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PGK2 Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS844966

Background: PGK2 is a testis-specific form of phosphoglycerate kinase. Initially assumed to be a pseudogene, this protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1, 3-bisphosphoglycerate to… More details

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Product Overview

Product Name
PGK2, Active Protein
Full Product Name
PGK2, human recombinant
Product Gene Name
PGK2 active protein
Product Synonym Names
Phosphoglycerate kinase 2; dJ417L20.2; PGKB; PGKPSS
Sequence
MGSSHHHHHH SSGLVPRGSH MSLSKKLTLD KLDVRGKRVI MRVDFNVPMK KNQITNNQRI KASIPSIKYC LDNGAKAVVL MSHLGRPDGV PMPDKYSLAP VAVELKSLLG KDVLFLKDCV GAEVEKACAN PAPGSVILLE NLRFHVEEEG KGQDPSGKKI KAEPDKIEAF RASLSKLGDV YVNDAFGTAH RAHSSMVGVN LPHKASGFLM KKELDYFAKA LENPVRPFLA ILGGAKVADK IQLIKNMLDK VNEMIIGGGM AYTFLKVLNN MEIGASLFDE EGAKIVKDIM AKAQKNGVRI TFPVDFVTGD KFDENAQVGK ATVASGISPG WMGLDCGPES NKNHAQVVAQ ARLIVWNGPL GVFEWDAFAK GTKALMDEIV KATSKGCITV IGGGDTATCC AKWNTEDKVS HVSTGGGASL ELLEGKILPG VEALSNM
Host
E Coli
Form/Format
0.5 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1 M NaCl and 1 mM DTT
Appearance: Liquid
Purity/Purification
>=85%
Concentration
0.5 mg/ml (lot specific)
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Molecular Weight
46.9 kDa (437 aa, 1-417 aa + His Tag)
OMIM
172270
3D Structure
ModBase 3D Structure for P07205
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Biological Activity
Specific activity is >11 units/mg. One unit will convert 1.0 umole of beta-NADH per minute in a coupled system with GAPDH at pH 8.0 at 25 degree C
Results
Specific activity is >11 units/mg
Unit Definition
One unit will convert 1.0 umole of beta-NADH per minute in a coupled system with GAPDH at pH 8.0 at 25 degree C
Handling
Centrifuge the vial prior to opening.

Product Categories/Family for PGK2 active protein

Proteins and Enzymes; Proteins and Enzymes (A-Z)
Applications Tested/Suitable for PGK2 active protein
SDS-PAGE

Testing Data of PGK2 active protein

PGK2 Active Protein – Cat# MBS844966

Other Notes

Small volumes of PGK2 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PGK2 purchase

MBS844966 is an active protein targeting PGK2. Also known as PGK2, human recombinant. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for PGK2. It may not necessarily be applicable to this product.

NCBI GI #
31543397
NCBI GeneID
5232
NCBI Accession #
NP_620061.2
UniProt Primary Accession #
P07205
UniProt Secondary Accession #
Q9H107; B2R6Y8
UniProt Related Accession #
P07205
GenBank Nucleotide #
NM_138733.4
NCBI Official Full Name
phosphoglycerate kinase 2
NCBI Official Synonym Full Names
phosphoglycerate kinase 2
NCBI Official Symbol
PGK2
NCBI Official Synonym Symbols
PGKB; PGKPS; HEL-S-272; dJ417L20.2
UniProt Protein Name
Phosphoglycerate kinase 2
UniProt Synonym Protein Names
Phosphoglycerate kinase, testis specific
Protein Family
2-phosphoglycerate kinase
UniProt Gene Name
PGK2
UniProt Synonym Gene Names
PGKB
UniProt Entry Name
PGK2_HUMAN
NCBI Protein Information
phosphoglycerate kinase 2

NCBI Summary for PGK2

This gene is intronless, arose via retrotransposition of the phosphoglycerate kinase 1 gene, and is expressed specifically in the testis. Initially assumed to be a pseudogene, the encoded protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis.[provided by RefSeq, May 2010]
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UniProt Comments for PGK2

PGK2: This gene is intronless, arose via retrotransposition of the phosphoglycerate kinase 1 gene, and is expressed specifically in the testis. Initially assumed to be a pseudogene, the encoded protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis.[provided by RefSeq, May 2010]

Protein type: EC 2.7.2.3; Kinase, other; Carbohydrate Metabolism - glycolysis and gluconeogenesis

Chromosomal Location of Human Ortholog: 6p12.3

Cellular Component: cytoplasm; nucleus

Molecular Function: phosphoglycerate kinase activity; ATP binding

Biological Process: sperm motility; glycolysis; phosphorylation
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Research Articles on PGK2

1. Data from studies in transgenic mice suggest that Pgk2 core promoter elements are required to direct demethylation of DNA and reconfiguration of nucleosomes in testis during sexual maturation and spermatogenesis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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