Il-21 (IL21) Active Protein

Name: IL21 Active Protein; Il-21; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS844935
Price: 315 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS844935

Background: Mouse Interleukin-21 (m-IL-21), expressed by activated CD4(+) T cells, is the newest member of the common gamma-chain family of cytokines, which also includes IL-2, IL-4, IL-7, IL-9, and IL-13. Its private receptor, IL-21R, is expressed on T, B,… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

IL-21 (IL21), Active Protein

Full Product Name

IL-21, murine recombinant

Product Gene Name

IL21 active protein

Product Synonym Names

Za11; IL-21; Interleukin-21; IL-21; Interleukin 21

Host

E Coli

Form/Format

Lyophilized from 20 mM NaHCO?, pH 8.5
Appearance: Lyophilized protein

Purity/Purification

>=95%

Preparation and Storage

At -20 degree C
Shelf Life: 12 months

Molecular Weight

15.095 kDa

3D Structure

ModBase 3D Structure for Q9ES17

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Solubility/Reconstitution Instructions

When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile H?O to a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.

Endotoxin Level

<0.01 ng/mug

Biological Activity

The bioactivity is determined by a dose-dependent proliferation of lymphocytes co-stimulated with anti-CD3? and is typically 2-50 ng/ml.

Handling

Centrifuge the vial prior to opening.

Product Categories/Family for IL21 active protein

Proteins and Enzymes; Cytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A-Z) Cytokines; Growth Factors & Hormones; Interleukins

Applications Tested/Suitable for IL21 active protein

SDS-PAGE; HPLC

Other Notes

Small volumes of IL21 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL21 purchase

MBS844935 is an active protein targeting IL21. Also known as IL-21, murine recombinant. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for IL21. It may not necessarily be applicable to this product.

NCBI GI #

11140821

NCBI GeneID

60505

NCBI Accession #

NP_068554.1

UniProt Primary Accession #

Q9ES17

UniProt Related Accession #

Q9ES17

GenBank Nucleotide #

NM_021782.3

NCBI Official Full Name

interleukin-21 isoform 2

NCBI Official Synonym Full Names

interleukin 21

NCBI Official Symbol

Il21

NCBI Official Synonym Symbols

IL-21

UniProt Protein Name

Interleukin-21

Protein Family

Interleukin

UniProt Gene Name

Il21

UniProt Synonym Gene Names

IL-21

UniProt Entry Name

IL21_MOUSE

NCBI Protein Information

interleukin-21

UniProt Comments for IL21

IL21: Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells. May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation. Belongs to the IL-15/IL-21 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Cytokine; Apoptosis; Cell development/differentiation; Secreted

Cellular Component: extracellular space; extracellular region

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor binding; interleukin-2 receptor binding; cytokine activity

Biological Process: positive regulation of interleukin-17 production; positive regulation of natural killer cell cytokine production; cell maturation; B cell apoptosis; positive regulation of natural killer cell mediated cytotoxicity; positive regulation of interleukin-10 production; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of natural killer cell differentiation; positive regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of cell proliferation; positive regulation of B cell proliferation; positive regulation of B cell differentiation; positive regulation of T cell proliferation; immune response; positive regulation of interferon-gamma biosynthetic process

Research Articles on IL21

1. Data indicate that interleukin 21 knockout (il-21-/-) mice showed significantly diminished T follicular helper cell (TFH) generation/accumulation compared to wild type mice.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.