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GASP-1 (GASP) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS845031

Background: Growth and differentiation factor-associated serum protein-1 (GASP-1) is a secreted inhibitory TGF-beta binding protein that contains multiple protease inhibitor structural domains. It is expressed primarily in the ovary, testis, and brain, and… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
GASP-1, Active Protein
Full Product Name
GASP-1, human recombinant
Product Gene Name
GASP active protein
Product Synonym Names
GDF-associated serum protein-1; GASP; KIAA0443; WFIKKNRP
Sequence
LPPIRYSHAG ICPNDMNPNL WVDAQSTCRR ECETDQECET YEKCCPNVCG TKSCVAARYM DVKGKKGPVG MPKEATCDHF MCLQQGSECD IWDGQPVCKC KDRCEKEPSF TCASDGLTYY NRCYMDAEAC SKGITLAVVT CRYHFTWPNT SPPPPETTMH PTTASPETPE LDMAAPALLN NPVHQSVTMG ETVSFLCDVV GRPRPEITWE KQLEDRENVV MRPNHVRGNV VVTNIAQLVI YNAQLQDAGI YTCTARNVAG VLRADFPLSV VRGHQAAATS ESSPNGTAFP AAECLKPPDS EDCGEEQTRW HFDAQANNCL TFTFGHCHRN LNHFETYEAC MLACMSGPLA ACSLPALQGP CKAYAPRWAY NSQTGQCQSF VYGGCEGNGN NFESREACEE SCPFPRGNQR CRACKPRQKL VTSFCRSDFV ILGRVSELTE EPDSGRALVT VDEVLKDEKM GLKFLGQEPL EVTLLHVDWA CPCPNVTVSE MPLIIMGEVD GGMAMLRPDS FVGASSARRV RKLREVMHKK TCDVLKEFLG LH
Host
CHO Cells
Form/Format
Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM sodium phosphate, pH 7.8.
Appearance: Lyophilized powder
Purity/Purification
>=95%
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Molecular Weight
55-66 kDa
OMIM
300417
3D Structure
ModBase 3D Structure for Q5JY77
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Solubility/Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20 degree C to -80 degree C.
Endotoxin Level
< 0.1 ng/mug of protein (<1EU/mug).
Activity (Specifications/test method)
Determined by its ability to inhibit human Myostatin (GDF-8) activity in MCP-11 cells. The ED50 for this effect is 0.0025-0.0040 ug/ml in the presence of 5ng/ml of human Myostatin (GDF-8)
Biological Activity
Determined by its ability to inhibit human Myostatin (GDF-8) activity in MCP-11 cells. The ED50 for this effect is 0.0025-0.0040 ug/ml in the presence of 5ng/ml of human Myostatin (GDF-8)
Handling
Centrifuge the vial prior to opening.

Product Categories/Family for GASP active protein

Cytokines; Growth Factors & Hormones; TGFsCytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A-Z) Proteins and Enzymes; Cytokines; Growth Factors & Hormones; TGFsProteins and Enzymes; Cytokines; Growth Factors & Hormones; Cytokines & Growth
Applications Tested/Suitable for GASP active protein
SDS-PAGE; HPLC

Other Notes

Small volumes of GASP active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GASP purchase

MBS845031 is an active protein targeting GASP. Also known as GASP-1, human recombinant. Host: CHO Cells.

Additional Details

NCBI/Uniprot data below describe general gene information for GASP. It may not necessarily be applicable to this product.

NCBI GI #
150378489
NCBI GeneID
9737
NCBI Accession #
NP_001092880.1
UniProt Primary Accession #
Q5JY77
UniProt Secondary Accession #
O43168; Q96LA1
UniProt Related Accession #
Q5JY77
GenBank Nucleotide #
NM_001099410.1
NCBI Official Full Name
G-protein coupled receptor-associated sorting protein 1
NCBI Official Synonym Full Names
G protein-coupled receptor associated sorting protein 1
NCBI Official Symbol
GPRASP1
NCBI Official Synonym Symbols
GASP; GASP1; GASP-1
UniProt Protein Name
G-protein coupled receptor-associated sorting protein 1
Protein Family
G-protein coupled receptor-associated sorting protein
UniProt Gene Name
GPRASP1
UniProt Synonym Gene Names
GASP; KIAA0443; GASP-1
UniProt Entry Name
GASP1_HUMAN
NCBI Protein Information
G-protein coupled receptor-associated sorting protein 1

NCBI Summary for GASP

This gene encodes a member of the GPRASP (G protein-coupled receptor associated sorting protein) family. The protein may modulate lysosomal sorting and functional down-regulation of a variety of G-protein coupled receptors. It targets receptors for degradation in lysosomes. The receptors interacting with this sorting protein include D2 dopamine receptor (DRD2), delta opioid receptor (OPRD1), beta-2 adrenergic receptor (ADRB2), D4 dopamine receptor (DRD4) and cannabinoid 1 receptor (CB1R). Multiple alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, May 2010]
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UniProt Comments for GASP

GPRASP1: May modulate lysosomal sorting and functional down- regulation of a variety of G-protein coupled receptors. Targets receptors for degradation in lysosomes. Belongs to the GPRASP family.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: Xq22.1

Cellular Component: cytoplasm

Molecular Function: protein binding

Biological Process: endosome to lysosome transport
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Research Articles on GASP

1. Data suggest that specific serine/threonine residues in DRD2 (dopamine receptor D2) are involved in regulation of DRD2 endocytosis by GRK2 (G-protein coupled receptor kinase 2) and GASP1.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with GASP active protein

 Disease Name  Pubmed Publications
 Brain Diseases Antibodies  >1 publications with GASP and Brain Diseases
 Hypertrophy Antibodies  >1 publications with GASP and Hypertrophy
 Hyperplasia Antibodies  >1 publications with GASP and Hyperplasia

Organs/Tissues associated with GASP active protein

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >3 publications with GASP and Brain
 Nerve Antibodies  >1 publications with GASP and Nerve
 Kidney Antibodies  >1 publications with GASP and Kidney
 Eye Antibodies  >1 publications with GASP and Eye
 Muscle Antibodies  >1 publications with GASP and Muscle
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