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Procathepsin S (CTSS) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS844822

Background: Cathepsin S (CTSS) is a lysosomal cysteine protease of the papain family and may participate in the degradation of antigenic proteins to peptides for presentation on MHC class II molecules. CTSS is synthesized as inactive precursor of 331 amino… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
Procathepsin S (CTSS), Active Protein
Full Product Name
Procathepsin S, human recombinant
Product Gene Name
CTSS active protein
Product Synonym Names
CTSS
Host
E Coli
Form/Format
Lyophilized from proprietary buffer.
Appearance: Lyophilized
Purity/Purification
>=90%
Preparation and Storage
At -80 degree C
Shelf Life: 12 months
Molecular Weight
38.1 kDa (17-331 aa + N-terminal poly-his tag)
OMIM
116845
3D Structure
ModBase 3D Structure for P25774
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Solubility/Reconstitution Instructions
Reconstitute in 25 mM Na2HPO4, 500 mM NaCl (pH 7.0) to 0.5-1 mg/ml
Biological Activity
MyBioSource's Procathepsin S can be auto-catalytically activated to mature Cathepsin S (~24 kDa) by adding an equal volume of 0.2 M NaOAc, 5 mM EDTA pH 4.0 containing fresh 5 mM DTT at room temperature (RT). The enzyme is fully activated within 2-3 hrs. of incubation at RT. After activation mature Cathepsin S is highly auto-proteolytic and care must be taken to avoid self-proteolysis.
Results
>1000 mU/mg (1 U = Digestion of 1 umole/min) of Ac-VVR-AFC substrate (K144-100) after full activation.
Unit Definition
1 U = Digestion of 1 umole/min
Handling
Centrifuge the vial prior to opening.

Product Categories/Family for CTSS active protein

Apoptosis & Related Products; Non-Caspase Proteases; Cathepsin; Cathepsin EnzymesProteins and Enzymes; Proteases; CathepsinsProteins and Enzymes; Animal-Free Origin (AOF) Recombinant Proteins & EnzymesProteins and Enzymes; Proteins and Enzymes (A-Z)
Applications Tested/Suitable for CTSS active protein
SDS-PAGE

Testing Data of CTSS active protein

CTSS Active Protein – Cat# MBS844822

Other Notes

Small volumes of CTSS active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CTSS purchase

MBS844822 is an active protein targeting CTSS. Also known as Procathepsin S, human recombinant. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for CTSS. It may not necessarily be applicable to this product.

NCBI GI #
315075311
NCBI GeneID
1520
NCBI Accession #
NP_001186668.1
UniProt Primary Accession #
P25774
UniProt Secondary Accession #
Q5T5I0; Q6FHS5; Q9BUG3; B4DWC9; D3DV05
UniProt Related Accession #
P25774
GenBank Nucleotide #
NM_001199739.1
NCBI Official Full Name
cathepsin S isoform 2 preproprotein
NCBI Official Synonym Full Names
cathepsin S
NCBI Official Symbol
CTSS
UniProt Protein Name
Cathepsin S
Protein Family
Cathepsin
UniProt Gene Name
CTSS
UniProt Entry Name
CATS_HUMAN
NCBI Protein Information
cathepsin S

NCBI Summary for CTSS

The protein encoded by this gene, a member of the peptidase C1 family, is a lysosomal cysteine proteinase that may participate in the degradation of antigenic proteins to peptides for presentation on MHC class II molecules. The encoded protein can function as an elastase over a broad pH range in alveolar macrophages. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2010]
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UniProt Comments for CTSS

CTSS: Thiol protease. Key protease responsible for the removal of the invariant chain from MHC class II molecules. The bond- specificity of this proteinase is in part similar to the specificities of cathepsin L and cathepsin N. Belongs to the peptidase C1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.22.27; Protease

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: extracellular space; lysosomal lumen; membrane; intracellular membrane-bound organelle; lysosome; extracellular region

Molecular Function: collagen binding; proteoglycan binding; cysteine-type endopeptidase activity; fibronectin binding; laminin binding

Biological Process: extracellular matrix organization and biogenesis; adaptive immune response; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; antigen processing and presentation of exogenous peptide antigen via MHC class II; proteolysis; collagen catabolic process; antigen processing and presentation; extracellular matrix disassembly; antigen processing and presentation of peptide antigen via MHC class I; proteolysis involved in cellular protein catabolic process; toll-like receptor signaling pathway; innate immune response; antigen processing and presentation of exogenous peptide antigen via MHC class I; immune response; positive regulation of inflammatory response
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Research Articles on CTSS

1. Increased plasma CTSS concentration is associated with atherogenesis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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