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Influenza A Virus/Neuraminidase (Na) (neuraminidase/NA) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS844917

Background: Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
Influenza A virus/Neuraminidase (NA) (neuraminidase/NA), Active Protein
Full Product Name
Human CellExp Influenza A virus / Neuraminidase (NA)
Product Synonym Names
NA; Neuraminidase
Host
HEK293 cells
Form/Format
Lyophilized from 0.22 mum filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Appearance: Lyophilized
Purity/Purification
>=92%
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Molecular Weight
Influenza A virus (A/Thailand/1(KAN-1)/2004 (H5N1)) Neuraminidase (NA) is fused with a polyhistidine tag at the N-terminus, and has a calculated MW of 46.1 kDa. The predicted N-terminus is His 36. DTT-reduced Protein migrates as 48 kDa in SDS-PAGE
3D Structure
ModBase 3D Structure for Q76UU8
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Solubility/Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.
Endotoxin Level
<1 EU/mug by LAL method
Biological Activity
Measured by its ability to cleave a fluorogenic substrate, 2'-(4-Methylumbelliferyl)-alpha-D-N-acetylneuraminic acid. One unit is defined as the amount of enzyme required to cleave 1 nmole of 2'-(4-Methylumbelliferyl)-alpha-D-N-acetylneuraminic acid per minute at pH 7.5 at 37 degree C
Results
Measured by its ability to cleave a fluorogenic substrate, 2'-(4-Methylumbelliferyl)-alpha-D-N-acetylneuraminic acid. One unit is defined as the amount of enzyme required to cleave 1 nmole of 2'-(4-Methylumbelliferyl)-alpha-D-N-acetylneuraminic acid per minute at pH 7.5 at 37 degree C
Unit Definition
One unit is defined as the amount of enzyme required to cleave 1 nmole of 2'-(4-Methylumbelliferyl)-alpha-D-N-acetylneuraminic acid per minute at pH 7.5 at 37 degree C
Handling
Centrifuge the vial prior to opening.
Applications Tested/Suitable for neuraminidase/NA active protein
SDS-PAGE

Other Notes

Small volumes of neuraminidase/NA active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for neuraminidase/NA purchase

MBS844917 is an active protein targeting neuraminidase/NA. Also known as Human CellExp Influenza A virus / Neuraminidase (NA). Host: HEK293 cells.

Additional Details

NCBI/Uniprot data below describe general gene information for neuraminidase/NA. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
UniProt Primary Accession #
NCBI Official Full Name
Neuraminidase
UniProt Protein Name
Neuraminidase
UniProt Gene Name
neuraminidase/NA
UniProt Entry Name
Q76UU8_9INFB

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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