ZAP70 Recombinant Protein

Name: ZAP70 Recombinant Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS844896
Price: 430 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS844896

Background: ZAP70 is a non-receptor protein tyrosine kinase (part of the Syk/Zap70 family) that is involved in signaling by the T-cell antigen receptor (TCR). Ligation of the TCR/CD3 receptor in Jurkat T-cells induces phosphoprotein complexes which contain… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

ZAP70, Recombinant Protein

Full Product Name

ZAP70, Active

Product Gene Name

ZAP70 recombinant protein

Product Synonym Names

ZAP70; zeta-chain (TCR) associated protein kinase 70kDa; Syk-related tyrosine kinase

Host

Baculovirus (Sf9 insect cells)

Form/Format

Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).
Appearance: Liquid

Purity/Purification

>=90%

Concentration

5ug/50 ul (lot specific)

Preparation and Storage

At -80 degree C
Shelf Life: 12 months

Molecular Weight

96.0 kDa

OMIM

176947

3D Structure

ModBase 3D Structure for P43403

Activity (Specifications/test method)

113 nmol phosphate incorporated into Poly(Glu-Tyr) substrate per minute per mg protein at 30 degree C for 15 minutes using a final concentration of 50 uM ATP (0.83 uCi/assay in 25ul reaction volume).

Handling

Centrifuge the vial prior to opening.

Applications Tested/Suitable for ZAP70 recombinant protein

SDS-PAGE; HPLC

Other Notes

Small volumes of ZAP70 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ZAP70 purchase

MBS844896 is a recombinant protein targeting ZAP70. Also known as ZAP70, Active. Host: Baculovirus (Sf9 insect cells).

Additional Details

NCBI/Uniprot data below describe general gene information for ZAP70. It may not necessarily be applicable to this product.

NCBI GI #

31455611

NCBI GeneID

7535

NCBI Accession #

NP_001070.2

UniProt Primary Accession #

P43403

UniProt Secondary Accession #

Q6PIA4; Q8IXD6; Q9UBS6; A6NFP4

UniProt Related Accession #

P43403

GenBank Nucleotide #

NM_001079.3

NCBI Official Full Name

tyrosine-protein kinase ZAP-70 isoform 1

NCBI Official Synonym Full Names

zeta-chain (TCR) associated protein kinase 70kDa

NCBI Official Symbol

ZAP70

NCBI Official Synonym Symbols

SRK; STD; TZK; STCD; ZAP-70

UniProt Protein Name

Tyrosine-protein kinase ZAP-70

UniProt Synonym Protein Names

70 kDa zeta-chain associated protein; Syk-related tyrosine kinase

Protein Family

Tyrosine-protein kinase

UniProt Gene Name

ZAP70

UniProt Synonym Gene Names

SRK

UniProt Entry Name

ZAP70_HUMAN

NCBI Protein Information

tyrosine-protein kinase ZAP-70

NCBI Summary for ZAP70

This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

UniProt Comments for ZAP70

ZAP70: a tyrosine kinase of the Syk family. Associates with the T-cell antigen receptor zeta-chain after TCR stimulation. Phosphorylated by Src-family kinases following antigen receptor activation. Plays a role in lymphocyte activation.

Protein type: Kinase, protein; Protein kinase, tyrosine (non-receptor); Protein kinase, TK; EC 2.7.10.2; TK group; Syk family

Chromosomal Location of Human Ortholog: 2q12

Cellular Component: T cell receptor complex; extrinsic to internal side of plasma membrane; cytoplasm; plasma membrane; immunological synapse; intercellular junction; cytosol; lipid raft

Molecular Function: protein binding; phosphotyrosine binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding

Biological Process: adaptive immune response; cell migration; peptidyl-tyrosine phosphorylation; B cell activation; T cell activation; positive regulation of calcium-mediated signaling; negative thymic T cell selection; T cell receptor signaling pathway; protein amino acid phosphorylation; positive regulation of T cell differentiation; innate immune response; immune response; beta selection; positive regulation of alpha-beta T cell proliferation; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of alpha-beta T cell differentiation; positive thymic T cell selection; T cell differentiation

Disease: Selective T-cell Defect

Research Articles on ZAP70

1. novel biosensor ROZA-XL displays a 3-4 times greater dynamic range than its predecessor and possesses a robust baseline FRET value for ZAP-70

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.