TIGAR Recombinant Protein

Name: TIGAR Recombinant Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS845042
Price: 275 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS845042

Background: TIGAR is a p53-inducible enzyme that catalyzes the hydrolysis of fructose-2-6 bisphosphate (F-2-6-BP) to fructose-6-phosphate and inorganic phosphate. F-2-6-BP is a powerful activator of 6-phosphofructose-1 kinase, the rate limiting enzyme of… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

TIGAR, Recombinant Protein

Full Product Name

TIGAR, human recombinant

Product Gene Name

TIGAR recombinant protein

Product Synonym Names

TP53-induced glycolysis and apoptosis regulator (TIGAR)

Sequence

ARFALTVVRH GETRFNKEKI IQGQGVDEPL SETGFKQAAA AGIFLNNVKF THAFSSDLMR TKQTMHGILE RSKFCKDMTV KYDSRLRERK YGVVEGKALS ELRAMAKAAR EECPVFTPPG GETLDQVKMR GIDFFEFLCQ LILKEADQKE QFSQGSPSNC LETSLAEIFP LGKNHSSKVN SDSGIPGLAA SVLVVSHGAY MRSLFDYFLT DLKCSLPATL SRSELMSVTP NTGMSLFIIN FEEGREVKPT VQCICMNLQD HLNGLTETR

Host

E Coli

Form/Format

Sterile filtered through a 0.2 micron filter. Lyophilized from 20 mM Tris, pH 8.0, 200 mM NaCl and 1 mM DTT
Appearance: Lyophilized powder

Purity/Purification

>=95%

Preparation and Storage

At -20 degree C
Shelf Life: 12 months

Molecular Weight

29.9 kDa

OMIM

610775

3D Structure

ModBase 3D Structure for Q9NQ88

Solubility/Reconstitution Instructions

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8 degree C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20 degree C to -80 degree C.

Endotoxin Level

< 0.1 ng/mug of protein (<1EU/mug).

Handling

Centrifuge the vial prior to opening.

Product Categories/Family for TIGAR recombinant protein

Cytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A-Z) Proteins and Enzymes; Cytokines; Growth Factors & Hormones; Cytokines & Growth Factors (A-Z) Proteins and Enzymes; Proteins and Enzymes (A-Z) Proteins and Enzymes; Animal-Free Origin

Applications Tested/Suitable for TIGAR recombinant protein

SDS-PAGE; HPLC

Other Notes

Small volumes of TIGAR recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TIGAR purchase

MBS845042 is a recombinant protein targeting TIGAR. Also known as TIGAR, human recombinant. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for TIGAR. It may not necessarily be applicable to this product.

NCBI GI #

9966849

NCBI GeneID

57103

NCBI Accession #

NP_065108.1

UniProt Primary Accession #

Q9NQ88

UniProt Secondary Accession #

B2R840

UniProt Related Accession #

Q9NQ88

GenBank Nucleotide #

NM_020375.2

NCBI Official Full Name

fructose-2,6-bisphosphatase TIGAR

NCBI Official Synonym Full Names

TP53 induced glycolysis regulatory phosphatase

NCBI Official Symbol

TIGAR

NCBI Official Synonym Symbols

FR2BP; C12orf5

UniProt Protein Name

Fructose-2,6-bisphosphatase TIGAR

UniProt Synonym Protein Names

TP53-induced glycolysis and apoptosis regulator

Protein Family

Fructose-2,6-bisphosphatase

UniProt Gene Name

TIGAR

UniProt Synonym Gene Names

C12orf5

UniProt Entry Name

TIGAR_HUMAN

NCBI Protein Information

fructose-2,6-bisphosphatase TIGAR

NCBI Summary for TIGAR

This gene is regulated as part of the p53 tumor suppressor pathway and encodes a protein with sequence similarity to the bisphosphate domain of the glycolytic enzyme that degrades fructose-2,6-bisphosphate. The protein functions by blocking glycolysis and directing the pathway into the pentose phosphate shunt. Expression of this protein also protects cells from DNA damaging reactive oxygen species and provides some protection from DNA damage-induced apoptosis. The 12p13.32 region that includes this gene is paralogous to the 11q13.3 region. [provided by RefSeq, Jul 2008]

