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High Mobility Group Protein 1 (HMG1) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2105091
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MBS2105091 is an active protein targeting HMG1. Also known as Active High Mobility Group Protein 1 (HMG1). Host: 293F cell. More details

$325 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
High Mobility Group Protein 1 (HMG1), Active Protein
Full Product Name
Active High Mobility Group Protein 1 (HMG1)
Product Gene Name
HMG1 active protein
Product Synonym Names
HMGB1; HMG3; SBP1; Sulfoglucuronyl Carbohydrate Binding Protein; Amphoterin; High Mobility Group Box 1 Protein
Host
293F cell
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 5%Trehalose.
Purity/Purification
>95%
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8°C for one month.
Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Molecular Weight
Predicted Molecular Mass: 51.2kDa
Accurate Molecular Mass: 57kDa as determined by SDS-PAGE reducing conditions.
OMIM
163905
3D Structure
ModBase 3D Structure for P09429
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Organism Species
Homo sapiens (Human)
Source
Eukaryotic expression.
Residues
Met1~Glu215
Tags
N-terminal His-tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method).
Predicted isoelectric point
5.6
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Product Categories/Family for HMG1 active protein

Infection immunity
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for HMG1 active protein
Cell Culture, Activity Assays
(May be suitable for use in other assays to be determined by the end user.)

Sequence of HMG1 active protein

HMG1 Active Protein – Cat# MBS2105091

Gene Sequence of HMG1 active protein

Gene Sequencing (extract)
HMG1 Active Protein, Gene Sequence – Cat# MBS2105091

SDS-PAGE of HMG1 active protein

Sample: Active recombinant HMG1, Human
HMG1 Active Protein, SDS-PAGE – Cat# MBS2105091

Western Blot (WB) of HMG1 active protein

Sample: Recombinant HMG1, Human;
Antibody: Rabbit Anti-Human HMG1 Ab (“Please Inquire”)
HMG1 Active Protein, Western Blot (WB) – Cat# MBS2105091

Activity of HMG1 active protein

High Mobility Group Protein 1 (HMG1), also known as high mobility group box 1 proteinbelongs to high mobility group and contains HMG-box domain. HMG1 is one of the most important chromatin proteins. This nuclear protein organizes the DNA and regulates transcription. It supports transcription of many genes in interactions with many transcription factors. HMGB1 is secreted by immune cells (like macrophages, monocytes and dendritic cells) through leaderless secretory pathway. Activated macrophages and monocytes secrete HMGB1 as a cytokine mediator of Inflammation. Besides, Tumor Protein p53 (TP53) has been identified as an interactor of HMG1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human HMG1 and recombinant human TP53. Briefly, HMG1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to TP53-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-HMG1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of HMG1 and TP53 was shown in Figure 1, and this effect was in a dose dependent manner.
HMG1 Active Protein, Activity – Cat# MBS2105091

Other Notes

Small volumes of HMG1 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for HMG1. It may not necessarily be applicable to this product.

NCBI GI #
4504425
NCBI GeneID
3146
NCBI Accession #
NP_002119.1
UniProt Primary Accession #
P09429
UniProt Related Accession #
P09429
GenBank Nucleotide #
NM_002128.7
NCBI Official Full Name
high mobility group protein B1 isoform 1
NCBI Official Synonym Full Names
high mobility group box 1
NCBI Official Symbol
HMGB1
NCBI Official Synonym Symbols
HMG1; HMG3; HMG-1; SBP-1
UniProt Protein Name
High mobility group protein B1
UniProt Synonym Protein Names
High mobility group protein 1
Protein Family
3-hydroxy-3-methylglutaryl-coenzyme A reductase
UniProt Gene Name
HMGB1
UniProt Synonym Gene Names
HMG1; HMG-1
UniProt Entry Name
HMGB1_HUMAN
NCBI Protein Information
high mobility group protein B1

NCBI Summary for HMG1

This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]
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UniProt Comments for HMG1

HMGB1: DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2. Belongs to the HMGB family.

