 35-42 kDa due to glycosylation.

OMIM

605402

3D Structure

ModBase 3D Structure for Q9NZQ7

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Solubility/Reconstitution Instructions

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.

Endotoxin Level

<1 EU/mug by LAL method

Biological Activity

Measured by its binding ability in a functional ELISA. Immobilized rh PD-L1 / B7-H1 at 2 mug/ml (100 mul/well) can bind rhPD1/PDCD1 Fc Chimera with a linear range of 1-10 mug/ml. Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is typically 0.6-5 mug/ml.

Results

Handling

Centrifuge the vial prior to opening.

Product Categories/Family for CD274 active protein

Cytokines; Growth Factors & Hormones; Human cell expressed recombinant proteins Proteins and Enzymes; Human cell expressed recombinant proteinsProteins and Enzymes; Cytokines; Growth Factors & Hormones; CDsProteins and Enzymes; Cytokines; Growth Factors &

Applications Tested/Suitable for CD274 active protein

SDS-PAGE

Other Notes

Small volumes of CD274 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD274 purchase

MBS844786 is an active protein targeting CD274. Also known as Human CellExp PD-L1 / CD274 / B7-H1, human recombinant. Host: HEK293 cells.

Additional Details

NCBI/Uniprot data below describe general gene information for CD274. It may not necessarily be applicable to this product.

NCBI GI #

390979639

NCBI GeneID

29126

NCBI Accession #

NP_001254635.1

UniProt Primary Accession #

Q9NZQ7

UniProt Secondary Accession #

Q14CJ2; Q2V8D5; Q66RK1; Q6WEX4; Q9NUZ5; B2RBA2; B4DU27

UniProt Related Accession #

Q9NZQ7

GenBank Nucleotide #

NM_001267706.1

NCBI Official Full Name

programmed cell death 1 ligand 1 isoform b

NCBI Official Synonym Full Names

CD274 molecule

NCBI Official Symbol

CD274

NCBI Official Synonym Symbols

B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1

UniProt Protein Name

Programmed cell death 1 ligand 1

UniProt Synonym Protein Names

B7 homolog 1; B7-H1; CD_antigen: CD274

Protein Family

Programmed cell death

UniProt Gene Name

CD274

UniProt Synonym Gene Names

B7H1; PDCD1L1; PDCD1LG1; PDL1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7-H1

UniProt Entry Name

PD1L1_HUMAN

NCBI Protein Information

programmed cell death 1 ligand 1

UniProt Comments for CD274

CD274: Involved in the costimulatory signal, essential for T- cell proliferation and production of IL10 and IFNG, in an IL2- dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. Belongs to the immunoglobulin superfamily. BTN/MOG family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 9p24

Cellular Component: integral to membrane; plasma membrane; endomembrane system; external side of plasma membrane

Molecular Function: protein binding

Biological Process: regulation of activated T cell proliferation; cell surface receptor linked signal transduction; negative regulation of interleukin-10 production; negative regulation of interferon-gamma production; negative regulation of activated T cell proliferation; T cell costimulation; positive regulation of T cell proliferation; immune response; signal transduction

Research Articles on CD274

1. genetic association studies in population in Chile: Data suggest that SNPs in PDL1 (rs2297137; rs4143815) but not PDL2 are associated with type 1 diabetes in the population studied; PDL1 serum levels are down-regulated in type 1 diabetes.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CD274 active protein

Reference Product	PubMed Publications
CD80 active protein	>399 publications with CD274 and CD80
PDCD1 active protein	>364 publications with CD274 and PDCD1
PDCD1LG2 active protein	>175 publications with CD274 and PDCD1LG2
LCK active protein	>1 publications with CD274 and LCK

Pathways associated with CD274 active protein

Products by Pathway	Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Costimulation By The CD28 Family Pathway antibodies	Costimulation By The CD28 Family Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
PD-1 Signaling Pathway antibodies	PD-1 Signaling Pathway Diagram

Diseases associated with CD274 active protein

Disease Name	Pubmed Publications
Inflammation Antibodies	>124 publications with CD274 and Inflammation
Necrosis Antibodies	>80 publications with CD274 and Necrosis
Neoplasms, Experimental Antibodies	>76 publications with CD274 and Neoplasms, Experimental
Adenocarcinoma Antibodies	>76 publications with CD274 and Adenocarcinoma
Nervous System Diseases Antibodies	>75 publications with CD274 and Nervous System Diseases
Lung Neoplasms Antibodies	>62 publications with CD274 and Lung Neoplasms
Lung Diseases Antibodies	>61 publications with CD274 and Lung Diseases
Kidney Diseases Antibodies	>54 publications with CD274 and Kidney Diseases
Recurrence Antibodies	>37 publications with CD274 and Recurrence
Neoplasm Metastasis Antibodies	>36 publications with CD274 and Neoplasm Metastasis

Organs/Tissues associated with CD274 active protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>838 publications with CD274 and Blood
Lung Antibodies	>95 publications with CD274 and Lung
Lymph Node Antibodies	>81 publications with CD274 and Lymph Node
Bone Antibodies	>72 publications with CD274 and Bone
Skin Antibodies	>49 publications with CD274 and Skin
Brain Antibodies	>36 publications with CD274 and Brain
Pancreas Antibodies	>29 publications with CD274 and Pancreas
Placenta Antibodies	>19 publications with CD274 and Placenta
Stomach Antibodies	>13 publications with CD274 and Stomach
Mouth Antibodies	>9 publications with CD274 and Mouth