Fc Gamma RIIB/Cd32b (FCGR2B) Active Protein

Name: FCGR2B Active Protein; Fc Gamma RIIB/Cd32b; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS844984
Price: 320 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS844984

Background: Receptors for the Fc region of IgG (Fc gamma R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc gamma Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

Fc gamma RIIB/CD32b (FCGR2B), Active Protein

Full Product Name

Human CellExp Fc gamma RIIB / CD32b, human recombinant

Product Gene Name

FCGR2B active protein

Product Synonym Names

FCGR2B; CD32b; CD32; FCG2; IGFR2; CDw32

Host

HEK293 cells

Form/Format

Lyophilized from 0.22 mum filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Appearance: Lyophilized

Purity/Purification

>=95%

Preparation and Storage

At -20 degree C
Shelf Life: 12 months

Molecular Weight

This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 20.2 kDa. The predicted N-terminus is Ala 46. DTT-reduced Protein migrates as 23-33 kDa due to different glycosylation.

OMIM

152700

3D Structure

ModBase 3D Structure for P31994

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Solubility/Reconstitution Instructions

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 ug/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 month. For extended storage, it is recommended to store at -20 degree C.

Endotoxin Level

<1 EU/mug by LAL method

Biological Activity

Measured by its ability to bind human IgG with an estimated Kd <150 nM.

Results

Measured by its ability to bind human IgG with an estimated Kd <150 nM.

Handling

Centrifuge the vial prior to opening.

Product Categories/Family for FCGR2B active protein

Proteins and Enzymes; Human cell expressed recombinant proteinsProteins and Enzymes; Cytokines; Growth Factors & Hormones; CDsProteins and Enzymes; Cytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A-Z) Proteins and Enzymes; Proteins and E

Applications Tested/Suitable for FCGR2B active protein

SDS-PAGE

Other Notes

Small volumes of FCGR2B active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FCGR2B purchase

MBS844984 is an active protein targeting FCGR2B. Also known as Human CellExp Fc gamma RIIB / CD32b, human recombinant. Host: HEK293 cells.

Additional Details

NCBI/Uniprot data below describe general gene information for FCGR2B. It may not necessarily be applicable to this product.

NCBI GI #

50511932

NCBI GeneID

2213

NCBI Accession #

NP_001002273.1

UniProt Primary Accession #

P31994

UniProt Secondary Accession #

O95649; Q53X85; Q5VXA9; Q8NIA1; A6H8N3

UniProt Related Accession #

P31994

GenBank Nucleotide #

NM_001002273.2

NCBI Official Full Name

low affinity immunoglobulin gamma Fc region receptor II-b isoform 2

NCBI Official Synonym Full Names

Fc fragment of IgG, low affinity IIb, receptor (CD32)

NCBI Official Symbol

FCGR2B

NCBI Official Synonym Symbols

CD32; FCG2; CD32B; FCGR2; IGFR2

UniProt Protein Name

Low affinity immunoglobulin gamma Fc region receptor II-b

UniProt Synonym Protein Names

CDw32; Fc-gamma RII-b; Fc-gamma-RIIb; FcRII-b; CD_antigen: CD32

Protein Family

Low affinity immunoglobulin gamma Fc region receptor

UniProt Gene Name

FCGR2B

UniProt Synonym Gene Names

CD32; FCG2; IGFR2; IgG Fc receptor II-b; Fc-gamma-RIIb; FcRII-b

UniProt Entry Name

FCG2B_HUMAN

NCBI Protein Information

low affinity immunoglobulin gamma Fc region receptor II-b

NCBI Summary for FCGR2B

The protein encoded by this gene is a low affinity receptor for the Fc region of immunoglobulin gamma complexes. The encoded protein is involved in the phagocytosis of immune complexes and in the regulation of antibody production by B-cells. Variations in this gene may increase susceptibilty to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]

UniProt Comments for FCGR2B

FCGR2B: a transmembrane receptor for the Fc region of complexed or aggregated immunoglobulin gamma (IgG). Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down modulation of previous state of cell activation triggered via antigen receptors on B cells, T cells or via another Fc receptor. Three splice-variant isoforms have been observed.

Protein type: Oncoprotein; Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 1q23

Cellular Component: integral to membrane; plasma membrane

Molecular Function: protein binding; IgG binding

Biological Process: regulation of immune response; viral reproduction; immune response; signal transduction

Disease: Systemic Lupus Erythematosus; Malaria, Susceptibility To

Research Articles on FCGR2B

1. FCGR2B inhibitory gene may be predictive of adjuvant trastuzumab benefit in HER2+ breast cancer patients.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.