Protein type: DNA repair, damage; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 13q12

Cellular Component: nucleoplasm; extracellular space; cell surface; transcriptional repressor complex; extracellular region; condensed chromosome; nucleus

Molecular Function: protein binding; RAGE receptor binding; double-stranded DNA binding; damaged DNA binding; cytokine activity; chromatin binding; transcription factor activity; DNA bending activity; transcription factor binding; single-stranded DNA binding; chemoattractant activity

Biological Process: negative regulation of transcriptional preinitiation complex assembly; DNA topological change; V(D)J recombination; apoptosis; positive regulation of apoptosis; positive regulation of caspase activity; base-excision repair, DNA ligation; negative regulation of transcription from RNA polymerase II promoter; DNA recombination; chromatin remodeling; regulation of transcription from RNA polymerase II promoter; myeloid dendritic cell activation; inflammatory response to antigenic stimulus; positive chemotaxis; dendritic cell chemotaxis; innate immune response; DNA ligation during DNA repair; positive regulation of transcription from RNA polymerase II promoter; DNA fragmentation during apoptosis; neurite development; cell structure disassembly during apoptosis; positive regulation of DNA binding
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Research Articles on HMG1

1. High expression of HMGB1 was associated with MVD and poor prognosis in clinical analyzation. Postoperative serum HMGB1 and cholangitis could predict high recurrence and unfavorable prognosis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with HMG1 active protein

 Reference Product  PubMed Publications
 TLR4 active protein  >231 publications with HMG1 and TLR4
 TLR2 active protein  >99 publications with HMG1 and TLR2
 CASP3 active protein  >65 publications with HMG1 and CASP3
 RAG1 active protein  >21 publications with HMG1 and RAG1
 TBP active protein  >12 publications with HMG1 and TBP
 RELA active protein  >9 publications with HMG1 and RELA
 TP53 active protein  >5 publications with HMG1 and TP53
 PRKDC active protein  >5 publications with HMG1 and PRKDC
 POU5F1 active protein  >3 publications with HMG1 and POU5F1
 NCAN active protein  >3 publications with HMG1 and NCAN

Pathways associated with HMG1 active protein

 Products by Pathway  Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Activation Of DNA Fragmentation Factor Pathway antibodies  Activation Of DNA Fragmentation Factor Pathway Diagram
 Advanced Glycosylation Endproduct Receptor Signaling Pathway antibodies  Advanced Glycosylation Endproduct Receptor Signaling Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Induced DNA Fragmentation Pathway antibodies  Apoptosis Induced DNA Fragmentation Pathway Diagram
 Apoptotic Execution Phase Pathway antibodies  Apoptotic Execution Phase Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 Cytosolic Sensors Of Pathogen-associated DNA Pathway antibodies  Cytosolic Sensors Of Pathogen-associated DNA Pathway Diagram
 DAI Mediated Induction Of Type I IFNs Pathway antibodies  DAI Mediated Induction Of Type I IFNs Pathway Diagram

Diseases associated with HMG1 active protein

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >964 publications with HMG1 and Inflammation
 Necrosis Antibodies  >557 publications with HMG1 and Necrosis
 Sepsis Antibodies  >397 publications with HMG1 and Sepsis
 Neoplasms Antibodies  >379 publications with HMG1 and Neoplasms
 Disease Models, Animal Antibodies  >274 publications with HMG1 and Disease Models, Animal
 Lung Diseases Antibodies  >160 publications with HMG1 and Lung Diseases
 Liver Diseases Antibodies  >115 publications with HMG1 and Liver Diseases
 Drug Toxicity Antibodies  >85 publications with HMG1 and Drug Toxicity
 Heart Diseases Antibodies  >82 publications with HMG1 and Heart Diseases
 Myocardial Ischemia Antibodies  >70 publications with HMG1 and Myocardial Ischemia

Organs/Tissues associated with HMG1 active protein

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >842 publications with HMG1 and Blood
 Liver Antibodies  >304 publications with HMG1 and Liver
 Lung Antibodies  >272 publications with HMG1 and Lung
 Brain Antibodies  >216 publications with HMG1 and Brain
 Vascular Antibodies  >214 publications with HMG1 and Vascular
 Muscle Antibodies  >127 publications with HMG1 and Muscle
 Heart Antibodies  >114 publications with HMG1 and Heart
 Kidney Antibodies  >108 publications with HMG1 and Kidney
 Nerve Antibodies  >92 publications with HMG1 and Nerve
 Bone Antibodies  >91 publications with HMG1 and Bone
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