MMP-9 (MMP4) Active Protein

Name: MMP4 Active Protein; MMP-9; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS844946
Price: 265 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS844946

Background: MMP-9 catalytic domain from human cDNA, expressed in E.coli and purified using proprietary technologies. The MMP-9 is useful as a standard in Western blot, etc. More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

MMP-9 (MMP4), Active Protein

Full Product Name

MMP-9, human recombinant

Product Gene Name

MMP4 active protein

Product Synonym Names

Matrix metalloproteinase-9 MMP-9; 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELB; Cleaved into: 67 kDa matrix metalloproteinase-9; 82 kDa matrix metalloproteinase-9

Host

E Coli

Form/Format

Lyophilized with no additives
Appearance: Lyophilized powder

Purity/Purification

>=95%

Preparation and Storage

At -20 degree C
Shelf Life: 12 months

Molecular Weight

39.0 kDa

OMIM

120361

3D Structure

ModBase 3D Structure for P14780

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Solubility/Reconstitution Instructions

Reconstitute in sterile PBS to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4 degree C for 1 week or -20 degree C for future use.

Endotoxin Level

<0.1 ng/mug

Biological Activity

Biological activity of the protein has not been determined.

Handling

Centrifuge the vial prior to opening.

Applications Tested/Suitable for MMP4 active protein

SDS-PAGE; HPLC

Other Notes

Small volumes of MMP4 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MMP4 purchase

MBS844946 is an active protein targeting MMP4. Also known as MMP-9, human recombinant. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for MMP4. It may not necessarily be applicable to this product.

NCBI GI #

74272287

NCBI GeneID

4318

NCBI Accession #

NP_004985.2

UniProt Primary Accession #

P14780

UniProt Secondary Accession #

Q3LR70; Q8N725; Q9H4Z1; Q9UCJ9; Q9UCL1; Q9UDK2; B2R7V9

UniProt Related Accession #

P14780

GenBank Nucleotide #

NM_004994.2

NCBI Official Full Name

matrix metalloproteinase-9 preproprotein

NCBI Official Synonym Full Names

matrix metallopeptidase 9

NCBI Official Symbol

MMP9

NCBI Official Synonym Symbols

GELB; CLG4B; MMP-9; MANDP2

UniProt Protein Name

Matrix metalloproteinase-9

UniProt Synonym Protein Names

92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELB

Protein Family

Matrix metalloproteinase

UniProt Gene Name

MMP9

UniProt Synonym Gene Names

CLG4B; MMP-9; GELB

UniProt Entry Name

MMP9_HUMAN

NCBI Protein Information

matrix metalloproteinase-9

NCBI Summary for MMP4

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq, Jul 2008]

UniProt Comments for MMP4

MMP9: May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. Exists as monomer or homodimer; disulfide-linked. Exists also as heterodimer with a 25 kDa protein. Macrophages and transformed cell lines produce only the monomeric form. Interacts with ECM1. Activated by 4-aminophenylmercuric acetate and phorbol ester. Up-regulated by ARHGEF4, SPATA13 and APC via the JNK signaling pathway in colorectal tumor cells. Produced by normal alveolar macrophages and granulocytes. Inhibited by histatin-3 1/24 (histatin-5). Inhibited by ECM1. Belongs to the peptidase M10A family.

Protein type: Protease; Motility/polarity/chemotaxis; Secreted; EC 3.4.24.35; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: proteinaceous extracellular matrix; extracellular space; extracellular region

Molecular Function: collagen binding; identical protein binding; protein binding; zinc ion binding; metalloendopeptidase activity; endopeptidase activity

Biological Process: positive regulation of keratinocyte migration; collagen catabolic process; axon guidance; extracellular matrix disassembly; extracellular matrix organization and biogenesis; ossification; macrophage differentiation; positive regulation of apoptosis; ephrin receptor signaling pathway; proteolysis; skeletal development; leukocyte migration; embryo implantation

Disease: Metaphyseal Anadysplasia 2

Research Articles on MMP4

1. No evidence to support the association of MMP-1-1607 1G/2G and MMP-9-1562 C/T gene polymorphisms with spontaneous preterm birth in European Caucasian women.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.