Human Recombinant MDH1 Active Protein – Cat# MBS845028

MDH1 Human Recombinant Active Protein

Name: MDH1 Active Protein; Human Recombinant; MyBioSource
Brand: MyBioSource
SKU: MBS845028
Price: 555 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS845028

Background: Malate Dehydrogenase, Cytoplasmic (MDH1) is an enzyme which belongs to the MDH Type 2 sub-family of LDH/MDH superfamily. MDH1 is involved in the Citric Acid Cycle that catalyzes the conversion of Malate into Oxaloacetate (using NAD+) and vice… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

MDH1, Active Protein

Full Product Name

Human Recombinant MDH1

Product Gene Name

MDH1 active protein

Product Synonym Names

Malate dehydrogenase cytoplasmic; MDH-s; MDHA; MOR2; Human Recombinant MDH1

Sequence

MSEPIRVLVT GAAGQIAYSL LYSIGNGSVF GKDQPIILVL LDITPMMGVL DGVLMELQDC ALPLLKDVIA TDKEDVAFKD LDVAILVGSM PRREGMERKD LLKANVKIFK SQGAALDKYA KKSVKVIVVG NPANTNCLTA SKSAPSIPKE NFSCLTRLDH NRAKAQIALK LGVTANDVKN VIIWGNHSST QYPDVNHAKV KLQGKEVGVY EALKDDSWLK GEFVTTVQQR GAAVIKARKL SSAMSAAKAI CDHVRDIWFG TPEGEFVSMG VISDGNSYGV PDDLLYSFPV VIKNKTWKFV EGLPINDFSR EKMDLTAKEL TEEKESAFEF LSSALEHHHH HH

Host

E Coli

Form/Format

1 mg/ml solution in 20 mM Tris-HCl (pH 8.0) containing 10% glycerol.
Appearance: Liquid

Purity/Purification

>=95%

Concentration

1 mg/ml (lot specific)

Preparation and Storage

At -80 degree C
Shelf Life: 12 months

Molecular Weight

37.4 kDa (342 aa, 1-334 aa + CT His Tag)

OMIM

154200

3D Structure

ModBase 3D Structure for P40925

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Biological Activity

Specific activity is > 8 units/mg, and is defined as the amount of enzyme that cleaves 1 umole of oxaloacetate and beta-NADH to L-malate and beta-NAD per minute at pH 7.5 at 25 degree C.

Unit Definition

Specific activity is defined as the amount of enzyme that cleaves 1 umole of oxaloacetate and beta-NADH to L-malate and beta-NAD per minute at pH 7.5 at 25 degree C.

Handling

Centrifuge the vial prior to opening.

Product Categories/Family for MDH1 active protein

Proteins and Enzymes; Proteins and Enzymes (A-Z)

Applications Tested/Suitable for MDH1 active protein

SDS-PAGE

Other Notes

Small volumes of MDH1 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MDH1 purchase

MBS845028 is an active protein targeting MDH1. Also known as Human Recombinant MDH1. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for MDH1. It may not necessarily be applicable to this product.

NCBI GI #

312283701

NCBI GeneID

4190

NCBI Accession #

NP_001186040.1

UniProt Primary Accession #

P40925

UniProt Secondary Accession #

Q6I9V0; B2R5V5; B4DUN2; B7Z3I7; F5H098

UniProt Related Accession #

P40925

GenBank Nucleotide #

NM_001199111.1

NCBI Official Full Name

malate dehydrogenase, cytoplasmic isoform 1

NCBI Official Synonym Full Names

malate dehydrogenase 1, NAD (soluble)

NCBI Official Symbol

MDH1

NCBI Official Synonym Symbols

MDHA; MOR2; MDH-s; HEL-S-32; MGC:1375

UniProt Protein Name

Malate dehydrogenase, cytoplasmic

UniProt Synonym Protein Names

Cytosolic malate dehydrogenase; Diiodophenylpyruvate reductase (EC:1.1.1.96)

Protein Family

Malate dehydrogenase

UniProt Gene Name

MDH1

UniProt Synonym Gene Names

MDHA

UniProt Entry Name

MDHC_HUMAN

NCBI Protein Information

malate dehydrogenase, cytoplasmic

NCBI Summary for MDH1

Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Nov 2010]

UniProt Comments for MDH1

MDH1: Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Nov 2010]

Protein type: Carbohydrate Metabolism - glyoxylate and dicarboxylate; Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - citrate (TCA) cycle; EC 1.1.1.96; EC 1.1.1.37; Oxidoreductase

Chromosomal Location of Human Ortholog: 2p13.3

Cellular Component: extracellular space; centrosome; mitochondrion; cytoplasm; cytosol

Molecular Function: malic enzyme activity; L-malate dehydrogenase activity; diiodophenylpyruvate reductase activity; NAD binding

Biological Process: malate metabolic process; oxaloacetate metabolic process; NADH metabolic process; tricarboxylic acid cycle; carbohydrate metabolic process; glucose metabolic process; pathogenesis; cellular carbohydrate metabolic process; gluconeogenesis

Research Articles on MDH1

1. adipogenic differentiation may be regulated by the acetylation of MDH1

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with MDH1 active protein

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>6 publications with MDH1 and Nervous System Diseases
Brain Diseases Antibodies	>4 publications with MDH1 and Brain Diseases
Disease Models, Animal Antibodies	>3 publications with MDH1 and Disease Models, Animal
Vascular Diseases Antibodies	>2 publications with MDH1 and Vascular Diseases
Heart Diseases Antibodies	>2 publications with MDH1 and Heart Diseases
Prenatal Injuries Antibodies	>1 publications with MDH1 and Prenatal Injuries
Seizures Antibodies	>1 publications with MDH1 and Seizures
Neurobehavioral Manifestations Antibodies	>1 publications with MDH1 and Neurobehavioral Manifestations
Kidney Diseases Antibodies	>1 publications with MDH1 and Kidney Diseases
Fibrosis Antibodies	>1 publications with MDH1 and Fibrosis

Organs/Tissues associated with MDH1 active protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>15 publications with MDH1 and Blood
Brain Antibodies	>10 publications with MDH1 and Brain
Muscle Antibodies	>7 publications with MDH1 and Muscle
Liver Antibodies	>5 publications with MDH1 and Liver
Heart Antibodies	>4 publications with MDH1 and Heart
Nerve Antibodies	>3 publications with MDH1 and Nerve
Intestine Antibodies	>3 publications with MDH1 and Intestine
Kidney Antibodies	>2 publications with MDH1 and Kidney
Lung Antibodies	>2 publications with MDH1 and Lung
Pancreas Antibodies	>1 publications with MDH1 and Pancreas