Figure. Cell proliferation of 3T3 cells after stimulated with EGF.Epidermal growth factor (EGF) binding to EGFR, results in cellular proliferation, differentiation, and survival. To test the effect of EGF on cell proliferation, 3T3 fibroblasts cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach, replaced with serum-free overnight, then the medium was replaced with 1% serum standard DMEM prior to the addition of various concentrations of recombinant rat EGF. After incubated for 96h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Proliferation of 3T3 cells after incubation with EGF for 96h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant EGF for 96h. The result was shown in Figure 2. It was obvious that EGF significantly increased cell viability of 3T3 cells.(A) 3T3 cells cultured in DMEM, stimulated with 1ng/mL EGF for 96h;(B) Unstimulated 3T3 cells cultured in DMEM for 96h.

Epidermal Growth Factor (EGF) Active Protein

Name: EGF Active Protein; Epidermal Growth Factor; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS2105104
Price: 275 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2105104

MBS2105104 is an active protein targeting EGF. Also known as Active Epidermal Growth Factor (EGF). More details

$275 / 0.01 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Epidermal Growth Factor (EGF), Active Protein

Full Product Name

Active Epidermal Growth Factor (EGF)

Product Gene Name

EGF active protein

Product Synonym Names

URG; Beta-Urogastrone; Pro-epidermal growth factor

Form/Format

Freeze-Dried Powder; 20mM Tris, 150mM NaCl, pH8.0, Containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.

Purity/Purification

> 95%

Preparation and Storage

Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Avoid repeated freeze/thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

3D Structure

ModBase 3D Structure for P07522

Supply Chain Verification

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Species

Rat

Tag

No-Tag

Isoelectric Point

6.7

Subcellular Location

Membrane; Single-Pass Type I Membrane Protein

Reconstitution

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

ISO Certification

Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.

Applications Tested/Suitable for EGF active protein

Cell Culture, Activity Assays

Other Notes

Small volumes of EGF active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for EGF. It may not necessarily be applicable to this product.

NCBI GI #

6978797

NCBI GeneID

25313

NCBI Accession #

NP_036974.1

UniProt Primary Accession #

P07522

UniProt Related Accession #

P07522

GenBank Nucleotide #

NM_012842.1

NCBI Official Full Name

pro-epidermal growth factor

NCBI Official Synonym Full Names

epidermal growth factor

NCBI Official Symbol

Egf

UniProt Protein Name

Pro-epidermal growth factor

Protein Family

Pro-epidermal growth factor

UniProt Gene Name

Egf

UniProt Synonym Gene Names

EGF

UniProt Entry Name

EGF_RAT

NCBI Protein Information

pro-epidermal growth factor

NCBI Summary for EGF

binds the epidermal growth factor receptor; may play a role in MAP kinase mediated signaling pathways [RGD, Feb 2006]

UniProt Comments for EGF

EGF: EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Defects in EGF are the cause of hypomagnesemia type 4 (HOMG4); also known as renal hypomagnesemia normocalciuric. HOMG4 is a disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion. Clinical features include seizures, mild-to mederate psychomotor retardation, and brisk tendon reflexes. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligand, receptor tyrosine kinase; Hormone; Membrane protein, integral

Cellular Component: extracellular space; lysosomal membrane; plasma membrane; integral to membrane; intracellular

Molecular Function: growth factor activity; calcium ion binding; epidermal growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; peptidyl-tyrosine phosphorylation; activation of MAPKK activity; positive regulation of mitosis; positive regulation of transcription, DNA-dependent; regulation of protein transport; positive regulation of MAP kinase activity; regulation of protein secretion; branching morphogenesis of a tube; positive regulation of epidermal growth factor receptor activity; positive regulation of cell proliferation; negative regulation of secretion; angiogenesis; regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphorylation; STAT protein nuclear translocation; positive regulation of DNA binding; positive regulation of granule cell precursor proliferation

Research Articles on EGF

1. The expression of EGF was elevated on the first day after tympanic membrane (TM)injury, not reaching statistical significance, and then returned to the level observed in the control TM.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with EGF active protein

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Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
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Cardiovascular Signaling Pathway antibodies	Cardiovascular Signaling Pathway Diagram
Choline Metabolism In Cancer Pathway antibodies	Choline Metabolism In Cancer Pathway Diagram
Choline Metabolism In Cancer Pathway antibodies	Choline Metabolism In Cancer Pathway Diagram
Constitutive PI3K/AKT Signaling In Cancer Pathway antibodies	Constitutive PI3K/AKT Signaling In Cancer Pathway Diagram
Constitutive Signaling By EGFRvIII Pathway antibodies	Constitutive Signaling By EGFRvIII Pathway Diagram
Constitutive Signaling By Ligand-Responsive EGFR Cancer Variants Pathway antibodies	Constitutive Signaling By Ligand-Responsive EGFR Cancer Variants Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram

Diseases associated with EGF active protein

Disease Name	Pubmed Publications
Breast Neoplasms Antibodies	>1690 publications with EGF and Breast Neoplasms
Adenocarcinoma Antibodies	>1333 publications with EGF and Adenocarcinoma
Necrosis Antibodies	>1008 publications with EGF and Necrosis
Neoplasms, Experimental Antibodies	>896 publications with EGF and Neoplasms, Experimental
Cell Transformation, Neoplastic Antibodies	>877 publications with EGF and Cell Transformation, Neoplastic
Lung Neoplasms Antibodies	>860 publications with EGF and Lung Neoplasms
Inflammation Antibodies	>741 publications with EGF and Inflammation
Neoplasm Metastasis Antibodies	>650 publications with EGF and Neoplasm Metastasis
Liver Diseases Antibodies	>578 publications with EGF and Liver Diseases
Neoplasm Invasiveness Antibodies	>564 publications with EGF and Neoplasm Invasiveness

Organs/Tissues associated with EGF active protein

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>1969 publications with EGF and Liver
Brain Antibodies	>1882 publications with EGF and Brain
Kidney Antibodies	>1618 publications with EGF and Kidney
Prostate Antibodies	>728 publications with EGF and Prostate
Connective Tissue Antibodies	>464 publications with EGF and Connective Tissue
Artery Antibodies	>436 publications with EGF and Artery
Pineal Gland Antibodies	>10 publications with EGF and Pineal Gland
Uncharacterized Tissue Antibodies	>5 publications with EGF and Uncharacterized Tissue