UniProt Comments for TIGAR

TIGAR: Fructose-bisphosphatase hydrolyzing fructose-2,6- bisphosphate as well as fructose-1,6-bisphosphate. Inhibits glycolysis by reducing cellular levels of fructose-2,6- bisphosphate. May protect cells against reactive oxygen species and against apoptosis induced by tp53. Belongs to the phosphoglycerate mutase family.

Protein type: EC 3.1.3.46; Phosphatase (non-protein)

Chromosomal Location of Human Ortholog: 12p13.3

Cellular Component: intracellular

Molecular Function: fructose-2,6-bisphosphate 2-phosphatase activity

Biological Process: response to xenobiotic stimulus; dephosphorylation; response to gamma radiation

Research Articles on TIGAR

1. Results revealed that TIGAR inhibits both apoptosis and autophagy.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with TIGAR recombinant protein

Reference Product	PubMed Publications
TP53 recombinant protein	>46 publications with TIGAR and TP53
HK2 recombinant protein	>2 publications with TIGAR and HK2

Pathways associated with TIGAR recombinant protein

Products by Pathway	Pathway Diagram
Central Carbon Metabolism In Cancer Pathway antibodies	Central Carbon Metabolism In Cancer Pathway Diagram
Central Carbon Metabolism In Cancer Pathway antibodies	Central Carbon Metabolism In Cancer Pathway Diagram
Direct P53 Effectors Pathway antibodies	Direct P53 Effectors Pathway Diagram
Fructose And Mannose Metabolism Pathway antibodies	Fructose And Mannose Metabolism Pathway Diagram
Fructose And Mannose Metabolism Pathway antibodies	Fructose And Mannose Metabolism Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
Generic Transcription Pathway antibodies	Generic Transcription Pathway Diagram
TP53 Regulates Metabolic Genes Pathway antibodies	TP53 Regulates Metabolic Genes Pathway Diagram
Transcriptional Regulation By TP53 Pathway antibodies	Transcriptional Regulation By TP53 Pathway Diagram
Fructose 2,6-bisphosphate Biosynthesis Pathway antibodies	Fructose 2,6-bisphosphate Biosynthesis Pathway Diagram

Diseases associated with TIGAR recombinant protein

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>6 publications with TIGAR and Nervous System Diseases
Liver Diseases Antibodies	>5 publications with TIGAR and Liver Diseases
Adenocarcinoma Antibodies	>4 publications with TIGAR and Adenocarcinoma
Breast Neoplasms Antibodies	>4 publications with TIGAR and Breast Neoplasms
Liver Neoplasms Antibodies	>4 publications with TIGAR and Liver Neoplasms
Necrosis Antibodies	>4 publications with TIGAR and Necrosis
Lung Neoplasms Antibodies	>3 publications with TIGAR and Lung Neoplasms
Inflammation Antibodies	>3 publications with TIGAR and Inflammation
Pain Antibodies	>2 publications with TIGAR and Pain
Fatty Liver Antibodies	>2 publications with TIGAR and Fatty Liver

Organs/Tissues associated with TIGAR recombinant protein

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>8 publications with TIGAR and Brain
Lung Antibodies	>6 publications with TIGAR and Lung
Blood Antibodies	>5 publications with TIGAR and Blood
Muscle Antibodies	>5 publications with TIGAR and Muscle
Pancreas Antibodies	>4 publications with TIGAR and Pancreas
Intestine Antibodies	>2 publications with TIGAR and Intestine
Lymph Node Antibodies	>2 publications with TIGAR and Lymph Node
Lymph Antibodies	>2 publications with TIGAR and Lymph
Testis Antibodies	>1 publications with TIGAR and Testis
Kidney Antibodies	>1 publications with TIGAR and Kidney