Fetuin A/ASHG (FETUA) Active Protein

Name: FETUA Active Protein; Fetuin A/ASHG; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS844824
Price: 220 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS844824

Background: Fetuin A/AHSG is a human plasma glycoprotein belonging to the Cystatin family of proteases inhibitors. It is highly expressed in embryonic cells and adult hepatocytes, and is expressed to a lesser extent in monocytes/macrophages. Fetuin A/AHSG is… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

Fetuin A/ASHG (FETUA), Active Protein

Full Product Name

Human CellExp Fetuin A / ASHG, Human Recombinant

Product Gene Name

FETUA active protein

Product Synonym Names

Alpha-2-HS-glycoprotein; Alpha-2-Z-globulin; Ba-alpha-2-glycoprotein

Sequence

A-Chain: APHGPGLIYR QPNCDDPETE EAALVAIDYI NQNLPWGYKH TLNQIDEVKV WPQQPSGELF EIEIDTLETT CHVLDPTPVA RCSVRQLKEH AVEGDCDFQL LKLDGKFSVV YAKCDSSPDS AEDVRKVCQD CPLLAPLNDT RVVHAAKAAL AAFNAQNNGS NFQLEEISRA QLVPLPPSTY VEFTVSGTDC VAKEATEAAK CNLLAEKQYG FCKATLSEKL GGAEVAVTCT VFQTQPVTSQ PQPEGANEAV PTPVVDPDAP PSPPLGAPGL PPAGSPPDSH VL B-Chain: TVVQPSVGAA AGPVVPPCPG RIRHFKV

Host

HEK 293 cells

Form/Format

Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.8.
Appearance: Lyophilized powder

Purity/Purification

>=95%

Preparation and Storage

At -20 degree C
Shelf Life: 12 months

Molecular Weight

45-55 kDa

OMIM

138680

3D Structure

ModBase 3D Structure for P02765

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Solubility/Reconstitution Instructions

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20 degree C to -80 degree C.

Endotoxin Level

< 0.1 ng/mug of protein (<1EU/mug).

Activity (Specifications/test method)

Determined by its ability to inhibit Cathepsin V cleavage of a fluorogenic peptide substrate ZLR-AMC (RLUs). The expected IC50 is <= 100nM.

Biological Activity

Determined by its ability to inhibit Cathepsin V cleavage of a fluorogenic peptide substrate ZLR-AMC (RLUs). The expected IC50 is <= 100nM.

Handling

Centrifuge the vial prior to opening.

Product Categories/Family for FETUA active protein

Cytokines; Growth Factors & Hormones; Human cell expressed recombinant proteins Proteins and Enzymes; Human cell expressed recombinant proteins Cytokines; Growth Factors & Hormones; TGFsCytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A-Z

Applications Tested/Suitable for FETUA active protein

SDS-PAGE; HPLC

Other Notes

Small volumes of FETUA active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FETUA purchase

MBS844824 is an active protein targeting FETUA. Also known as Human CellExp Fetuin A / ASHG, Human Recombinant. Host: HEK 293 cells.

Additional Details

NCBI/Uniprot data below describe general gene information for FETUA. It may not necessarily be applicable to this product.

NCBI GI #

156523970

NCBI GeneID

197

NCBI Accession #

NP_001613.2

UniProt Primary Accession #

P02765

UniProt Secondary Accession #

O14961; O14962; Q9P152; A8K9N6; B2R7G1

UniProt Related Accession #

P02765; AAB29984

GenBank Nucleotide #

NM_001622.2

NCBI Official Full Name

alpha-2-HS-glycoprotein preproprotein

NCBI Official Synonym Full Names

alpha-2-HS-glycoprotein

NCBI Official Symbol

AHSG

NCBI Official Synonym Symbols

AHS; A2HS; HSGA; FETUA

UniProt Protein Name

Alpha-2-HS-glycoprotein

UniProt Synonym Protein Names

Alpha-2-Z-globulin; Ba-alpha-2-glycoprotein; Fetuin-A

UniProt Gene Name

AHSG

UniProt Synonym Gene Names

FETUA

UniProt Entry Name

FETUA_HUMAN

NCBI Protein Information

alpha-2-HS-glycoprotein

NCBI Summary for FETUA

Alpha2-HS glycoprotein (AHSG), a glycoprotein present in the serum, is synthesized by hepatocytes. The AHSG molecule consists of two polypeptide chains, which are both cleaved from a proprotein encoded from a single mRNA. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix, and it has therefore been postulated that it participates in the development of the tissues. However, its exact significance is still obscure. [provided by RefSeq, Jul 2008]

UniProt Comments for FETUA

FETUA: Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions. Alpha-2-HS glycoprotein derives from this precursor, when the connecting peptide is cleaved off. The two chains A and B are held together by a single disulfide bond. Synthesized in liver and selectively concentrated in bone matrix. Secreted in plasma. It is also found in dentin in much higher quantities than other plasma proteins. Belongs to the fetuin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3q27

Cellular Component: extracellular space; extracellular region

Molecular Function: kinase inhibitor activity; cysteine protease inhibitor activity

Biological Process: pinocytosis; negative regulation of bone mineralization; ossification; positive regulation of phagocytosis; regulation of inflammatory response; acute-phase response; negative regulation of phosphorylation; negative regulation of insulin receptor signaling pathway; regulation of bone mineralization; skeletal development

Research Articles on FETUA

1. Serum Fetuin-A level is closely correlated with osteoporosis and it may serve as a predictor of osteoporosis in maintenance hemodialysis patients

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.